C5910 Chromatographic Methods I.

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (assistant)
Guaranteed by
RECETOX – Faculty of Science
Contact Person: prof. RNDr. Jakub Hofman, Ph.D.
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Introductory part deals with chromatographic terms, chromatogram evaluation, and theory of chromatographic separation. Discussion of principles, techniques, instrumentation, and contemporary trends in the field of high performance liquid chromatography (HPLC) and high performance thin-layer chromatography (HPTLC) follows. Capillary electrophoresis (HPCE) and electrochromatography (CEC) as well as gas chromatography (HRGC), are discussed in Chromatographic Methods II course.
Syllabus
  • I. Chromatographic separation, chromatogram and its evaluation, separation efficiency, column performance. Band broadening. Literature. II. Liquid chromatography II.1 HPLC, Stationary and mobile phases. II.2 HPLC column, stationary phase, features of individual sorbents. II.3 Mobile phase, solvent classification, multicomponent mobile phases and optimization of their composition, elution techniques. II.4 HPLC techniques, principles, retention models, separation strategy, applications. II.5 HPLC instrumentation, solvent delivery, injection devices, detectors and detection principles. Derivatization techniques in HPLC. II.6 Methods of quantitative analysis II.7 Sample preparation. Multidimensional chromatography. Microcolumns and capillary columns. High speed HPLC. III. Thin Layer Chromatography. Comparison of TLC and HPTLC. HPTLC technique. Quantitation. Instrumentation. Applications.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • MEYER, Veronika R. Practical High-Performance Liquid Chromatography. 3rd ed. Chichester: J. Wiley & Sons, 1999, 338 pp. ISBN 0-471-98372-1. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Assessment methods (in Czech)
přednáška, ústní zkouška
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2002, recent)
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