B5491 Molecular Biology - Practice 1

Faculty of Science
Autumn 1999
Extent and Intensity
0/8/0. 8 credit(s). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
prof. RNDr. Jan Šmarda, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites (in Czech)
( B2050 General microbiology || B4070 Obecná mikrobiologie || B4090 General microbiology ) && ( B4020 Molecular biology || B4030 Molecular biology || B6140 Embryology )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Syllabus
  • Preparation of solutions used in molecular biology. Isolation spontaneous mutants of Staphylococcus aureus resistant to streptomycine. One step growth curve of bacteriophage. Fluctuation test. Replica-plating of colonies a plaques. Isolation of plasmid DNA; Determination purity and concentration of DNA; Digestion of DNA by restriction endonucleases; Horizontal slab gel electrophoresis of DNA in agarose gels; Cultivation of eucaryotic cell cultures. Cytocentrifugation of influenced eucaryotic cells; Isolation of proteins; SDS-page methods.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 2000, Autumn 2001.
  • Enrolment Statistics (Autumn 1999, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn1999/B5491