The aim of the course is to offer basic information about structure determination of biomacromolecules (especially proteins and nucleic acids). The course is designed as a general overview offered to students who do not plan to enter this research area, but it may serve as an introduction to advanced courses of structure analysis. An introductory overview of basic structural motifs will be followed by discussion of methods of determination of 3D structure of macromolecules. The course will be focused on two fundamental techniques - X-ray crystallography and NMR spectroscopy. Methods of molecular mechanics and dynamics used in structure calculations based on experimental data will be described. Techniques of molecular biology utilized in protein structure determination will be discussed. Use of structure databases will be mentioned. Lectures will be presented by experts active in the discussed research areas.
1-3. Structure of macromolecules, basic structural motifs of proteins and nucleic acids, structure of saccharides and membranes.
4-5. Computational methods, molecular mechanics and dynamics, simulated annealing.
6. Nucleic acid sequencing, techniques of gene engineering, expression of recombinant proteins.
7. General characterization of proteins, methods of optical and mass spectroscopy, protein sequencing.
8-9. X-ray crystallography. Crystal preparation, diffraction experiment, methods of solving phase problem, electron density maps, structure model building.
10-11. Nuclear magnetic resonance. Isotope labeling, NMR experiment, spectral frequency assignment, determination of geometry (NOE, coupling constants), protein dynamics.
12. Databases of structures, bioinformatics, computer prediction and modeling.
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.