VLLM0421c Medical Microbiology I - practice

Faculty of Medicine
Spring 2012
Extent and Intensity
0/3/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
RNDr. Radek Horváth, Ph.D. (seminar tutor)
Mgr. Martina Mahelová (seminar tutor)
Mgr. Barbora Mališová, Ph.D. (seminar tutor)
Mgr. Tereza Peroutková (seminar tutor)
doc. MUDr. Filip Růžička, Ph.D. (seminar tutor)
MUDr. Alena Siváková (seminar tutor)
Mgr. Petra Šišková (seminar tutor)
doc. MUDr. Vladana Woznicová, Ph.D. (seminar tutor)
Ing. Magdalena Flasarová (assistant)
Supervisor
doc. MUDr. Filip Růžička, Ph.D.
Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Dodavatelské pracoviště: Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Timetable of Seminar Groups
VLLM0421c/01_02 Mon 15:45–18:15 N02112, A. Siváková
VLLM0421c/03_04 Tue 13:00–15:30 N02112, M. Dvořáková Heroldová
VLLM0421c/05_06 Wed 10:15–12:45 N02112, O. Zahradníček
VLLM0421c/07_08 Mon 7:30–10:00 N02112, O. Zahradníček
VLLM0421c/09_10 Thu 13:45–16:15 N02112, L. Černohorská
VLLM0421c/11_12 Mon 13:00–15:30 N02112, O. Zahradníček
VLLM0421c/13_16 Tue 16:00–18:30 N02112, M. Dvořáková Heroldová
VLLM0421c/14_15 Wed 13:00–15:30 N02112, B. Mališová
VLLM0421c/17_18 Fri 7:30–10:00 N02112, T. Peroutková
VLLM0421c/19_20 Wed 7:30–10:00 N02112, M. Mahelová
VLLM0421c/30_31 O. Zahradníček
VLLM0421c/32_33 O. Zahradníček
VLLM0421c/41 Fri 10:30–13:00 N02112, P. Šišková
Prerequisites
VSLC011p Med.Chem.- lecture && VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. )
Students are supposed to have considerable knowledge of anathomy, histology, biology, biochemistry and physiology.
Course Enrollment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
Fields of study the course is directly associated with
Course objectives
At the end of this course, students should be able to understand principles of following diagnostic methods in medical microbiology:
microscopy (wet mount, Gram staining, other staining methods)
culture (culture media, theire use, differences between them)
identification of bacteria and yeasts (various methods of biochemical identification, other identification methods)
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
nucleic acid detection in microbiology (only basically)
methods detecting antigens or antibodies, theire use for antibody detection/antigen detection in specimen/antigen analysis of a strain; titers, titer dynamics, detection of IgM/IgA/IgG; precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will understand the basic of medical virology
Syllabus
  • J01 Safety in laboratory, technique of work with loop, wet mount, simple staining. Demostration of pictures of various organisms in simple staining.
  • J02 Gram staining, demonstration of more staining methods. Demostration of pictures of various organisms in Gram staining.
  • J03 Bacterial culture
  • J04 Fysiology of bacteria, idenctification according to biochemical activity and virulence factor determination
  • J05 Microbes and outer influences, decontamination methods
  • J06 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J07 Introduction to serology, agglutination and precipitation, dilution and following titre dynamics
  • J08 Complementfixing test
  • J09 Neutralisatioin (ASO, HIT, VNT)
  • J10 Reactions with labelled components (IMF, RIA, ELISA) including immunoblotting
  • J12 Medical virology I (influenza, respiratory viruses, tick-borne encefalitis)
  • J13 Medical virology II (hepatitis and HIV)
  • J14 Review
Literature
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1. vyd. Brno: Neptun, 2001. 247 pp. ISBN 80-902896-2-2. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1. vyd. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003. 495 pp. ISBN 80-902896-6-5. info
  • VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody (Medical Microbiology: Laboratory Procedures). 1. vyd. Brno: Neptun, 2010. 495 pp. ISBN 978-80-86850-04-7. info
  • GREENWOOD, David. Lékařská mikrobiologie : přehled infekčních onemocnění : patogeneze, imunita, laboratorní diagnostika a epidemiologie. 1. vyd. Praha: Grada Publishing, 1999. 686 s. ISBN 80-7169-365-0. info
  • BEDNÁŘ, Marek. Lékařská mikrobiologie : bakteriologie, virologie, parazitologie. 1. vyd. Praha: Marvil, 1996. 558 s. info
  • RULÍK, Martin, Veronika HOLÁ, Filip RŮŽIČKA, Miroslav VOTAVA, Dana BAUDIŠOVÁ, Jiří GALLO, Simona KAPRÁLOVÁ, Kateřina KOHUŠOVÁ, Dagmar KOUKALOVÁ, Václav KŮDELA, Jiří MIKEŠ, Radko NOVOTNÝ, Martina SIGLOVÁ, Věra TORŠOVÁ and Jiří ZIMÁK. Mikrobiální biofilmy (Microbial Biofilms). 1. vydání. Olomouc: Univerzita Palackého v Olomouci, 2011. 448 pp. Monografie. ISBN 978-80-244-2747-8. info
  • Medical microbiology : A guide to microbial infections: Pathogenesis, immunity, laboratory diagnosis and control. Edited by David Greenwood - Richard C. B. Slack - John F. Peutherer - Barer M. 17th ed. Edinburgh: Churchill Livingstone, 2007. 738 s. ISBN 978-0-443-10209-7. info
  • MURRAY, Patrick R., Ken S. ROSENTHAL and George S., et al. KOBAYASHI. Medical microbiology. 6th ed. St. Louis: Mosby, 2008. 960 pp. ISBN 0-323-05470-6. info
  • GOERING, R., ET AL. Mims’ Medical Microbiology, 4th. ed. Edited by Cedric A. Mims. : Mosby, 2007. info
  • Pro české studium jsou základní tituly 1, 2 a protokoly z IS MU, ostatní jsou doplňkové. For English study, title No. 7 is essential, plus protocoles on IS MU. Titles No. 8 and 9 are supplementary.
Teaching methods
practical training in lab
self-study with use of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with following notes:
- two justified absences or absences approved by teacher are allowed; students are obliged to show that they completed the knowledge (in laboratory reports and in their heads)
- more absences than two require a substitution
- not justified absences are not allowed (EXTRA PAYMENT IS REQUIRED FOR EVENTUAL SUBSTITUTION!!!)
(2) complete laboratory report (signature of teacher is not necessary)
(3) succesfully written credit test, eventually more tests; date of all tests will be declared in time.
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.medmicro.info
E-mailový a telefonický kontakt – E-mail and phone contact:

Doc. MUDr. Filip Růžička, PhD: fruzic@fnusa.cz, TEL 543 183 090 (přednosta ústavu/head)

prof. MUDr. Miroslav Votava, CSc.: miroslav.votava@fnusa.cz, TEL 543 183 117

as. MUDr. Ondřej Zahradníček: zahradnicek@fnusa.cz, TEL 543 183 113

odb. as. MUDr. Lenka Černohorská, PhD.: lenka.cernohorska@fnusa.cz, TEL 543 183 093

odb. as. Mgr. Monika Dvořáková Heroldová, PhD: monika.heroldova@fnusa.cz

as. Mgr. Barbora Žaloudiková: barzal@cktch.cz

as. Mgr. Martina Mahelová: 150878@mail.muni.cz

as. Mgr. Petra Šišková: 151271@mail.muni.cz

as. MUDr. Alena Siváková: alena.sivakova@fnusa.cz

as. Mgr. Tereza Peroutková: peroutkova.tereza@fnusa.cz

Další informace jsou průběžně zveřejňovány na nástěnce v~praktikárně ústavu. More information in Practical Hall.

The course is also listed under the following terms Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2013, Spring 2014.
  • Enrollment Statistics (Spring 2012, recent)
  • Permalink: https://is.muni.cz/course/med/spring2012/VLLM0421c

Other references: 

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