Bi7030c Bacterial physiology - practical course

Faculty of Science
autumn 2011
Extent and Intensity
0/5/0. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
Ing. Martin Krsek, CSc. (seminar tutor)
Mgr. Andrea Vávrová Pascutti (seminar tutor)
Supervisor
doc. RNDr. Miroslav Němec, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Miroslav Němec, CSc.
Timetable
Tue 7:00–11:50 Bpb,02012
Course Enrollment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
Fields of study the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand principals of work with bacterial cells and bacteriophages and to understand chosen tasks, to solve various problems they can face in bacterial physiology; to use set of chosen techniques used in physiological laboratories; to work with growth characteristics of bacterial cells and phages, influence of temperature and UV on lysogenic cells and phages; to prepare crude enzymatic preparation and to test its enzymatic activity.
Syllabus
  • Determination of the bacterial proteins and dry-weight. Bacterial growth curve in static culture (using plate counts). One-step growth curve of the polyvalent and virulent staphylopage. Determination of dehydrogenase activity. Preparation of the crude enzymatic preparation (HEP) with phosphatase activity. Determination of phosphatase activity of native cells and HEP. Oxygen consumption (Oxi-Top). Biodegradation. Influence of heavy metals on bacterial growth (Bioscreen).
Literature
  • Schemata pro jednotlivé úlohy poskytne vyučující
  • Horáková, D., Němec, M. (2003) : Laboratorní cvičení z fyziologie bakterií
Teaching methods
Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
Assessment methods
Active participation at practical course, reports, credit.
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
Cvičení nelze individuálně nahrazovat během semestru vzhledem k náročné přípravě materiálu.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, autumn 2012, autumn 2013.
  • Enrollment Statistics (autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2011/Bi7030c

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