C5900 Mass Spectrometry

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
PharmDr. Zdeněk Spáčil, Ph.D. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Tue 14:00–15:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the context with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization techniques, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization techniques and fragmentation processes;
- asses and interpret the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization techniques;
- evaluate significance of connection of mass spectrometry with various analytical techniques, especially with separation techniques GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroscopy in quantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tuning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantification in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017.

C5900 Mass Spectrometry

Faculty of Science
autumn 2017
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Tue 14:00–15:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the context with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization techniques, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization techniques and fragmentation processes;
- asses and interpret the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization techniques;
- evaluate significance of connection of mass spectrometry with various analytical techniques, especially with separation techniques GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroscopy in quantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tuning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantification in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2016
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Mon 19. 9. to Sun 18. 12. Tue 14:00–15:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the context with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization techniques, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization techniques and fragmentation processes;
- asses and interpret the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization techniques;
- evaluate significance of connection of mass spectrometry with various analytical techniques, especially with separation techniques GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroscopy in quantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tuning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantification in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2015
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 14:00–15:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the context with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization techniques, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization techniques and fragmentation processes;
- asses and interpret the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization techniques;
- evaluate significance of connection of mass spectrometry with various analytical techniques, especially with separation techniques GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroscopy in quantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tuning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantification in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2014
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 14:00–15:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the context with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization techniques, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization techniques and fragmentation processes;
- asses and interpret the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization techniques;
- evaluate significance of connection of mass spectrometry with various analytical techniques, especially with separation techniques GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroscopy in quantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tuning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantification in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2013
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 15:00–16:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the context with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization techniques, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization techniques and fragmentation processes;
- asses and interpret the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization techniques;
- evaluate significance of connection of mass spectrometry with various analytical techniques, especially with separation techniques GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroscopy in quantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tuning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantification in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2012
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
Mgr. Petr Kukučka, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX – Faculty of Science
Timetable
Tue 15:00–16:50 D29/252-RCX1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the contenxt with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization technics, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization technics and fragmentation processes;
- asses and inerprete the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization technics;
- evaluate significance of connection of mass spectrometry with various analytical technics, especially with separation technics GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroskopy in kvantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2011
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
Mgr. Petr Kukučka, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Tue 15:00–16:50 409-stara KAM1
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the contenxt with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization technics, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization technics and fragmentation processes;
- asses and inerprete the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization technics;
- evaluate significance of connection of mass spectrometry with various analytical technics, especially with separation technics GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroskopy in kvantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2010
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jiří Machát, Ph.D. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 13:00–14:50 B11/114
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the contenxt with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization technics, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization technics and fragmentation processes;
- asses and inerprete the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization technics;
- evaluate significance of connection of mass spectrometry with various analytical technics, especially with separation technics GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroskopy in kvantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2009
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jiří Machát, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 13:00–14:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the contenxt with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization technics, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization technics and fragmentation processes;
- asses and inerprete the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization technics;
- evaluate significance of connection of mass spectrometry with various analytical technics, especially with separation technics GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroskopy in kvantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jiří Machát, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Thu 7:00–8:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented. Mail goals of this course: to obtain a survey about development and current state of methods and instrumentation of mass spectrometry; to use of MS for analysis of inorganic and organic compounds in the various type of samples.
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods
lectures, oral exam
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2007
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: RNDr. Miroslav Ciganek, Ph.D.
Timetable
Tue 13:00–14:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented.
Syllabus
  • I. History, principles of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2006
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (alternate examiner)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: RNDr. Miroslav Ciganek, Ph.D.
Timetable
Fri 9:00–10:50 Kontaktujte učitele
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented.
Syllabus
  • I. History, principles of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2005
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
Chemistry Section – Faculty of Science
Contact Person: RNDr. Miroslav Ciganek, Ph.D.
Timetable
Mon 13:00–14:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented.
Syllabus
  • I. History, principles of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2004
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
Chemistry Section – Faculty of Science
Contact Person: RNDr. Miroslav Ciganek, Ph.D.
Timetable
Fri 14:00–15:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented.
Syllabus
  • I. History, principles of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2003
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
Chemistry Section – Faculty of Science
Contact Person: RNDr. Miroslav Ciganek, Ph.D.
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented.
Syllabus
  • I. History, principles of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
RECETOX – Faculty of Science
Contact Person: prof. RNDr. Jakub Hofman, Ph.D.
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented.
Syllabus
  • I. History, principles of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2001
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
Guaranteed by
doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Prerequisites
Knowledge of Analytical chemistry on a level of introductory course
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques as GC/MS, HPLC/MS, CE/MS, and ICP/MS are presented.
Syllabus
  • I. History, principle of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2000
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
Guaranteed by
doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Prerequisites
Knowledge of Analytical chemistry on a level of introductory course
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques as GC/MS, HPLC/MS, CE/MS, and ICP/MS are presented.
Syllabus
  • I. History, principle of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 1999
Extent and Intensity
2/0/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
Guaranteed by
doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • I. Principles of MS II. Instrumentation: Sample introduction, Vacuum system, Ionization, Ion analysis. MS/MS and tandem MS. Collisional activation. Ion detection. III. Fragmentation: Metastable ions, methods of study. Nuclide ions, ion cluster. Fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation. V. Hyphenation of analytical techniques and MS: Data acquisition and analysis - repetitive scanning, TIC, mass and SIM chromatograms. GC/MS, HPLC/MS, CE/MS, ICP/MS.
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jan Kuta, Ph.D. (lecturer)
Mgr. Petr Kukučka, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the contenxt with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization technics, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization technics and fragmentation processes;
- asses and inerprete the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization technics;
- evaluate significance of connection of mass spectrometry with various analytical technics, especially with separation technics GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroskopy in kvantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jana Klánová, Ph.D. (lecturer)
Mgr. Jiří Machát, Ph.D. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students should be able to:
- define fundamentals of mass spectrometry and describe it in the contenxt with other spectral analytical methods;
- understand and clarify principles of instrumentation and functional layout of current ionization technics, mass analyzers and detecting elements;
- explain the mechanisms of ion fragmentation and dissociation using current ionization technics and fragmentation processes;
- asses and inerprete the mass spectra of common organic and anorganic compounds obtained by most frequently used ionization technics;
- evaluate significance of connection of mass spectrometry with various analytical technics, especially with separation technics GC/MS, HPLC/MS, CE/MS, ICP/MS;
- take advantage of mass spectroskopy in kvantitative and qualitative analyses of various types of samples;
Syllabus
  • I. History, principles of MS, fundamental terms.
  • II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning.
  • III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms.
  • IV. Mass spectra and their use. Quantitation in MS.
  • V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Teaching methods
Education is performed as lectures with Powerpoint prezentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assesment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

C5900 Mass Spectrometry

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslav Ciganek, Ph.D. (lecturer)
Ing. Anton Kočan, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: RNDr. Miroslav Ciganek, Ph.D.
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory section treats in detail the contemporary instrumentation of mass spectrometry, principles of ionization, analysis and ion detection. Topics of ion fragmentation, compounds identification and quantitation follow. Finally, hyphenated techniques - GC/MS, HPLC/MS, CE/MS, and ICP/MS - are presented.
Syllabus
  • I. History, principles of MS, fundamental terms. II. Instrumentation. Sample introduction, vacuum system. Sample ionization, methods of ionization of volatiles / nonvolatiles compounds, soft and hard ionization techniques. Ion analysis, resolution, magnetic sector, electrostatic analysator, HRMS. TOF analysator and MALDI-TOF equipment. Ion cyclotron resonance. Linear quadrupole, ion trap. Tandem MS. Collisional activation. Ion detection. Spectrometer tunning. III. Fragmentation. Metastable ions, nuclide ions, fundamental fragmentation mechanisms. IV. Mass spectra and their use. Quantitation in MS. V. Hyphenated techniques. Hyphenation with separation techniques GC/MS, HPLC/MS, CE/MS. Data acquisition and analysis. ICP/MS technique.
Literature
  • BARKER, J. Mass Spectrometry. 2nd Ed. Cichester: J. Wiley, 1999. Analytical Chemistry by Open Learning. ISBN 0 471 96762 9. info
  • BOEHM, S. and S. SMRČKOVÁ. Strukturní analýza organických sloučenin (Structure analysis of organic compounds). Praha: VŠCHT Praha, 1995. ISBN 80-7080-235-9. info
  • DE HOFFMAN, E. Tandem Mass Spectrometry: A Primer. Journal of Mass Spectrometry. John Wiley & Sons, Ltd., 1996, vol. 31, p. 129-138. ISSN 1076-5174. info
  • WONG, P. S. H. and R. G. COOKS. Ion Trap Mass Spectrometry. Current Separations. West Lafayette, USA: Bioanalytical Systems, Inc., 1997, vol. 16, p. 85. info
  • MCLAFFERTY, F.W. and F. TUREČEK. Interpretation of Mass Spectra. 4th ed. Sausalito , CA: University Science Book, 1993. ISBN 0-935702-25-3. info
  • KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
Assessment methods (in Czech)
přednášky, ústní zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (recent)