C8024 Separation methods B
Přírodovědecká fakultajaro 2024
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2025
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2023
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Informace učitele
- videocon lectures will take place in the MS teams environment. if possible, download the environment application. all MU students have access to it via office 365, see "external services" in IS. i recommend learning to work with the application in advance. the use of a browser version restricts your access to some useful functions. the lecture will be recorded in mp4 format and then uploaded in the study materials in IS. MS teams guides: https://is.muni.cz/auth/do/mu/samostudium/pages_en/02-online.html
- Další komentáře
- Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2022
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Informace učitele
- videocon lectures will take place in the MS teams environment. if possible, download the environment application. all MU students have access to it via office 365, see "external services" in IS. i recommend learning to work with the application in advance. the use of a browser version restricts your access to some useful functions. the lecture will be recorded in mp4 format and then uploaded in the study materials in IS. MS teams guides: https://is.muni.cz/auth/do/mu/samostudium/pages_en/02-online.html
- Další komentáře
- Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2021
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2020
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2019
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2018
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objective of the course is to acquaint students with separation methods based on analyte size, separation in force fields and on membranes; at the end part also with other aspects of analytical separations (chiral separation, optimisation, validation).
- Výstupy z učení
- at the end of the course students should be able:
to master principles of macromolecular separation, mass spectrometry, electromigration separation and memebrane separation
to understand the wide scope of their contemporary implementation and utilisation
to optimisation of separation system, to evaluate its efficiency and to understand basic procedures of method validation. - Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject combining study materials and tuition.
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2017
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
Mgr. Pavla Pospíšilová (přednášející) - Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- 1. separation of macromolecules: history, theoretical principles – macromolecule description, molecular mass; modes of macromolecular separation – SEC (size exclusion chromatography), carry-out of SEC – columns,
- 2. detectors (spectrophotometry, viscosimetry, osmometry, light scattering), HDC (hydrodynamic chromatography), FFF (field-flow fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF).
- 3. electromigration methods: history, theoretical principles – separation in outer field, separation description (ion mobility, EOF electroosmotic flow), efficiency and factors, which influence it (heat, diffusion, dispersion); arrangement of electromigration methods – injection, voltage, separation environment (capillary, membrane, gel, chip).
- 4. detectors (spectrophotometric, laser induced fluorescence, conductivity, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytical information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), planar gel electrophoresis (GE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer electrochromatography, capillary electrochromatography;
- 5. affinity and non-aqueous electrophoresis; mass spectrometry (as a separation method): history, theoretical principles – separation in outer field, mass spectrum, basic terms; ionisation (collision, chemical reaction, electrospray), 6. mass analysis (electric sector, magnetic sector, quadrupole, ion trap, time-of-flight analyser, orbital trap, drift cell, ion cyclotron resonance), tandem mass analysis, hybride mass analysers, ion detection (multiplier, induction), vacuum technique;
- 7. membrane separation: history, theoretical principles – non-equilibrium separation, membrane separation principles, carry-out (batch, continual), membranes (selectivity, materials, fouling), separation space – modules: membrane, spiral-wound, tubular, hollow-fibre, rotation, basic modes of membrane separations: microfiltration, ultrafiltration, nanofiltration, reversed osmosis, electrodialysis, pervaporation, membrane distillation, gas permeation, dialysis.
- 8. chiral separation: definition of chirality; optical rotation dispersity; principals of chiral separation – chiral selectors, chiral separation methods including preparative, chiral selectors free, immobilised and derivatization.
- 9. development of chromatographic method, criteria and optimisation of separation system; single and multiple criterial of separation quality evaluation; validation of analytical separation method; why and how, elemental terms –method identity, method precision, calibration, selectivity, robustness
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2016
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
Mgr. Pavla Pospíšilová (přednášející) - Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- written examination; students are required to understand and be familiar with the principles and their applications. examination consists of three basic questions.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2015
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2014
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2013
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Dodavatelské pracoviště: Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2012
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Dodavatelské pracoviště: Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2011
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2010
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Studijní materiály
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2009
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Metody hodnocení
- the lecture is based on ppt presentation and its explication, presentation its-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because there are no available textbooks covering certain parts of the subject.
oral examination; students are required to understand and be familiar with the principles and its applications; enumerations, listing of facts and suchs would not be required. - Vyučovací jazyk
- Angličtina
- Další komentáře
- Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2012 - akreditace
Údaje z období jaro 2012 - akreditace se nezveřejňují
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Dodavatelské pracoviště: Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Předmět je vyučován každoročně.
Výuka probíhá každý týden.
C8024 Separation methods B
Přírodovědecká fakultajaro 2011 - akreditace
- Rozsah
- 2/0/0. 2 kr. (příf plus uk plus > 4). Doporučované ukončení: zk. Jiná možná ukončení: k.
- Vyučující
- doc. Mgr. Jan Havliš, Dr. (přednášející)
- Garance
- doc. Mgr. Jan Havliš, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta - Předpoklady
- C7023 Separation methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited. - Omezení zápisu do předmětu
- Předmět je otevřen studentům libovolného oboru.
- Cíle předmětu
- main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
- Osnova
- separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
- gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
- electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
- Literatura
- Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
- Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
- PLEVA, Michael A. a Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
- AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
- Výukové metody
- the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject.
- Metody hodnocení
- oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
- Vyučovací jazyk
- Angličtina
- Další komentáře
- Předmět je vyučován každoročně.
Výuka probíhá každý týden.
- Statistika zápisu (nejnovější)