Information System of Masaryk University 

Course Information

C8160 Enzymology

Faculty of Science
Spring 2010

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Alternate Types of Completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Supervisor

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry - Chemistry Section - Faculty of Science

Timetable

Tue 8:00–9:50 409-stara KAM1

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry, organic chemistry and physical chemistry are recommended.

Course Enrollment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

Fields of study the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should have basic idea about the properties of enzymes and the relationship between their structure and mechanisms of catalytic action. He should know the most important representatives of individual classes, their use in practice and basic methods for the determination of enzyme activity. It is assumed that they will be able to process data from kinetic studies and interpret the results in relation to the reaction mechanism.

Syllabus

• 1) First sight into the world of enzymes: structure, specifity, nomenclature. 2) Enzyme activity and their measurement. 3) Isolation of enzymes from the natural sources and expression systems. 4) Enzyme catalysis from the point of view of organic chemistry. 5) Enzyme catalysis from the point of view of physical chemistry. 6) Basic theoretical concepts in enzyme kinetics. 7) Effects of environmental factors (pH, temperature) on enzymes. 8) Reversible and irreversible inhibition of enzymes. 9) Kinetics of multisubstrate enzyme reactions. 10) Cooperativity in enzyme function. 11) Immobilized enzyme systems. 12) Enzymes as tools in bioanalysis. 13) Use of enzymes in technology and medicine. Enzyme engineering.

Literature

recommended literature
• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994. 152 s. ISBN 80-210-1039-8. info
• KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987. 121 pp. info
• KOTYK, Arnošt and Jaroslav HORÁK. Enzymová kinetika. 1. vyd. Praha: Academia, 1977. 268 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8170 Enzymology - seminar
  NOW(C8160)  

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, spring 2012, spring 2012 - acreditation, spring 2013, spring 2014.

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• Enrollment Statistics (Spring 2010, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2010/C8160

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