F9600 Spektroskopické studium biopolymerů

Faculty of Science
Spring 2012
Extent and Intensity
1/1. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
prof. Dr. Jiří Kozelka, PhD. (lecturer)
prof. RNDr. Tomáš Polívka, Ph.D. (lecturer)
doc. Mgr. Petr Táborský, Ph.D. (lecturer)
doc. RNDr. Oldřich Vrána, CSc. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: prof. Dr. Jiří Kozelka, PhD.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Prerequisites
The course is conceived for graduate students, with a bachelor's degree in physics, chemistry or biology. Basic knowledge of the principles of spectroscopic methods is required. It is recommended to follow the course F6530 (Spectroscopic methods) first.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course explains which structural information about molecules of biopolymers can be acquired using spectroscopic methods (UV-VIS, CD, Luminescence, IR, Raman, NMR, EPR). The succesful student will be able to decide which method to use for the study of a specific biopolymer. Furthermore, he/she will become acquainted with the basic spectroscopic properties of DNA, RNA, and proteins.
Syllabus
  • 1. Interaction of electromagnetic field with electric and magnetic dipole of molecules. Spontaneous and stimulated transitions, Einstein coefficients.. Chirality of molecules, circular dichroism. Application of absorption and CD spectroscopy on the study of biopolymers. 2. Luminescence spectroscopy Jablonski diagram, Franck-Condon principle. Luminescence: fluorescence, phosphorescence and delayed fluorescence. Emission and excitation spectra, time resolved luminescence. Native luminescence, fluorescence labels and probes. 3. Molecular symmetry and vibrational spectra. Selection rules for vibrational transitions, infrared spectroscopy, Raman scattering. 4. NMR Strategies for resonance assignment and for the exploitation of NMR-derived structural data in the construction of 3D-models of biomolecules will be explained using specific examples. Focus will be put on molecular modeling of nucleic acids. 5. EPR The use of EPR data in structural studies on biomolecules will be discussed using selected examples of metalloproteins and ribozymes. 6. Applications The last 4 lectures will be devoted to the solution of specific structural problems. The trainees will decide which method has to be used in which case, and how.
Teaching methods
The course will be tought in blocks of 3-4 lessons.
Assessment methods
Colloquium in the form of a written or an oral test
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2012, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2012/F9600