Biopesticides are very actual and commercial matter in save area products against insect pest in the whole world. Among insecticides include biological control agent's as are e.g. entomopathogenic bacteria (Bacillus thuringiensis), viruses (polyhedroviruses, baculoviruses), fungi (Bauwessia spp., Metarhysium spp.), entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) and protozoans (Microsporidia, Nosema locustae). Biological insecticides are rare some by features that the no burden environment, in comparison with chemical preparations till this time plentifully used, because it occur in environment naturally. Among benefits pair with their using belongs to: they are thrifty to environment (don't leave residue like chemical insecticides), be safe to non targed organisms and to humans, their application is simple, price is comparable with chemical preparations, they are in environment stable and don't cause resistance of insect. Big importance obtain rare mechanism effect, that is for any caregory of bioinsecticides different. Entomopathogenic bacteria and viruses produce toxins after penetration into host, that the induce septicaemia. Entomopathogenic fungi produce spats that the conidias and intergrow body host, whereby kills him and entomopathogenic nematodes together with symbiotic bacteria (Xenorhabdus, Photorhabdus) gesticulate guts host and multiply there. Meaning of biobiocides is enormous, but their exploitation practically needs yet some improvement. Important is also gain confidence consumers and growers, to more and more happened to restraint using poisonous chemical preparations. On theoretic part tie together experimental part, in which I auditioned methods of isolation entomopathogenic nematodes from accidentally sample soil.