PřF:C3600 Biochemistry-lab.course - Course Information

C3600 Biochemistry - laboratory course

Extent and Intensity

0/5/0. 5 credit(s). Type of Completion: z (credit).
Taught in person.

Teacher(s)

Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Lukáš Faltinek (assistant)
Mgr. Tereza Hlaváčová (assistant)
Daniil Kabanov (assistant)
Ing. Jakub Máčala (assistant)
Mgr. Radka Obořilová (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3600/01: Mon 7:00–11:50 C15/212, Z. Farka, R. Obořilová
C3600/02: Wed 8:00–12:50 C15/212, L. Faltinek, M. Pelcová
C3600/03: Thu 8:00–12:50 C15/212, K. Dadáková, D. Kabanov
C3600/04: Thu 13:00–17:50 C15/212, J. Máčala, V. Sedláček
C3600/05: Fri 8:00–12:50 C15/212, T. Hlaváčová, J. Kučera

Prerequisites (in Czech)

NOW ( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 10 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing. S. II-XIV,. ISBN 80-85605-44-9. 1995. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons. 1 sv. ISBN 0471586501. 1999. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

• Enrolment Statistics (autumn 2021, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2021/C3600