FaF:FABL1_16 Biotechnology of Drugs - Course Information

FABL1_16 Biotechnology of Drugs

Extent and Intensity

3/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

Mgr. Marie Brázdová, Ph.D. (lecturer)
prof. Ing. René Kizek, DrSc., MBA (lecturer)

Guaranteed by

prof. Ing. René Kizek, DrSc., MBA
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy

Prerequisites

3140/FAOB1 Biology
3120/FAME1 Molecular biology
3120/FAMG1 Methods of molecular biology

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Pharmacy (programme FaF, M-FARMA)

Course objectives

The course is demonstration of Pharmaceutical Biotechnology - scientific discipline focusing on all technologies required for the production, manufacturing and registration of biotechnological drugs. Students will be introduced to processes which are used by organisms or their parts to production or modification of drugs. The methods used for breeding of animals, plants and microorganisms usable in medicine and pharmacy will be discussed. The main focus is done for recombinant techniques, gene and protein engineering and gene therapy. Products of industrial biotechnology as antibiotics, vitamins, organic acids, amino acids and alkaloids, will be presented.

Learning outcomes

Basic knowledge from cytology, gene engineering and industrial microbiology usable in recombinant drugs design, development and production. View to biotechnological production of antibiotics, vitamins, organic acids, amino acids and alkaloids.

Syllabus

• 1) Introduction to pharmaceutical biotechnology, definition, milestones
  2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones
  3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors
  4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification
  5) Expression of recombinant proteins in prokaryotic cells and yeasts
  6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses
  7) The classical biotechnological processes in pharmacology
  8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification
  9) Examples of classical and recombinant biotechnology products in pharmacy, cytokines, hormones, enzymes, antibodies and their derivates
  10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes
  11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming
  12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry, rules for genetically modified organisms manipulation
  13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy
  14) New trends in biopharmaceutical products development
  

Literature

required literature
• Brown, T.A. (2010). Gene cloning and DNA analysis, an introduction. 6th edition, Wiley-Blackwell. info
• Alberts et al. (1998). Molecular Biology of the Cell, 5th edition, GS Garland Science. info
• Walsh, G. (2007). Pharmaceutical Biotechnology, John Wiley and sons. info
• HO, R. J. Y. , GIBALDI, M. Biotechnology and Biopharmaceuticals. Transforming proteins and genes into drugs. John Willey and Sons, Hobokey, New Jersey, U.S.A, 2003. info
• SLATER, A., SCOTT, N., FOWLER, M. Plant biotechnology. The genetic manipulation of plants. Oxford University Press. Oxford, New York, U.S.A, 2003. info

recommended literature
• Alberts et al. Essential cell biology. Garland Science, Taylor and Francis Group. ISBN 0-8153-3481-8. info

not specified
• KAYSER, O., MÜLLER, R.H. Pharmaceutical Biotechnology. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim, Ge, 2004. info

Teaching methods (in Czech)

Monologická (výklad, přednáška, instruktáž)

Assessment methods (in Czech)

Ústní zkouška
Písemná zkouška

Language of instruction

English

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.

Teacher's information

Credits from practical course
Test: at least 50 % of credits
Oral examination

Activity	Difficulty [h]
Domácí příprava na výuku	12
Příprava na zkoušku	24
Účast na výuce	36
Summary	72

• Enrolment Statistics (Spring 2020, recent)
  
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