C9940 3-Dimensional Transmission Electron Microscopy (3DEM)

Faculty of Science
Spring 2024
Extent and Intensity
2/0/2. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
Ing. Tibor Füzik, Ph.D. (lecturer)
Mgr. Jiří Nováček, Ph.D. (lecturer)
ing. Ondřej Sháněl, Ph.D. (lecturer), Mgr. Jiří Nováček, Ph.D. (deputy)
Guaranteed by
Mgr. Jiří Nováček, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
FB820
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The course will comprise lectures on advanced topics in the field of life-science (cryo-)electron microscopy (cryo-EM). The course will provide practical in-depth insight into advanced sample preparation approaches, provide detailed information about electron miscroscope construction, principles of image formation and data acquisition, and will focus on the advanced methods of cryo-EM and cryo-electron tomography data analysis.
Learning outcomes
Following the successful completion of the course, the students shall be capable: - select optimal strategy to solve different biological problems using electron microscopy - understand the principles of electron microscopy - understand the functionality of the electron microscope - analyze single particle cryo-EM data - analyze cryo-electron tomography data
Syllabus
  • 1. Advanced sample preparation approaches
  • 2. Propagation of electron wave through the microscope
  • 3. Mechanisms of contrast formation in transmission and scanning electron microscopy
  • 5. Image aberrations, image analysis in 2D
  • 6. Scanning transmission electron microscopy, energy dispersive x-ray spectroscopy, zero-loss imaging, EELS
  • 7. electron diffraction tomography, dark-field imaging, FIB/SEM microscopy
  • 8. Practical aspects of single particle analysis
  • 9. Practical aspects of cryo-electron tomography and sub-volume averaging
Teaching methods
Lectures, practicals
Assessment methods
Semester work, written examination
Language of instruction
English
Further Comments
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.
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  • Permalink: https://is.muni.cz/course/sci/spring2024/C9940