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Bi8858 Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology

Faculty of Science
autumn 2012

The course is not taught in autumn 2012

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. Radka Chaloupková, Ph.D. (lecturer)

Supervisor

prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Zbyněk Prokop, Ph.D.
Dodavatelské pracoviště: Department of Experimental Biology - Biology Section - Faculty of Science

Prerequisites

! C8858 Biocatalysis
Basic course of biochemistry and microbiology recommended.

Course Enrollment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

Fields of study the course is directly associated with

there are 27 fields of study the course is directly associated with, display

Course objectives

Enzyme technology – a subfield of biotechnology – offers modern solutions for the development of new products, processes and services to meet various human needs. The goal of this inovative approach utilising enzymes as biocatalysts is not only to design competetive processes but also meet criteria of sustainability (reduction of raw material, energy and water consumption, reduction of waste production and overall environmental impact together with increasing usage of renewable materials such as biomass). The aim of the course is to provide insight into the fundamentals of enzyme technology and applied biocatalysis. The course covers all important steps of multidisciplinary process of enzyme technology development overlapping areas of cell and molecular biology, biochemistry and process engineering. The examples of succesfull “green” enzyme technology aplications are presented in the course.

At the end of this course, students should be able to: be familiar with the interdisciplinary approach required to utilize the catalytic potential of natural biomolecules, enzymes, or whole cells for the production of useful compounds understand the concept of sustainability and modern, environmentaly acceptable trends in industrial chemistry understand the basic principles of enzymatic reactions and when these catalysts are prefered have overview of reaction types, conditions and method for monitoring of biocatalytic proceses have knowledge how to obtain and optimise/improve enzyme catalysts select optimal biocatalyst (enzyme) for desired reaction design the basic biocatalytic process

Syllabus

1. Opening Lecture
2. Introduction to Enzyme Technology
3. Basics of Enzymes as Biocatalysts
4. Kinetics of Enzyme Catalysed Reaction
5. Principles and Application of Enzyme Selectivity
6. Enzymes in Organic Synthesis I.
7. Enzymes in Organic Synthesis II.
8. Enzyme Production and Purification
9. Engineering of Biocatalysts
10. Immobilization of Enzymes and Cells
11. Reaction Engineering and Process Technology
12. Biotechnology Informatics

Literature

recommended literature

BUCHHOLZ, Klaus, Volker KASCHE and Uwe Theo BORNSCHEUER. Biocatalysts and enzyme technology. Weinheim: Wiley-VCH, 2005. xvii, 448. ISBN 3-527-30497-5. info
FABER, Kurt. Biotransformations in organic chemistry :a textbook. 5th rev. and corr. ed. Berlin: Springer-Verlag, 2004. xi, 454 s. ISBN 3-540-20097-5. info

Teaching methods

lectures with powerpoint presentation and pdf materials for printing at http://loschmidt.chemi.muni.cz/peg/, short training written tests at the beginning of each lecture and short exercises during lecture

Assessment methods