LF:BLBC0211c Biochemistry - practice - Course Information
BLBC0211c Biochemistry - practice
Faculty of Medicinespring 2021
- Extent and Intensity
- 0/3/0. 2 credit(s). Type of Completion: z (credit).
Taught partially online. - Teacher(s)
- Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. Martina Drabinová (seminar tutor)
Mgr. David Galuška (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
doc. RNDr. Jiří Dostál, CSc. (alternate examiner)
prof. RNDr. Eva Táborská, CSc. (alternate examiner) - Guaranteed by
- doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine - Timetable
- Thu 4. 3. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 11. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 18. 3. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 25. 3. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 1. 4. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 8. 4. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 15. 4. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 22. 4. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 29. 4. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 6. 5. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 13. 5. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 20. 5. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 27. 5. 8:00–10:30 A16/212, 8:00–10:30 A16/216, Thu 3. 6. 8:00–10:30 A16/216, 8:00–10:30 A16/212, Thu 10. 6. 8:00–10:30 A16/216, 8:00–10:30 A16/212
- Prerequisites (in Czech)
- BLLC0111s Med.Chem.-seminar && BLLC0111c Medical Chemistry -p && ZC011 Handling chemical substances
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Medical Laboratory Technologist (programme LF, B-LABD)
- Laboratory Assistant (programme LF, B-SZ)
- Course objectives
- The aim of the course is to acquaint students with the work with biological material and with the basic methodological principles and procedures used in determining the concentration of analytes and enzyme activity in body fluids and with the basic methods of interpretation of laboratory results.
- Learning outcomes
- After completing the course, the student will be:
- acquainted with the basic methodological principles and procedures used in the clinical laboratory in determining the concentration of analytes and enzyme activity in body fluids.
- able to follow the principles of working with biological material.
- able to orientate in basic laboratory tasks.
- able to interpret the result of laboratory examination. - Syllabus
- Handling biological material. Anticoagulants and their use for blood plasma separation. Communicating results of tests in clinical biochemistry: request forms and laboratory reports.
- Analysis of the absorption spectra of haemoglobin, oxyhaemoglobin and carbonylhaemoglobin. Determination of haemoglobin in blood. Urine test for haemoglobin.
- Determination of total bilirubin in serum. Estimation of bilirubin, urobilinogens and urobilins in urine.
- Determination of total serum proteins by biuret reagent, determination of albumin. Precipitation tests for urine proteins. Detection of proteinuria by diagnostic strips.
- Determination of alanine aminotransferase activity in serum. Determination of alkaline phosphatase activity in serum.
- The enzymatic assay of glucose in blood and urine. Oral glucose tolerance test. Estimation of glycaemia by diagnostic strips. Detection of glucose in urine.
- Determination of total cholesterol in serum. Principles of HDL-cholesterol determination and calculation of the LDL-cholesterol concentration. Chromatography of serum lipids.
- The solubility of uric acid. Murexide test for uric acid. Detection of uric acid in renal calculi. Determination of uric acid in serum and urine.
- Determination of urea in serum and urine. Approximate assessment of the nitrogen balance.
- Appearance and volume of urine, specific gravity and osmolality of urine. Urine pH measurement. Determination of creatinine in blood, serum and urine. Creatinine clearance calculation, calculation of tubular reabsorption and fractional excretion of water.
- Tests in routine urinalysis employing chemical principle: determination of pathological components, multipurpose diagnostic strips in urinalysis.
- Simplified chemical analysis of renal calculi. Practice in urinalysis, seeking for pathological constituents in urine.
- Determination of gastric acid output. Tubeless gastric analyses. Detection of lactic acid.
- Literature
- required literature
- Návody ke cvičením ve Studijních materiálech předmětu v IS.
- Teaching methods
- A Lab exercise.
- Assessment methods
- Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports and handing them to the teacher till the given data, and writing the final test (at least 60% success rate).
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu BLBC0211p.
Information on the extent and intensity of the course: 45. - Listed among pre-requisites of other courses
- Teacher's information
- A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required for the exercise.
A list of all laboratory practice topics is post up on notice board of Department of Biochemistry and also on IS MUNI - Study materials - Course-related instructions.
Consultations are possible after personal arrangement with the teacher.
- Enrolment Statistics (spring 2021, recent)
- Permalink: https://is.muni.cz/course/med/spring2021/BLBC0211c