Bi9393 Analytical cytometry

Faculty of Science
Autumn 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
Mgr. Karel Souček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 14:00–15:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Hana Polášek-Sedláčková, PhD. (lecturer)
Guaranteed by
Mgr. Karel Souček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17:00–18:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Soňa Legartová, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 14:00–15:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Soňa Legartová, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 14:00–15:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2020
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Soňa Legartová, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 14:00–15:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 14:00–15:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2018
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Radek Fedr (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Tue 14:00–15:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
  • Practical Flow Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
autumn 2017
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Mgr. Radek Fedr (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Tue 16:00–17:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
  • Practical Flow Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2016
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 9. to Sun 18. 12. Tue 16:00–17:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
  • Practical Flow Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2015
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 16:00–17:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
  • Practical Flow Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2014
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 16:00–17:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
  • Practical Flow Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2013
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 16:00–17:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
    recommended literature
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2012
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 16:00–17:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
  • Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2011
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Timetable
Tue 16:00–17:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
  • Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2010
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Timetable
Tue 17:00–18:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
  • Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2009
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Timetable
Tue 17:00–18:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
  • Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2008
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Timetable
Tue 17:00–18:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
  • Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2007
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Timetable
Tue 17:00–18:50 BFU
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research
Assessment methods (in Czech)
Kurz bude postaven zejména na přednáškách a diskuzích k nim. V rámci přednášek budou objasněny principy a způsoby základní analýzy dat k jednotlivým metodologiím. Studenti formou instruktáže seznámeni s vybavením pro průtokovou cytometrii a digitální mikroskopii které je v provozu na Biofyzikálním ústavu v Brně. Zkouška Každý student bude muset prezentovat krátký seminář jehož téma bude konzultováno s přednášejícím a bude se týkat zaměření kurzu. Na základě této prezentace bude udělen zápočet a hodnocení vlastního semináře se bude také z 30-ti % odrážet v celkové známce. Kurz bude zakončen zkouškou ve formě testu shrnujícího látku za celý semestr. Výsledek testu bude tvořit 70% celkového hodnocení.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2006
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Timetable
Tue 17:00–18:50 BFU
Prerequisites
Any limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research
Assessment methods (in Czech)
Kurz bude postaven zejména na přednáškách a diskuzích k nim. V rámci přednášek budou objasněny principy a způsoby základní analýzy dat k jednotlivým metodologiím. Studenti formou instruktáže seznámeni s vybavením pro průtokovou cytometrii a digitální mikroskopii které je v provozu na Biofyzikálním ústavu v Brně. Zkouška Každý student bude muset prezentovat krátký seminář jehož téma bude konzultováno s přednášejícím a bude se týkat zaměření kurzu. Na základě této prezentace bude udělen zápočet a hodnocení vlastního semináře se bude také z 30-ti % odrážet v celkové známce. Kurz bude zakončen zkouškou ve formě testu shrnujícího látku za celý semestr. Výsledek testu bude tvořit 70% celkového hodnocení.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
  • Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
Literature
  • Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

Bi9393 Analytical cytometry

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Prerequisites
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
Course objectives
This course will introduce principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays. Analysis of data and their interpretation and presentation will be explained in details. Part of the course is also practical demonstration of selected equipments and methods of data analysis. We will put stress on possibilities and methods for practical applications in medicine and biology.
Syllabus
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, flow cytometric methods in cell death analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research
Assessment methods (in Czech)
Kurz bude postaven zejména na přednáškách a diskuzích k nim. V rámci přednášek budou objasněny principy a způsoby základní analýzy dat k jednotlivým metodologiím. Studenti formou instruktáže seznámeni s vybavením pro průtokovou cytometrii a digitální mikroskopii které je v provozu na Biofyzikálním ústavu v Brně. Zkouška Každý student bude muset prezentovat krátký seminář jehož téma bude konzultováno s přednášejícím a bude se týkat zaměření kurzu. Na základě této prezentace bude udělen zápočet a hodnocení vlastního semináře se bude také z 30-ti % odrážet v celkové známce. Kurz bude zakončen zkouškou ve formě testu shrnujícího látku za celý semestr. Výsledek testu bude tvořit 70% celkového hodnocení.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (recent)