Bi8141 Molecular Physiology of Genom

Faculty of Science
Spring 2009
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Stanislav Kozubek, DrSc. (lecturer)
doc. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 10:00–11:50 BFU
Prerequisites
Lectures are prepared for students that are interested in chromatin biology.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The main aims of lectures from "Molecular Physiology of the Genome" lead to understanding of correlations between structure and function of chromatin. Lectures offer new knowledge in the field of chromatin biology. Arrangement of higher order chromatin structure is discussed from the view of physiological events such as cell proliferation, differentiation and cell death apoptosis. Moreover, we are interested in changes in chromatin architecture during pathophysiological process such as tumor cell transformation. A part of chromatin structure course pays an attention to epigenetic regulation of genome. Students are informed about functional and structural aspects of histone acetylation, histone methylation and DNA methylation. In the next part of course we lecture latest principles and facilities of confocal microscopy and high-resolution cytometry. Students are familiarized with a vast array of molecular-biology methods. At the and of course the student will be able to explain an importance of chromatin structure during regulation of replication, transcription, splicing, and DNA reparation. Students would understand the principles of important methods such as fluorescence in situ hybridization (FISH), chromatin immunoprecipitation, comparative genomic hybridization (CGH), imunofluorescence, RT-PCR, and others. Students gain a lot of knowledge in the filed of tumor cell biology, which could applied in other courses and their diploma thesis with similar field of interest. The chromatin biology is explained from the view of basic research and students are familiarized with recent trends in this attractive filed of science.
Syllabus
  • 1. Cell nucleus, nucleolus – compartmentalization of interphase nuclei
  • 2. Transcription, RNA processes and RNA interference
  • 3. Structure of chromatin and transcription
  • 4. Structure of chromatin and cell differentiation
  • 5. Structure of chromatin and apoptosis
  • 6. Epigenetic aspects of chromatin
  • 7. Cytoskeleton, lamins and controversial nuclear matrix
  • 8. BAC/PAC libraries and DNA probe preparation for FISH technique
  • 9. Confocal microscopy and related methods
  • 10. Structure of chromatin and epigenetic changes in tumor cells
  • 11. High resolution cytometry – principles, statistic and bioinformatic analysis
  • 12. Microarray technology
  • 13. DNA reparation mechanisms
  • 14. Credit week - J.G. Mendel and his importance, visit of Mendelianum
  • Visit - http://www.ibp.cz/labs/LMCC/publications.php
Literature
Assessment methods
Oral exam, each student prepares analysis of scientific paper in selected field of science or recommended by tutor. Courses must be attended. Tutor’s presentation will be provided in pdf format.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
http://www.ibp.cz/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2016, spring 2018, Spring 2019, Spring 2021, Spring 2024.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2009/Bi8141