Bi7941 Molecular biology - practice

Faculty of Science
Autumn 2015
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Marian Varga, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi7941/01_1: Thu 8:00–13:50 D36/216, M. Varga
Bi7941/02_1: Thu 14:00–19:50 D36/216, M. Varga
Bi7941/02_2: Thu 14:00–19:50 D36/216, M. Varga
Prerequisites
Bi4020 Molecular biology || NOW( Bi4020 Molecular biology )
Basic knowledge of general genetics, microbiology, anorganic chemistry, organic chemistry and biochemistry.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This practical course is conceived as an introduction into methods of molecular biology. At the end of the course students should be able to analyse the genetic material including chromosomal DNA and solve cloning experiments with plasmids originating from the bacteria.
Syllabus
  • 1. Preparation of solutions and stock enzymes used in molecular biology.
  • 2. Isolation of plasmid DNA (pUC family, pBluescript). Determination of DNA concentration and purity.
  • 3. Restriction analysis of nucleic acids, agarose gel electrophoresis.
  • 4. Restriction mapping, isolation of DNA from agarose gels.
  • 5. DNA cloning in basic types of vectors, DNA transfer to prokaryotic cells, transformation, selection of clones.
  • 6. Centrifugation techniques, preparation of saccharose density gradients.
  • 7. Isolation of bacterial genomic DNA for DNA fingerprinting.
  • 8. Polymerase chain reaction and their modifications (PCR-RFLP, AP-PCR).
Literature
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The practical course is taught in seven 6-hour blocks in a molecular biology laboratory. Students perform the methods according to examined protocols.
Assessment methods
The course is completed after work out an elaborate from the performed tasks and pass a written test which contains 20 practically oriented questions.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2015, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2015/Bi7941