Bi8858 Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology
Faculty of Science
The course is not taught in autumn 2012
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- doc. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. Radka Chaloupková, Ph.D. (lecturer)
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Zbyněk Prokop, Ph.D.
Dodavatelské pracoviště: Department of Experimental Biology - Biology Section - Faculty of Science
- ! C8858 Biocatalysis
Basic course of biochemistry and microbiology recommended.
- Course Enrollment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- Fields of study the course is directly associated with
- there are 27 fields of study the course is directly associated with, display
- Course objectives
- Enzyme technology – a subfield of biotechnology – offers modern solutions for the development of new products, processes and services to meet various human needs. The goal of this inovative approach utilising enzymes as biocatalysts is not only to design competetive processes but also meet criteria of sustainability (reduction of raw material, energy and water consumption, reduction of waste production and overall environmental impact together with increasing usage of renewable materials such as biomass).
The aim of the course is to provide insight into the fundamentals of enzyme technology and applied biocatalysis. The course covers all important steps of multidisciplinary process of enzyme technology development overlapping areas of cell and molecular biology, biochemistry and process engineering. The examples of succesfull “green” enzyme technology aplications are presented in the course.
At the end of this course, students should be able to:
be familiar with the interdisciplinary approach required to utilize the catalytic potential of natural biomolecules, enzymes, or whole cells for the production of useful compounds
understand the concept of sustainability and modern, environmentaly acceptable trends in industrial chemistry
understand the basic principles of enzymatic reactions and when these catalysts are prefered
have overview of reaction types, conditions and method for monitoring of biocatalytic proceses
have knowledge how to obtain and optimise/improve enzyme catalysts
select optimal biocatalyst (enzyme) for desired reaction
design the basic biocatalytic process
- 1. Opening Lecture
- 2. Introduction to Enzyme Technology
- 3. Basics of Enzymes as Biocatalysts
- 4. Kinetics of Enzyme Catalysed Reaction
- 5. Principles and Application of Enzyme Selectivity
- 6. Enzymes in Organic Synthesis I.
- 7. Enzymes in Organic Synthesis II.
- 8. Enzyme Production and Purification
- 9. Engineering of Biocatalysts
- 10. Immobilization of Enzymes and Cells
- 11. Reaction Engineering and Process Technology
- 12. Biotechnology Informatics
- BUCHHOLZ, Klaus, Volker KASCHE and Uwe Theo BORNSCHEUER. Biocatalysts and enzyme technology. Weinheim: Wiley-VCH, 2005. xvii, 448. ISBN 3-527-30497-5. info
- FABER, Kurt. Biotransformations in organic chemistry :a textbook. 5th rev. and corr. ed. Berlin: Springer-Verlag, 2004. xi, 454 s. ISBN 3-540-20097-5. info
- Teaching methods
- lectures with powerpoint presentation and pdf materials for printing at http://loschmidt.chemi.muni.cz/peg/, short training written tests at the beginning of each lecture and short exercises during lecture
- Assessment methods
- written test in English, 45 questions, maximum 45 points (successfulness/classification: >40/A, 30-39/B, 22-29/C, 16-21/D, 11-15/E, <11/F)
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Teacher's information
The course is also listed under the following terms
Autumn 2010 - only for the accreditation
Autumn 2011 - acreditation
- Enrollment Statistics (autumn 2012, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2012/Bi8858