C6770 NMR Spectroscopy of Biomolecules

Faculty of Science
Spring 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Radovan Fiala, CSc. (lecturer)
Mgr. Jaromír Toušek, Dr. (lecturer)
prof. Mgr. Lukáš Žídek, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Lukáš Žídek, Ph.D.
Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Lukáš Žídek, Ph.D.
Prerequisites
Familiarity with principles of quantum mechanics will be helpful but is not required.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course will provide introduction to modern NMR techniques which can be applied to extract structural information for small and mid-size biological macromolecules - peptides, proteins, DNA and RNA oligonucleotides. Experimental procedures and computational protocols for determination of three-dimensional structures and dynamics based on NMR data will be discussed.
Syllabus
  • 1. Principles of NMR Spectroscopy I 2. Principles of NMR Spectroscopy II 3. Basic NMR Experiments 4. Protein Structure Determination I (basic strategy, sequential assignment, side-chain assignment) 5. Protein Structure Determination II (nuclear Overhauser efect and internuclear distances, three-bond scalar couplings and torsion angles, other spatial restraints) 6. Protein Structure Determination III (secondary structure determination, intermolecular complexes, large proteins and membrane proteins) 7. Nucleic Acid Structure Determination I 8. Nucleic Acid Structure Determination II 9. Structure Refinement Using Molecular Dynamics 10. Dynamics of Biomolecules I (dynamics of molecules and NMR relaxation, theory of relaxation) 11. Dynamics of Biomolecules II 12. Review
Literature
  • CAVANAGH, John and Wayne J. FAIRBROTHER. Protein NMR Spectroscopy. Principles and Practice. San Diego: Academic Press, 1996, 587 pp. ISBN 0-12-164490-1. info
Assessment methods (in Czech)
Oral examination
Language of instruction
English
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Teacher's information
http://www.ncbr.chemi.muni.cz/~lzidek/C6770.html
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Spring 2004, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2004/C6770