Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching - Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- Mgr. Karel Souček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 14:00–15:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Learning outcomes
- The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Practical Flow Cytometry
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2023
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Hana Polášek-Sedláčková, PhD. (lecturer) - Guaranteed by
- Mgr. Karel Souček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 17:00–18:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Learning outcomes
- The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Practical Flow Cytometry
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2022
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Soňa Legartová, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer) - Guaranteed by
- prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 14:00–15:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Learning outcomes
- The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Practical Flow Cytometry
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of Scienceautumn 2021
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Soňa Legartová, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer) - Guaranteed by
- prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 14:00–15:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Learning outcomes
- The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Practical Flow Cytometry
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2020
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Soňa Legartová, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer) - Guaranteed by
- prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 14:00–15:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Learning outcomes
- The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Practical Flow Cytometry
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2019
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer) - Guaranteed by
- prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 14:00–15:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Learning outcomes
- The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Practical Flow Cytometry
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2018
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Radek Fedr (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 17. 9. to Fri 14. 12. Tue 14:00–15:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Practical Flow Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of Scienceautumn 2017
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Radek Fedr (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 18. 9. to Fri 15. 12. Tue 16:00–17:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Practical Flow Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2016
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 19. 9. to Sun 18. 12. Tue 16:00–17:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Practical Flow Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2015
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 16:00–17:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Practical Flow Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2014
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 16:00–17:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- Flow Cytometry: Instrumentation and Data Analysis
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Practical Flow Cytometry
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2013
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 16:00–17:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- recommended literature
- - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
- Practical Flow Cytometry
- Flow Cytometry: Instrumentation and Data Analysis
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 16:00–17:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu! - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Timetable
- Tue 16:00–17:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Timetable
- Tue 17:00–18:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2009
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Timetable
- Tue 17:00–18:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Timetable
- Tue 17:00–18:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2007
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Timetable
- Tue 17:00–18:50 BFU
- Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research
- Assessment methods (in Czech)
- Kurz bude postaven zejména na přednáškách a diskuzích k nim. V rámci přednášek budou objasněny principy a způsoby základní analýzy dat k jednotlivým metodologiím. Studenti formou instruktáže seznámeni s vybavením pro průtokovou cytometrii a digitální mikroskopii které je v provozu na Biofyzikálním ústavu v Brně. Zkouška Každý student bude muset prezentovat krátký seminář jehož téma bude konzultováno s přednášejícím a bude se týkat zaměření kurzu. Na základě této prezentace bude udělen zápočet a hodnocení vlastního semináře se bude také z 30-ti % odrážet v celkové známce. Kurz bude zakončen zkouškou ve formě testu shrnujícího látku za celý semestr. Výsledek testu bude tvořit 70% celkového hodnocení.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Timetable
- Tue 17:00–18:50 BFU
- Prerequisites
- Any limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research
- Assessment methods (in Czech)
- Kurz bude postaven zejména na přednáškách a diskuzích k nim. V rámci přednášek budou objasněny principy a způsoby základní analýzy dat k jednotlivým metodologiím. Studenti formou instruktáže seznámeni s vybavením pro průtokovou cytometrii a digitální mikroskopii které je v provozu na Biofyzikálním ústavu v Brně. Zkouška Každý student bude muset prezentovat krátký seminář jehož téma bude konzultováno s přednášejícím a bude se týkat zaměření kurzu. Na základě této prezentace bude udělen zápočet a hodnocení vlastního semináře se bude také z 30-ti % odrážet v celkové známce. Kurz bude zakončen zkouškou ve formě testu shrnujícího látku za celý semestr. Výsledek testu bude tvořit 70% celkového hodnocení.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- Flow cytometric methods in cell death analysis (Alena Vaculova)
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
- Literature
- Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
- Teaching methods
- The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
- Assessment methods
- Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
Bi9393 Analytical cytometry
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D. - Prerequisites
- No limitation for enrollment.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Physiology of Animals)
- Course objectives
- This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
- Syllabus
- * Flow cytometry general introduction - basic principles and history (Karel Souček)
- * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
- * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
- * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
- * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
- * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
- * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
- * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research
- Assessment methods (in Czech)
- Kurz bude postaven zejména na přednáškách a diskuzích k nim. V rámci přednášek budou objasněny principy a způsoby základní analýzy dat k jednotlivým metodologiím. Studenti formou instruktáže seznámeni s vybavením pro průtokovou cytometrii a digitální mikroskopii které je v provozu na Biofyzikálním ústavu v Brně. Zkouška Každý student bude muset prezentovat krátký seminář jehož téma bude konzultováno s přednášejícím a bude se týkat zaměření kurzu. Na základě této prezentace bude udělen zápočet a hodnocení vlastního semináře se bude také z 30-ti % odrážet v celkové známce. Kurz bude zakončen zkouškou ve formě testu shrnujícího látku za celý semestr. Výsledek testu bude tvořit 70% celkového hodnocení.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi9393en Analytical Cytometry
(!Bi9393) && !NOWANY(Bi9393)
- Bi9393en Analytical Cytometry
- Enrolment Statistics (recent)