PřF:Bi1700c Cell Biology - practice - Course Information

Bi1700c Cell Biology - practical course

Extent and Intensity

0/1/0. 1 credit(s). Type of Completion: z (credit).
Taught in person.

Teacher(s)

MVDr. Mgr. Monika Dušková, Ph.D. (seminar tutor)
doc. RNDr. Jakub Neradil, Ph.D. (seminar tutor)
RNDr. Petr Chlapek, DiS., Ph.D. (seminar tutor)

Guaranteed by

doc. RNDr. Jakub Neradil, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: MVDr. Mgr. Monika Dušková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Timetable of Seminar Groups

Bi1700c/01_1: Tue 18:00–19:50 D36/215, M. Dušková
Bi1700c/01_2: Tue 18:00–19:50 D36/215, M. Dušková
Bi1700c/02_1: Wed 10:00–11:50 D36/215, M. Dušková
Bi1700c/02_2: Wed 10:00–11:50 D36/215, M. Dušková
Bi1700c/03_1: Wed 8:00–9:50 D36/215, M. Dušková
Bi1700c/03_2: Wed 8:00–9:50 D36/215, M. Dušková
Bi1700c/04_1: Tue 12:00–13:50 D36/209, P. Chlapek
Bi1700c/04_2: Tue 12:00–13:50 D36/209, P. Chlapek
Bi1700c/05_1: Wed 8:00–9:50 D36/209, J. Neradil
Bi1700c/05_2: Wed 8:00–9:50 D36/209, J. Neradil
Bi1700c/06_1: Wed 10:00–11:50 D36/209, J. Neradil
Bi1700c/06_2: Wed 10:00–11:50 D36/209, J. Neradil
Bi1700c/07_1: Wed 12:00–13:50 D36/215, J. Neradil
Bi1700c/07_2: Wed 12:00–13:50 D36/215, J. Neradil
Bi1700c/08_1: Wed 14:00–15:50 D36/215, P. Chlapek
Bi1700c/08_2: Wed 14:00–15:50 D36/215, P. Chlapek
Bi1700c/09_1: Wed 16:00–17:50 D36/215, P. Chlapek
Bi1700c/09_2: Wed 16:00–17:50 D36/215, P. Chlapek
Bi1700c/10_1: No timetable has been entered into IS. P. Chlapek

Prerequisites (in Czech)

NOW ( Bi1700 Cell Biology )

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

there are 26 fields of study the course is directly associated with, display

Course objectives

The practical course of Cell Biology consists of six thematic blocks of practical tasks, taking up the lecture of Cell Biology. The topics of the practical tasks involve issues such as the transport of substances across the cell membrane, cell locomotion and irritability, mitosis, meiosis, training with light microscope as well as basic laboratory procedures employed in biology laboratories. After this course, students should be able to work with automatic pipettes, to prepare solutions, to know the correct procedures for microscopy, and to prepare simple plant or animal microscopy slides. The course has been designed as a preparation for more specialized courses and is aimed to harmonize unequal levels of students' knowledge from secondary schools.

Learning outcomes

Graduating students will be able to apply automatic pipettes, preparation of solutions, evidence-based microscope procedures, sample (both plant and animal tissues) preparation; further the students will get the knowledge on cell morphology, osmotic changes, and on the cell division and movement

Syllabus

• 1. Measuring exact volumes in biology. Manipulation with automatic pipettes and pipetting heads or with glass pipettes: repeated (10 times) pipetting an exact volume of the liquid, weighing and statistical assessment of the precision of laboratory work. Model calculations of solution concentration 2. Manipulation with light microscope, training with immersion objective, measuring biological objects using a stage micrometer and a reticle. Native preparations of parenchyma cells from the Elodea waterweed, permanent slides of blood smears, measuring individual types of blood cells: erythrocytes, leukocytes, thrombocytes from humans and from other class of vertebrates. 3. Transport of substances, osmotic phenomena. Plasmolysis and deplasmolysis in epidermal cells of onion and the Elodea waterweed, dependence of reversibility of this process on the concentration of plasmolytic solution. Observation of pollen grains in hypotonic solution, reaction of erythrocytes in hyper- and hypotonic solutions. 4. Mitosis, modification of mitosis (polyploidy, polyteny). Mitotic figures in onion bulb cells, temporary and permanent slides, staining with aceto-orcein. Mitotic failures – onion bulb after hydrochinon treatment, temporary slide stained with aceto-orcein. Counting the mitotic index in permanent slides of tissue cultures. Preparation of salivary glands from Chironomidae, comparison of temporary and permanent slide, polyploid nuclei of the silkworm (Bombyx mori). 5. Meiosis. Permanent slides of the ovary and seminiferous tubules, egg cells from the Pelophylax frog, pigs' sperm cells – live spermatozoa and permanent slides. 6. Cell locomotion and irritability. Brownian motion observed in a suspension of ferric oxide particles. Observation of protozoa in a hay infusion – ciliary, amoeboid and flagellar motion. Chemotaxis of protozoa from a solution drop containing NaCl into a drop of pure water through a thin liquid bridge. Oxygenotaxis of protozoa around an air bubble.

Literature

• ALBERTS, Bruce. Základy buněčné biologie : úvod do molekulární biologie buňky. Translated by Arnošt Kotyk. 2. vyd. Ústí nad Labem: Espero Publishing. xxvi, 630. ISBN 8090290620. 2004. info

Teaching methods

laboratory practice, microscopic observation. The course will be taught full-time, in case of deterioration of the epidemiological situation will be the transition to online.

Assessment methods

Sstudents are regularly tested in course of practical exercise, each exercise requires written protocols prepared by attending students; the protocols are assessed by the teacher. Students who did not complete the continuous examination successfully are examined orally at the end of the course – in such case, all topics included in the course are examined.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on the extent and intensity of the course: každý druhý týden 2 hodiny.

Listed among pre-requisites of other courses

• LF:AMOLc Introduction into molecular biology and genetics - practice
  (!VSBI0222c) && (!VLBI0222c) && (!ZLBI0222c) && !NOWANY(VLBI0222c,ZLBI0222c) && (!PřF:Bi1700c)  
• Bi7001c Cell Architecture - Practicals
  Bi1700 && Bi1700c && NOW(Bi7001)  
• Bi8920 Advanced Microscopic Methods
  Bi1700 || Bi1700c  
• Bi8920c Fluorescence microscopy - practice
  Bi1700 || Bi1700c && NOW(Bi8920)  

• Enrolment Statistics (autumn 2021, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2021/Bi1700c