Main objective of the lectures is to introduce immune system as the organ sensitive to external factors including toxic chemicals. Students will learn about immunotoxicology - research branch studying effects of stressors (mainly chemical compounds) on immune system and the importance of immune system changes for organism health. Students will be able to describe and discuss immune system, its components, function, communication etc. Students will learn about experimental design in immunotoxicology, and will be able to use, interpret and analyze results of the tiered approach, laboratory methods in immunotoxicology, including discussions of factors affecting immune system. Students will be able to classify toxicants (drugs, pollutants, etc.), and describe ecological immunotoxicology (invertebrates, fish, amphibians).
1. Immunotoxicology. Introduction, history, aims and scopes, human immunotoxicology, ecological immunotoxicology. 2. Immune system. Components of immune system, function, communication, immune system as a target of xenobiotics. Immune system as a part of an organism - interactions, controls, homeostasis, (xeno-)estrogens. 3. Experimental design in immunotoxicology. Exposure, response. "Tier I" and "Tier II" methods. Animal models in immunotoxicology, batteries of immunotoxicology tests. 4. Laboratory methods in immunotoxicology. Assays of immunocompetence, macrophage function assays, host resistance, antitumor immunity, hypersensitivity, autoimmunity. 5. Special immunotoxicology 1 - introduction. Factors affecting immune system (climate, geography, biorhythms, stress, social habits, chemicals, irradiation and other physico-chemical factors). 6. Special immunotoxicology 2 - immunotoxicology of drugs (antibiotics, important pharamceuticals, immunosuppressives a immunomodulators, antitumor therapy). 7. Special immunotoxicology 3 - immunotoxicology of pollutants (metals, pesticides, industrial chemicals - PCBs, PAHs, PCDD(F)s, other organic pollutants, azbestosis, silikosis). 8. Ecological immunotoxicology. Animal immune system and interactions with xenobiotics. Invertebrates, fish, amphibians, birds, mammals. Markers of immune system alterations, ecological consequences. Case studies. 9. Risk assessment. Immunotoxicology as a branch of general toxicology, hazard identification, uncertainities.
Immunotoxicology and immunopharmacology. Edited by Jack H. Dean. 2nd ed. New York: Raven Press, 1994. xxi, 761 s. ISBN 0-7817-0219-4. info
Wagner, V., Wagnerová, M. Ekoimunologie, Avicenum, 1988, Praha
Ferenčík, M. Immunochémia, Alfa, 1989, Bratislava
lectures weekly, e-learning (is.muni.cz), final test and oral examination