Course Information

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught in person.

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught in person.

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught in person.

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught in person.

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught in person.

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 1. 3. to Fri 14. 5. Thu 10:00–11:50 online_BCH3

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 18. 2. to Fri 17. 5. Thu 10:00–11:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Thu 10:00–11:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 20. 2. to Mon 22. 5. Thu 10:00–11:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Wed 11:00–12:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Wed 13:00–14:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Wed 14:00–15:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 13:00–14:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

lectures, oral examination, alternatively colloquium

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Timetable

Thu 10:00–11:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

lectures, oral examination, alternatively colloquium

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Timetable

Wed 14:00–15:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

lectures, oral examination, alternatively colloquium

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Timetable

Wed 15:00–16:50 C05/114

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

lectures, oral examination, alternatively colloquium

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Timetable

Thu 13:00–14:50 C05/107

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Assessment methods

lectures, oral examination, alternatively colloquium

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Seminář ke cvičení C5985

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Dana Modráčková (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Příhoda, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science

Timetable

Tue 8:00–9:50 -1020

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Chemistry of Conservation - Restoration (programme PřF, B-CH)

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Seminář ke cvičení C5985

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Dana Modráčková (seminar tutor)

Guaranteed by

Mgr. Dana Modráčková
Department of Chemistry – Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Chemistry of Conservation - Restoration (programme PřF, B-CH)

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Initial information on enzymes, basic definitions, history. 2. Enzyme aktivity, units, methods of measurement, suitable substrates. 3. Isolation of enzymes, purification procedures, characterization. 4. Mechanisms of enzyme catalysis. 5. Kinetics of enzyme reaction with substrate, Michaelis-Menten equation, kinetic parameters, vmax (vlim), Km a vmax / Km. 5. Multisubstrate reactions, classification and kinetics, determination of mechanism. 6. Inhibition, reversible and irreversible inhibitors, methods of evaluation. 7. Software for enzyme kinetics. EnzLab, Gepasi. 8. Effect of external factors on enzyme reactions, pH, temperature, viscosity. 9. Cooperation effects, allosteric enzymes, Hill equation. 10. Imobilized enzymes, enzyme reactors. 11. Bioanalytical applications of enzymes. 12. Application of enzymes in immunochemical techniques. 13. Industrial applications of enzymes.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/2/0. 5 credit(s). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Syllabus

• An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2024

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2023

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2022

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in autumn 2021

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2020

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2019

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2018

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in autumn 2017

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Learning outcomes

Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2016

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2015

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The course is not taught in Autumn 2014

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.

Syllabus

• 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Seminář ke kurzu C5985

The course is not taught in Spring 2004

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Dana Modráčková (seminar tutor)

Guaranteed by

Mgr. Dana Modráčková
Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is offered to students of any study field.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

lectures, oral examination, alternatively colloquium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Applied enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. At its end students should understand this topics - Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Literature

• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info

Teaching methods

lecture course, finished by examination

Assessment methods

lectures, oral examination, alternatively colloquium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Seminář ke cvičení C5985

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).

Teacher(s)

ak. mal. Zdenka Kuželová (seminar tutor)
Mgr. Dana Modráčková (seminar tutor)
RNDr. Alois Orlita, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Příhoda, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Chemistry of Conservation - Restoration (programme PřF, B-CH)

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

C5990 Aplikovaná enzymologie

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Petr Skládal, CSc. (lecturer)

Guaranteed by

prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.

Prerequisites

basic overview of biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Applied Biochemistry (programme PřF, B-CH)

Course objectives

An advanced lecture. Enzymes preparation and storage. Enzyme nomenclature. Enzyme structure and kinetics. Enzyme analysis. Use of enzymes in biotechnology. Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques.

Syllabus

• 1. Intial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.

Assessment methods (in Czech)

ústní zkouška nebo kolokvium

Language of instruction

Czech

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C5991 Applied enzymology - seminars
  NOW(C5990)  

• Enrolment Statistics (recent)