Bi7270 Plant Embryology
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cell Biology (programme PřF, B-EMB)
- Experimental Plant Biology (programme PřF, N-EBR)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- required literature
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- recommended literature
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- not specified
- PAVLOVÁ, Libuše and Lukáš FISCHER. Růst a vývoj rostlin. Vyd. 1. Praha: Karolinum, 2011, 325 s. ISBN 9788024619132. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations.
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2023
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 8:00–9:50 C13/332
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cell Biology (programme PřF, B-EMB)
- Experimental Plant Biology (programme PřF, N-EBR)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- required literature
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- recommended literature
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- not specified
- PAVLOVÁ, Libuše and Lukáš FISCHER. Růst a vývoj rostlin. Vyd. 1. Praha: Karolinum, 2011, 325 s. ISBN 9788024619132. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations.
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2022
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 14:00–15:50 C13/332
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cell Biology (programme PřF, B-EMB)
- Experimental Plant Biology (programme PřF, N-EBR)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- required literature
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- recommended literature
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- not specified
- PAVLOVÁ, Libuše and Lukáš FISCHER. Růst a vývoj rostlin. Vyd. 1. Praha: Karolinum, 2011, 325 s. ISBN 9788024619132. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of Scienceautumn 2021
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cell Biology (programme PřF, B-EMB)
- Experimental Plant Biology (programme PřF, N-EBR)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- required literature
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- recommended literature
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- not specified
- PAVLOVÁ, Libuše and Lukáš FISCHER. Růst a vývoj rostlin. Vyd. 1. Praha: Karolinum, 2011, 325 s. ISBN 9788024619132. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2019
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 9:00–10:50 D36/209
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Experimental Plant Biology (programme PřF, N-EBR)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- required literature
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- recommended literature
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- not specified
- PAVLOVÁ, Libuše and Lukáš FISCHER. Růst a vývoj rostlin. Vyd. 1. Praha: Karolinum, 2011, 325 s. ISBN 9788024619132. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2018
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 17. 9. to Fri 14. 12. Wed 9:00–10:50 D36/209
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of Scienceautumn 2017
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 18. 9. to Fri 15. 12. Wed 9:00–10:50 C13/332
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2016
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 19. 9. to Sun 18. 12. Wed 12:00–13:50 C13/332
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2015
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
RNDr. Jaroslava Dubová, CSc. (lecturer) - Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 12:00–13:50 C13/332
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2014
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Thu 10:00–11:50 C13/332
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2013
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant) - Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá - Timetable
- Mon 12:00–13:50 B11/235
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2009
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Věra Faronová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Věra Faronová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- This course continues to the course Bi1060 Plant Cytology and Anatomy and methodologically to the Bi1301 Microscopic Methods for the Study of Plant Structure. The main topics cover the development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation. Various methods of light microscopy (dark field, Nomarski DIC, fluorescence), different techniques for microscopic preparations as well as scanning electron microscopy are used for this study.
- Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Apical Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Assessment methods
- oral examination
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2007
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- This course continues to the course Bi1060 Plant Cytology and Anatomy and methodologically to the Bi1301 Microscopic Methods for the Study of Plant Structure. The main topics cover the development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation. Various methods of light microscopy (dark field, Nomarski DIC, fluorescence), different techniques for microscopic preparations as well as scanning electron microscopy are used for this study.
- Syllabus
- 1. Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy. 2. Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure. 3. Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems. 4. Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue, Flowering Initiation, Generative Meristem; Root Apical Meristem. 5. Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC. 6. Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures. 7. Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability. 8. Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube Phase Contract Microscopy and Nomarski DIC. 9. Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections. 10. Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections. 11. Pollination and Fertilization: Sporophyte Gametophyte interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis. 12. Embryo and Endosperm Development. 13. Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry. 14. Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Assessment methods (in Czech)
- ústní zkouška,
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- This course continues to the course Bi1060 Plant Cytology and Anatomy and methodologically to the Bi1301 Microscopic Methods for the Study of Plant Structure. The main topics cover the development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation. Various methods of light microscopy (dark field, Nomarski DIC, fluorescence), different techniques for microscopic preparations as well as scanning electron microscopy are used for this study.
- Syllabus
- 1. Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy. 2. Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure. 3. Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems. 4. Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue, Flowering Initiation, Generative Meristem; Root Apical Meristem. 5. Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC. 6. Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures. 7. Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability. 8. Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube Phase Contract Microscopy and Nomarski DIC. 9. Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections. 10. Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections. 11. Pollination and Fertilization: Sporophyte Gametophyte interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis. 12. Embryo and Endosperm Development. 13. Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry. 14. Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Assessment methods (in Czech)
- ústní zkouška,
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- This course continues to the course Bi1060 Plant Cytology and Anatomy and methodologically to the Bi1301 Microscopic Methods for the Study of Plant Structure. The main topics cover the development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation. Various methods of light microscopy (dark field, Nomarski DIC, fluorescence), different techniques for microscopic preparations as well as scanning electron microscopy are used for this study.
- Syllabus
- 1. Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy. 2. Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure. 3. Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems. 4. Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue, Flowering Initiation, Generative Meristem; Root Apical Meristem. 5. Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC. 6. Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures. 7. Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability. 8. Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube Phase Contract Microscopy and Nomarski DIC. 9. Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections. 10. Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections. 11. Pollination and Fertilization: Sporophyte Gametophyte interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis. 12. Embryo and Endosperm Development. 13. Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry. 14. Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Assessment methods (in Czech)
- ústní zkouška,
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2004
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 5 student(s).
Current registration and enrolment status: enrolled: 0/5, only registered: 0/5, only registered with preference (fields directly associated with the programme): 0/5 - fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- Light Microscope Construction and Function (Bright Field, Phase Contract Microscopy, Interference-DIC). Replicas for the Study of Surface. Rapid Cytological Methods: Cell Cycle, Nuclear Staining. Embryology: Microsporogenesis, Pollen Grain Development and Structure, Megasporogenesis, Embryo Sac,Fertilisation, Zygote, Embryo and Endosperm Development, Whole Mounts of Cleared Preparations. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. Cryosections and Histochemistry. Fluorescence Microscopy. Documentation.
- Syllabus
- 1. Development of Light Microscope Construction and Function (Bright Field, Köhler Illumination). 2. Replicas for the Study of Surface, Epidermis, Stoma Cells, Subsidiary Cells, Types of Stoma Structure. 3. Rapid Cytological Methods: Meristems, Cell Cycle, Cytoskeleton Organization during Cell Cycle, Nuclear Staining (Lacto-propionic Orcein, Cajal-Brožek Method), Pre-treatment. 4. Androeceum: Stamen, Development and Structure of Anther, Microsporogenesis, Pollen Grain Development and Structure, Pollen Grain Germination, Pollen Grain Viability. 5. Gynaecium: Whole Mounts of Cleared Ovules and Seeds, Megasporogenesis, Embryo and Endosperm Development. Seed Structure. 6. Phase Contract Microscopy, Nomarski DIC. 7. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. 8. Cryosections and Enzyme Histochemistry. 9. Fluorescence Microscopy, Autofluorescence, Fluorochromes, Immunofluorescence. 10. Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2003
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 5 student(s).
Current registration and enrolment status: enrolled: 0/5, only registered: 0/5, only registered with preference (fields directly associated with the programme): 0/5 - fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- Light Microscope Construction and Function (Bright Field, Phase Contract Microscopy, Interference-DIC). Replicas for the Study of Surface. Rapid Cytological Methods: Cell Cycle, Nuclear Staining. Embryology: Microsporogenesis, Pollen Grain Development and Structure, Megasporogenesis, Embryo Sac,Fertilisation, Zygote, Embryo and Endosperm Development, Whole Mounts of Cleared Preparations. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. Cryosections and Histochemistry. Fluorescence Microscopy. Documentation.
- Syllabus
- 1. Development of Light Microscope Construction and Function (Bright Field, Köhler Illumination). 2. Replicas for the Study of Surface, Epidermis, Stoma Cells, Subsidiary Cells, Types of Stoma Structure. 3. Rapid Cytological Methods: Meristems, Cell Cycle, Cytoskeleton Organization during Cell Cycle, Nuclear Staining (Lacto-propionic Orcein, Cajal-Brožek Method), Pre-treatment. 4. Androeceum: Stamen, Development and Structure of Anther, Microsporogenesis, Pollen Grain Development and Structure, Pollen Grain Germination, Pollen Grain Viability. 5. Gynaecium: Whole Mounts of Cleared Ovules and Seeds, Megasporogenesis, Embryo and Endosperm Development. Seed Structure. 6. Phase Contract Microscopy, Nomarski DIC. 7. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. 8. Cryosections and Enzyme Histochemistry. 9. Fluorescence Microscopy, Autofluorescence, Fluorochromes, Immunofluorescence. 10. Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2002
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 5 student(s).
Current registration and enrolment status: enrolled: 0/5, only registered: 0/5, only registered with preference (fields directly associated with the programme): 0/5 - fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- Light Microscope Construction and Function (Bright Field, Phase Contract Microscopy, Interference-DIC). Replicas for the Study of Surface. Rapid Cytological Methods: Cell Cycle, Nuclear Staining. Embryology: Microsporogenesis, Pollen Grain Development and Structure, Megasporogenesis, Embryo Sac,Fertilisation, Zygote, Embryo and Endosperm Development, Whole Mounts of Cleared Preparations. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. Cryosections and Histochemistry. Fluorescence Microscopy. Documentation.
- Syllabus
- 1. Development of Light Microscope Construction and Function (Bright Field, Köhler Illumination). 2. Replicas for the Study of Surface, Epidermis, Stoma Cells, Subsidiary Cells, Types of Stoma Structure. 3. Rapid Cytological Methods: Meristems, Cell Cycle, Cytoskeleton Organization during Cell Cycle, Nuclear Staining (Lacto-propionic Orcein, Cajal-Brožek Method), Pre-treatment. 4. Androeceum: Stamen, Development and Structure of Anther, Microsporogenesis, Pollen Grain Development and Structure, Pollen Grain Germination, Pollen Grain Viability. 5. Gynaecium: Whole Mounts of Cleared Ovules and Seeds, Megasporogenesis, Embryo and Endosperm Development. Seed Structure. 6. Phase Contract Microscopy, Nomarski DIC. 7. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. 8. Cryosections and Enzyme Histochemistry. 9. Fluorescence Microscopy, Autofluorescence, Fluorochromes, Immunofluorescence. 10. Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2020
The course is not taught in Autumn 2020
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (lecturer)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Experimental Plant Biology (programme PřF, N-EBR)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Learning outcomes
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- required literature
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- recommended literature
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Plant cell morphogenesis : methods and protocols. Edited by Viktor Žárský - Fatima Cvrčková. New York: Springer, 2014, xi, 285. ISBN 9781627036429. info
- not specified
- PAVLOVÁ, Libuše and Lukáš FISCHER. Růst a vývoj rostlin. Vyd. 1. Praha: Karolinum, 2011, 325 s. ISBN 9788024619132. info
- Teaching methods
- Lectures with Microsoft PowerPoint presentations, microkinematography
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Embryology
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Věra Faronová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- At the end of this course, students should be able to:
describe development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation;
utilize various methods of light microscopy (dark field, Nomarski DIC, fluorescence microscopy) as well as different techniques of microscopic preparations, even for scanning electron microscopy. - Syllabus
- Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy.
- Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure.
- Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems.
- Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue), Flowering Initiation, Generative Meristem; Root Meristem.
- Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC.
- Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability.
- Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube, Phase Contract Microscopy and Nomarski DIC.
- Pollination and Fertilization: Sporophyte Gametophyte Interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis.
- Embryo and Endosperm Development, Embryogenetic Types, Types of Endosperm Development.
- Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures.
- Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections.
- Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections.
- Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry.
- Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- recommended literature
- VINTER, Vladimír. Rostliny pod mikroskopem. 2nd ed. Olomouc, 2009, 200 pp. ISBN 978-80-244-2223-7. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Teaching methods
- Lectures
- Assessment methods
- Oral examination
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
Bi7270 Plant Cytology and Anatomy for Advanced Students
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- RNDr. Jaroslava Dubová, CSc. (lecturer)
- Guaranteed by
- RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- This course continues to the course Bi1060 Plant Cytology and Anatomy and methodologically to the Bi1301 Microscopic Methods for the Study of Plant Structure. The main topics cover the development and structure of plant generative organs, formation of gametes, structural aspects of the pollination and fertilization process, embryo and endosperm formation. Various methods of light microscopy (dark field, Nomarski DIC, fluorescence), different techniques for microscopic preparations as well as scanning electron microscopy are used for this study.
- Syllabus
- 1. Development of Light Microscope Construction and Function: Bright Field, Köhler Illumination and Ten Commandments in Light Microscopy. 2. Plant Life Cycles: Sporophyte, Gametophyte, Fertilization, Zygote, Embryo, Seed, Structure of Angiosperm Seeds, Seed Quiescence and Dormancy, Seed Germination and Seedling Structure. 3. Cell Nucleus and its Division: Phases of the Cell Cycle, Cell Cycle Control Points, Cytoskeleton Organization during Cell Cycle, Nuclear Staining, Rapid Cytological Methods (Lacto-Propionic Orcein, Cajal-Brožek Method), Pre-treatment, Classification of Meristems. 4. Primary Meristems: Stem Apical Meristem (Structure, Stem Cells, Vegetative Meristem, Leaf Primordia, Leaf Meristems, Differentiation of Leaf Tissue, Flowering Initiation, Generative Meristem; Root Apical Meristem. 5. Megasporogenesis and Megagametogenesis: Gynoecium, Pistil Development and Structure, Structure of Angiosperm Ovule, Meiosis, Tetrads of Megaspores, Embryo Sac Development, Types of Embryo Sac, Whole Mounts of Cleared Ovules and Seeds, Nomarski DIC. 6. Histological Techniques in Plant Embryology I.: Fixation and Fixatives and their Mixtures. 7. Microsporogenesis and Microgametogenesis: Androecium: Stamen, Anther Structure and Development, Meiosis, Tetrads of Microspores, Pollen Grain Development and Maturation, Pollen Wall Structure, Pollen Grain Viability. 8. Pollen Grain Germination in situ and in vitro: Incompatibility, Pollen Tube Phase Contract Microscopy and Nomarski DIC. 9. Histological Techniques in Plant Embryology II: Dehydration, Infiltration and Embedding, Microtome Types, Sectioning, Adhesion of Sections. 10. Histological Techniques in Plant Embryology II: Dyes and Staining, Embedding of Sections. 11. Pollination and Fertilization: Sporophyte Gametophyte interactions, Growth of Pollen Tubes and Fluorescence Microscopy, Decolourised Aniline Blue, Callose staining, Callose Plugs, Sperm Cell Formation, Double Fertilization in Angiosperms, Apomixis. 12. Embryo and Endosperm Development. 13. Cryostat Techniques in Embryology: Fixation, Cryoprotection and Cryosections, Enzyme Histochemistry. 14. Documentation: Photomicrography, Video, and Distance Measurements.
- Literature
- PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
- NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
- ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
- PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
- ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
- RAGHAVAN, V. Developmental biology of flowering plants. New York: Springer Verlag, 1999, xxii, 354. ISBN 0387987819. info
- RAGHAVAN, Valayamghat. Molecular embryology of flowering plants. 1st pub. Cambridge: Cambridge University Press, 1997, xxi, 690 s. ISBN 0-521-55246-X. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
- KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
- Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
- HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
- Assessment methods (in Czech)
- ústní zkouška,
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Enrolment Statistics (Autumn 2024, recent)