Information System of Masaryk University 

Course Information

Bi4091c Practise from general microbiology

Faculty of Science
Spring 2008

Extent and Intensity

0/4/0. 4 credit(s). Type of Completion: z (credit).

Teacher(s)

Ing. Martin Krsek, CSc. (seminar tutor)
Mgr. Marcel Kosina (seminar tutor)
PharmDr. RNDr. Jiří Štěpán, Ph.D. (seminar tutor)
Mgr. Andrea Vávrová Pascutti (seminar tutor)

Supervisor

RNDr. Ludmila Tvrzová, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: RNDr. Ludmila Tvrzová, Ph.D.

Timetable

Tue 7:00–10:50 Bpb,02012

Prerequisites (in Czech)

NOW ( Bi4090 General microbiology )

Course Enrollment Limitations

The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrollment status: enrolled: 0/20, only registered: 0/20

Fields of study the course is directly associated with

• General Biology (programme PřF, B-BI, specialization Microbiology)

Course objectives

The aim of the practical course of general microbiology are basic methods used in microbiological laboratory (methods of sterile inoculation and cultivation, slides preparation and microscopic examination of microorganisms, determination of the number of bacteria in different samples).

Syllabus

• 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms, culture collections 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of bacteria by cultivation method. Nephelometric determination of growth of cells 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized microsystems 9. Yeasts, test of vitality 10. Microorganisms in soil. 11. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negativ - staining, capsule - staining. Microscopic examination of endospores. 12. Protocols, test and unknown strain microscopic examination.

Literature

• JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996. 67 pp. ISBN 80-210-1374-5. info

Assessment methods (in Czech)

Podmínkou zápočtu je účast na cvičení, vypracování protokolů, úspěšné zpracování zápočtového preparátu a zápočtového testu.

Follow-Up Courses

• Bi5420 General virology
• Bi6700 Taxonomy of prokaryotes
• Bi6721 Special Methods of Microorganisms Analysis I.
• Bi7330 Cytology and morphology of bacteria

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

Teacher's information

http://www.sci.muni.cz/mikrob/obecna.html
Práce v mikrobiologické laboratoři bezpodmínečně vyžaduje pracovní oděv - studenti si přinesou laboratorní plášť a přezůvky.

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, spring 2012, spring 2012 - acreditation, spring 2013, spring 2014.

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• Enrollment Statistics (Spring 2008, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2008/Bi4091c

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