CC050 FFF-Macromolecule Separation Method

Faculty of Science
Spring 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).
Teacher(s)
doc. RNDr. Bc. Jiří Pazourek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Bc. Jiří Pazourek, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Prerequisites
C7021 Separation Methods A
Separation methods, e.g. C7021
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 28 fields of study the course is directly associated with, display
Course objectives
A new separation method for separation of macromolecules and particles, which does not employs the chemical affinity. The principle was exactly described mathematically by J.C.GIddings.
Syllabus
  • History of Field-flow fractionation (FFF). Physical principles of FFF. Derivation of retention ration for the normal mode of FFF. Other modes ("steric", hyperlayer, focusing). Techniques of FFF: thermal FFF, flow FFF, sedimentation FFF, Gravitational FFF, electric FFF. Application of FFF for physical measurements, analytical use - separation and characterization of macromolecules.
Literature
  • DETERMANN, Helmut. Gelová chromatografie. 1. vyd. Praha: Academia, 1972, 218 s. info
  • KARDOŠ, Emil and Dušan BEREK. Základy kvapalinovej chromatografie. 1. vyd. Bratislava: Alfa, 1979, 285 s. info
  • POPL, Milan and Jaroslav KUBÁT. Základy chromatografie. 2. přeprac. vyd. Praha: SNTL - Nakladatelství technické literatury, 1981, 151 s. info
  • GIDDINGS, John Calvin. Unified Separation Science. Wiley, 1991, 352 pp. ISBN 0-471-52089-6. info
Assessment methods (in Czech)
přednáška - zkouška
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
General note: předmět pro doktorský studijní program Chemie.
Teacher's information
http://www.chemi.muni.cz/~pazourek/student/sepmetody.html
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006.
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