Course Information

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Taught in person.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Petr Lapčík, Ph.D. (assistant)
Mgr. David Novák (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

The aim of the laboratory course is to practically learn the advanced biochemistry methods. The students will perform isolation and separation of biomolecules (nucleic acids, proteins, low molecular compounds), methods for enzyme activity determination, methods for determination of mRNA and protein levels (qPCR, proteomics based on quantitative mass spectrometry), will work with tissue cultures and with modern diagnostics methods, and will process, statistically evaluate and interpret the obtained data.

Learning outcomes

Student will be able to: - Perform native electrophoresis and determine enzyme activity - Isolate proteins from cancer cell lines and determine total protein concentration - Digest proteins using trypsin and purify resulting peptides within a proteomics experiment - Perform database search of proteomics LC-MS/MS data, statistically evaluate and interpret the results - Perform molecular cloning - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform PCR-RFLP analysis - Conduct critical evaluation of obtained data

Syllabus

• Assignment A: Detection of isoenzymes for ascorbate peroxidase by native PAGE and determination of phenylalanin-amoniak lyase activity Assignment B: Comparison of cancer cell line proteotypes using quantitative mass spectrometry Assignment C: Cloning of PCR product into plasmid Assignment D: Identification of an unknown elicitin based on its activity Assignment E: Identification of Armillara sp. in soil samples

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Student have to participate in all parts of the course and submit required protocols.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Taught in person.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Petr Lapčík, Ph.D. (assistant)
Mgr. David Novák (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 15:00–19:50 C15/211

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 15/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

The aim of the laboratory course is to practically learn the advanced biochemistry methods. The students will perform isolation and separation of biomolecules (nucleic acids, proteins, low molecular compounds), methods for enzyme activity determination, methods for determination of mRNA and protein levels (qPCR, proteomics based on quantitative mass spectrometry), will work with tissue cultures and with modern diagnostics methods, and will process, statistically evaluate and interpret the obtained data.

Learning outcomes

Student will be able to: - Perform native electrophoresis and determine enzyme activity - Isolate proteins from cancer cell lines and determine total protein concentration - Digest proteins using trypsin and purify resulting peptides within a proteomics experiment - Perform database search of proteomics LC-MS/MS data, statistically evaluate and interpret the results - Perform molecular cloning - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform PCR-RFLP analysis - Conduct critical evaluation of obtained data

Syllabus

• Assignment A: Detection of isoenzymes for ascorbate peroxidase by native PAGE and determination of phenylalanin-amoniak lyase activity Assignment B: Comparison of cancer cell line proteotypes using quantitative mass spectrometry Assignment C: Cloning of PCR product into plasmid Assignment D: Identification of an unknown elicitin based on its activity Assignment E: Identification of Armillara sp. in soil samples

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Student have to participate in all parts of the course and submit required protocols.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Taught in person.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Petr Lapčík, Ph.D. (assistant)
Mgr. David Novák (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 15:00–19:50 C15/211

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 3/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

The aim of the laboratory course is to practically learn the advanced biochemistry methods. The students will perform isolation and separation of biomolecules (nucleic acids, proteins, low molecular compounds), methods for enzyme activity determination, methods for determination of mRNA and protein levels (qPCR, proteomics based on quantitative mass spectrometry), will work with tissue cultures and with modern diagnostics methods, and will process, statistically evaluate and interpret the obtained data.

Learning outcomes

Student will be able to: - Perform native electrophoresis and determine enzyme activity - Isolate proteins from cancer cell lines and determine total protein concentration - Digest proteins using trypsin and purify resulting peptides within a proteomics experiment - Perform database search of proteomics LC-MS/MS data, statistically evaluate and interpret the results - Perform molecular cloning - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform PCR-RFLP analysis - Conduct critical evaluation of obtained data

Syllabus

• Assignment A: Detection of isoenzymes for ascorbate peroxidase by native PAGE and determination of phenylalanin-amoniak lyase activity Assignment B: Comparison of cancer cell line proteotypes using quantitative mass spectrometry Assignment C: Cloning of PCR product into plasmid Assignment D: Identification of an unknown elicitin based on its activity Assignment E: Identification of Armillara sp. in soil samples

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Student have to participate in all parts of the course and submit required protocols.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Taught in person.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Petr Lapčík, Ph.D. (assistant)
Mgr. David Novák (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 14:00–18:50 C15/211

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

The aim of the laboratory course is to practically learn the advanced biochemistry methods. The students will perform isolation and separation of biomolecules (nucleic acids, proteins, low molecular compounds), methods for enzyme activity determination, methods for determination of mRNA and protein levels (qPCR, proteomics based on quantitative mass spectrometry), will work with tissue cultures and with modern diagnostics methods, and will process, statistically evaluate and interpret the obtained data.

Learning outcomes

Student will be able to: - Perform native electrophoresis and determine enzyme activity - Isolate proteins from cancer cell lines and determine total protein concentration - Digest proteins using trypsin and purify resulting peptides within a proteomics experiment - Perform database search of proteomics LC-MS/MS data, statistically evaluate and interpret the results - Perform molecular cloning - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform PCR-RFLP analysis - Conduct critical evaluation of obtained data

Syllabus

• Assignment A: Detection of isoenzymes for ascorbate peroxidase by native PAGE and determination of phenylalanin-amoniak lyase activity Assignment B: Comparison of cancer cell line proteotypes using quantitative mass spectrometry Assignment C: Cloning of PCR product into plasmid Assignment D: Identification of an unknown elicitin based on its activity Assignment E: Identification of Armillara sp. in soil samples

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Student have to participate in all parts of the course and submit required protocols.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Taught partially online.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Petr Lapčík, Ph.D. (assistant)
Mgr. David Novák (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 14:00–18:50 C15/211

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

The aim of the laboratory course is to practically learn the advanced biochemistry methods. The students will perform isolation and separation of biomolecules (nucleic acids, proteins, low molecular compounds), methods for enzyme activity determination, methods for determination of mRNA and protein levels (qPCR, proteomics based on quantitative mass spectrometry), will work with tissue cultures and with modern diagnostics methods, and will process, statistically evaluate and interpret the obtained data.

Learning outcomes

Student will be able to: - Perform native electrophoresis and determine enzyme activity - Isolate proteins from cancer cell lines and determine total protein concentration - Digest proteins using trypsin and purify resulting peptides within a proteomics experiment - Perform database search of proteomics LC-MS/MS data, statistically evaluate and interpret the results - Perform molecular cloning - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform PCR-RFLP analysis - Conduct critical evaluation of obtained data

Syllabus

• Assignment A: Detection of isoenzymes for ascorbate peroxidase by native PAGE and determination of phenylalanin-amoniak lyase activity Assignment B: Comparison of cancer cell line proteotypes using quantitative mass spectrometry Assignment C: Cloning of PCR product into plasmid Assignment D: Identification of an unknown elicitin based on its activity Assignment E: Identification of Armillara sp. in soil samples

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Student have to participate in all parts of the course and submit required protocols.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. David Novák (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

there are 8 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Learning outcomes

Student will be able to: - Perform PCR-RFLP analysis - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform 2D electrophoresis - Perform native electrophoresis - Expressed recombinant proteins in E. coli and isolate them - Conduct critical evaluation of obtained data

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Learning outcomes

Student will be able to: - Perform PCR-RFLP analysis - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform 2D electrophoresis - Perform native electrophoresis - Expressed recombinant proteins in E. coli and isolate them - Conduct critical evaluation of obtained data

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Recommended Type of Completion: z (credit). Other types of completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Learning outcomes

Student will be able to: - Perform PCR-RFLP analysis - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform 2D electrophoresis - Perform native electrophoresis - Expressed recombinant proteins in E. coli and isolate them - Conduct critical evaluation of obtained data

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Thu 9:00–13:50 C15/211

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its aktivity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.
The course is taught: in blocks.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/5/0. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.

Timetable

Wed 8:00–11:50 C05/108

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/5/0. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.

Timetable

Thu 10:00–13:50 C05/108

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30

fields of study / plans the course is directly associated with

• Biochemistry (programme PřF, M-CH)

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/5/0. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.

Prerequisites

C4220 Biochemistry-lab.course && C6200 Biochemical Methods
C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24

fields of study / plans the course is directly associated with

• Biochemistry (programme PřF, M-CH)

Course objectives

Main objective of laboratory course is to understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

C7195 Advance laboratory course of biochemistry

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

0/5/0. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30

fields of study / plans the course is directly associated with

there are 7 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

C7195 Advance laboratory course of biochemistry

Extent and Intensity

0/5/0. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.

Teacher(s)

doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)

Guaranteed by

prof. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. Mgr. Tomáš Kašparovský, Ph.D.

Prerequisites

C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30

fields of study / plans the course is directly associated with

• Biochemistry (programme PřF, M-CH)

Course objectives

At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.

Syllabus

• Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein

Literature

• Návody ke cvičením - viz Studijní materiály

Teaching methods

Laboratory course

Assessment methods

Credit course

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

• Enrolment Statistics (Autumn 2024, recent)