C2135 Bioinformatika v praxi

Faculty of Science
Spring 2012
Extent and Intensity
0/2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
prof. RNDr. Michaela Wimmerová, Ph.D. (lecturer)
Mgr. Josef Houser, Ph.D. (seminar tutor)
Mgr. Lenka Malinovská, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Michaela Wimmerová, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Wed 11:00–12:50 C04/118
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to: search for information and work with biological databases; perform independent bioinformatical analyses of data; prepare experimental project focused to protein cloning
Syllabus
  • Information search. General search, biological databases, resources for molecular biology information
  • Sequence alignment. Parameters (gaps, matrix). Nucleotide vs. amino acid alignments. Repetitions. Alignment as a tool for sequencing data analyses.
  • Gene prediction, prediction of proteinsand their functions.
  • Primer design. Their importance, basic characteristics, secondary structure prediction, false priming, design of specific primers.
  • Cloning and restriction endonucleases digestion.
  • Protein properties prediction. Possibilities, comparison of demands and reliability of calculation and experiment.
  • Individual project: identification of putative genes in the genome of an unknown organism, which are important for pro virulence. Function prediction of encoded proteins.
  • Practical exercise 1. Polymeráse chain reaction and agarose elektrophoresis
  • Practical exercise 2. PCR product isolation, digestion using restriction endonucleases.
Literature
Teaching methods
homework, practical exercises in silico, individual project
Assessment methods
own project finished, oral discussion
Language of instruction
Czech
Further Comments
Study Materials
Course is no more offered.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (Spring 2012, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2012/C2135