PřF:Bi1050 Biological techniques - Course Information

Bi1050 The Study of Animal and Plant Structure for Biology Teachers

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Jaroslava Dubová, CSc. (seminar tutor)
RNDr. Božena Koubková, Ph.D. (seminar tutor)

Guaranteed by

RNDr. Jaroslava Dubová, CSc.
Biology Section – Faculty of Science
Contact Person: Eliška Karasová

Timetable of Seminar Groups

Bi1050/01: Tue 12:00–13:50 BpeR, Tue 12:00–13:50 BprR, J. Dubová, B. Koubková
Bi1050/02: Tue 14:00–15:50 BpeR, Tue 14:00–15:50 BprR, J. Dubová, B. Koubková
Bi1050/03: Tue 16:00–17:50 BpeR, Tue 16:00–17:50 BprR, J. Dubová, B. Koubková

Prerequisites (in Czech)

! Bi2302 Zoology microtechnique && ! Bi1301 Microscopic Techniques

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Biology with a view to Education (programme PřF, B-BI)

Course objectives

Introduction to the Light Microscope Structure and Function, Techniques of Preparations. Botanical Techniques: Replicas. Cleared Preparations. Squash Preparations. Histology and Histochemistry. Phase Contrast. Documentation. Zoological Techniques: Native Preparations, Vital Staining. Whole Mounts, Sections, Squashes, Dry Mounts. Fixation. Staining. Mounting Media.

Syllabus

• Introduction. Light Microscope: Structure and Function. Köhler Illumination. Botanical Techniques: Replicas for Study by Light Microscope, Stoma Structure and Position, Subsidiary cells. Whole Mounts of Cleared Preparations, Development of Angiosperm Embryos. Squash Preparations: Cell Cycle, Mitosis, Chromosome Counting, Chromosome Doubling. Anatomical Methods: Sectioning (Hand Sections, Fixation, Infiltration and Embedding, Microtome Sections), Staining. Phase Contrast. Documentation. Zoological Techniques: Native Preparations. Vital Staining. Whole Mounts, Sections, Squashes, Dry Mounts. Fixation. Fixatives. Staining. Aqueous Media. Non-aqueous Mounting Media.

Literature

• KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
• PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
• LELLÁKOVÁ, Františka. Zoologická technika. 1. vyd. Praha: Universita Karlova, 1985, 122 s. info
• NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
• BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info

Assessment methods (in Czech)

kurz je ukončen zápočtem, v botanické části jsou vyžadovány protokoly v zoologické části zhotovení trvalých preparátů

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• Bi2302 Zoological microtechnique .
  (!Bi1050) && (!NOW(Bi1050))  
• Bi4170 Optical contrast and imaging methods
  Bi1050||Bi2302||Bi1301  

• Enrolment Statistics (Autumn 2006, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2006/Bi1050