CG010 Proteomics

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
basics in biochemistry, analytical and physical chemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
at the end of the course students should be able to: understand and explain terms related to proteomics; work with information on proteins and their complexes to follow their role in systems biology;
Syllabus
  • Jan Hejátko
  • inroduction into proteomics – definition of proteome (dynamic p.), relations between genomics and proteomics, proteosynthesis and the basic hierarchy of protein structure, protein properties (size and shape, polarity, charge, reactivity))
  • Jan Havliš
  • expression proteomics - the conditions and regulation of gene products expression (under different conditions, also ontogenically) protein identity: protoprotein, protein splicing (intein, extein), post-translational modifications, transport, localisation of proteins (eg. cell membrane proteins, secretome), degradation, relations between genotype and phenotype
  • Jaromír Marek
  • structural proteomics - domains, tertiary structure, protein folding, structure and environment relations, allostery, structure modelling
  • Jan Paleček
  • functional proteomics - the role of proteins (enzymes, signalling molecules, transport molecules, structural molecules), relations between structure and function (domains, protein complexes), relations between localisation and function (interactive maps), relations between the content (and form) of protein and its function, interaction with aprotic molecules
  • Zbyněk Zdráhal
  • bioinformatics and proteins - Mw, pI, primary structure, PTMs, domains, tertiary structure, protein complexes, gene ontology (GO); phylogenetic similarity, the effect of mutations on the structure and function
  • Zbyněk Zdráhal
  • importance and use of proteomics - the contextual analytical proteomics, diagnostic proteomics (biomarkers), single cell proteomics, proteomic aspects of systems biology
Literature
    recommended literature
  • TWYMAN, R.M. Principles of proteomics. BIOS Scientific Publishers, 2008. ISBN 1 85996 273 4. info
  • Proteomics. Edited by Timothy Palzkill. New York: Kluwer Academic Publishers, 2002, viii, 127. ISBN 0-306-46895-6. info
Teaching methods
the lecture is based on ppt presentations and their explication. presentations them-self will be available as study materials (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because there are no available textbooks in czech language covering certain parts of the subject.
Assessment methods
oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.