The course gives the students an opportunity to get acquainted with a number of methods suitable for application in ecotoxicology. It includes both theoretical lectures and practical illustrations of in vivo methods (experiments with laboratory and wild animals) and in vitro methods (experiments using tissue cultures). Besides basic scientific methodology, the students will be given lectures on principles and the use of modern equipment, such as flow cytometer, confocal microscope and other methods, including molecular biology, gas and liquid chromatography, fluorimetry, spectrophotometry and luminometry.
1. In vivo methods Experiments with laboratory animals Basic hematology methods The use of ionising irradiation Tests of toxicity 2. In vitro methods Cell cultures - Laboratory Equipments Basic methodology 3. Detection of biochemical markers Enzyme activities Oxidative stress 4. Selected molecular biology methods Flowcytometry Microscopy 5. Studies of effects of harmful substances and pharmaceuticals Cytotoxicity assays Analysis of cytokinetics 6. Working hypothesis and their verifications 7. Application in ecotoxicology and predictive oncology
Current Protocols in Cytometry, Ed. J. P. Robinson et al., Wiley&Sons Inc., New York 1997
Arachidonate related lipid mediators, Methods in Enzymology, Eds. R. C. Murphy, F. A. Fitzpatrick, Vol. 187, Academic Press, New York, 1990
Techniques in cell cycle analysis (Eds. J. W. Gray, Z. Darzynkiewicz), Humana Press, Clifton, New Jersey, 1987
Cell and tissue culture :Laboratory procedures. Insertion instructions. Edited by A. Doyle - J. B. Griffiths - D. G. Newell. Chichester: John Wiley & Sons, 1993. Volné lis. ISBN 0-471-92852-6. info