Curriculum vitae

Person Identification

• Prof. RNDr Viktor Brabec, DrSc., born December 30, 1944, in Prostějov, Czechoslovakia, married, 2 childern
  

Workplace

• Faculty of Science
  Kotlářská 2
  CZ-61137 Brno
  Czech Republic
  
  
• Institute of Biophysics
  Academy of Sciences of the Czech Republic
  Královopolská 135
  CZ-61265 Brno
  Czech Republic

Employment Position

• Professor

Education and Academic Qualifications

• 2002: Professor of Biophysics, Masaryk University Brno;
• 1997: Associate Professor of Biophysics, Masaryk University Brno, habilitation work "Biophysical analysis of reactions of DNA with antitumor complexes of platinum group";
• 1986: DrSc. (D.Sc.), thesis "Interactions of biomacromolecules with electrically charged surfaces and drugs, Institute of Biophysics, Czechoslovak Academy of Sciences, Brno;
• 1973: CSc (PhD) in Biophysics, Czechoslovak Academy of Sciences, Prague, thesis "Interactions of nucleic acids with electrically charged surfaces";
• 1972: RNDr in Biophysics (with honors), Faculty of Science, University of Brno.

Employment Summary

• 2002 - : Professor of Biophysics, Faculty of Science, Brno;
• 1998 - 2002: Associate Professor of Biophysics, Faculty of Science, Brno;
• 1986 - : Head, Department/Laboratory, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno;
• 1995 - 2000: International Research Scholar of the Howard Hughes Medical Institute, USA;
• 1991 - 1993: Directeur de Recherche, Centre de Biophysique Moleculaire, CNRS, Orleans, France;
• 1984: Visiting Professor, Yokohama National University, Japan;
• 1973 - 1986: Scientist at various positions, Institute of Biophysics, Czechoslovak Academy of Science, Brno.

Pedagogical Activities

• Molecular biophysics; molecular biophysics of mutagens, cancerogens and cytostatics; supervisor of PhD students in biophysics.

Scientific and Research Activities

• DNA interactions of antitumor metal-based drugs. Electrochemistry and interfacial properties of biomacromolecules. Methods of molecular biophysics and biology in nucleic acids research.

Academical Stays

• 1976 - 1977: Research Associate, Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, USA;
• 1984, 1987: Visiting Professor, Department of Chemistry, Yokohama National University, Yokohama, USA;
• 1991 - 1993: Directeur de Recherche, Centre de Biophysique Moleculaire, CNRS, Orleans, France;
• 1995: Visiting Scientist, Department of Pharmaceutical Chemistry, University of Bari, Bari, Italy.

Projects

• Affection of DNA conformation by antitumor metal complexes. Relations to the development of new cytostatics; principal investigator: Assoc. Prof. RNDr. Viktor Brabec, DrSc; agency: Grant Agency of the Czech Republic; period: 01/01/99 - 31/12/01.
• The effect of geometric isomerization of antitumor dinuclear platinum complexes on binding and conformational alterations in DNA; principal investigator: Assoc. Prof. RNDr. Viktor Brabec, DrSc; agency: Grant Agency of the Academy of Sciences of the Czech Republic; period: 01/01/97 - 31/12/00.
• DNA interactions of platinum-group metals: relations to their antitumor activity; principal investigator: Assoc. Prof. RNDr. Viktor Brabec, DrSc; agency: Howard Hughes Medical Institute (USA); period: 16/08/95 - 15/08/00.
• Mechanistic studies on new platinum agents; principal investigator: Assoc. Prof. RNDr. Viktor Brabec, DrSc; agency: National Institutes of Health, Bethesda, USA; period: 01/07/99 - 30/06/02.
• DNA interactions of polynuclear platinum; principal investigator: Assoc. Prof. RNDr. Viktor Brabec, DrSc; agency: National Science Foundation (USA); period: 01/01/98 - 31/12/00.
• DNA interactions of platinum anticancer drugs. Relation to the development of new cytostatics; principal investigator: Assoc. Prof. RNDr. Viktor Brabec, DrSc; agency: The Wellcome Trust (UK); period: 01/11/00 - 31/10/03.

Appreciation of Science Community

• 1993 - 1997: co-chairman of the division of the International Society of Electrochemistry;
• 1994 - : member of the Management Committee of the EU Action COST D1, D8, D20 and D21;
• 1997 - : chairman of the Scientific Council of the Institute of Biophysics, Brno;
• 1998 - : member of the assembly of the Academy of Sciences of the Czech Republic.

Selected Publications

• HOFR, Ctirad and Viktor BRABEC. Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, vol. 276, No 276, p. 9655-9661. ISSN 0021-9258. info
• KAŠPÁRKOVÁ, Jana, Šárka POSPÍŠILOVÁ and Viktor BRABEC. Differential recognition of DNA modified by antitumor cisplatin and its clinically ineffective trans isomer by tumor suppressor protein p53. In Journal of Biological Chemistry. 2001, p. 16064-16069. info
• KAŠPÁRKOVÁ, Jana, Šárka POSPÍŠILOVÁ and Viktor BRABEC. Different recognition of DNA modified by antitumor cisplatin and its clinically ineffective trans isomer by tumor suppressor protein p53. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol., 2001, vol. 276, No 19, p. 16064-16069. ISSN 0021-9258. info
• HOFR, Ctirad, Nicholas FARRELL and Viktor BRABEC. Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex. Nucleic Acids Research. Oxford, UK: Oxford Press, 2001, vol. 29, No 10, p. 2034-2040. ISSN 0305-1048. info
• MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO and Viktor BRABEC. Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media. Journal of Biological Inorganic Chemistry. germany: Springer-Verlag, 2001, vol. 6, No 1, p. 435-445. ISSN 0949-368. info
• ZEHNULOVÁ, Jana, Jana KAŠPÁRKOVÁ, Nicholas FARRELL and Viktor BRABEC. Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol., 2001, vol. 276, No 25, p. 22191-22199. ISSN 0021-9258. info
• MISSURA, Miriam, Tonko BUTERIN, Robert HINDGES, Ulrich HUBSCHER, Jana KASPARKOVA, Viktor BRABEC and Hanspeter NAEGELI. Double-check probing of DNA bending and unwinding by XPA-RPA: an architectiral function in DNA repair. EMBO Journal. Oxford, UK: Oxford University Press, 2001, vol. 20, No 13, p. 3554-3564. ISSN 1460-2075. info
• KAŠPÁRKOVÁ, Jana, Nicholas FARRELL and Viktor BRABEC. Sequence specificity, conformation, and recognition by HMG1 protein of major DNA interstrand cross-links of antitumor dinuclear platinum complexes. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2000, vol. 275, No 21, p. 15789-15799. ISSN 0021-9258. info
• MALINA, Jaroslav, Ctirad HOFR, Lucciana MARESCA, Giovanni NATILE and Viktor BRABEC. DNA interactions of antitumor cisplatin analogs containing enantiomeric amine ligands. Biophysical Journal. New York, USA: Rockefeller University, 2000, vol. 2000, No 78, p. 2008-2021. ISSN 0006-3465. info
• BRABEC, Viktor, Kamila NEPLECHOVÁ, Jana KAŠPÁRKOVÁ and Nicholas FARRELL. Steric control of DNA interstrand cross-link sites of trans platinum complexes: specificity can be dictated by planar nonleaving groups. Journal of Biological Inorganic Chemistry. Germany: Springer-Verlag, 2000, vol. 2000, No 5, p. 364-368. ISSN 0949-8257. info
• BRABEC, Viktor. DNA sensor for the determination of antitumor platinum compounds. Electrochimica Acta. UK: Pergamon, 2000, vol. 2000, No 45, p. 2929-2932. ISSN 0013-4686. info
• BRABEC, Viktor. Chemistry and structural biology of 1,2-interstrand adducts of cisplatin. In Platinum Based Drugs in Cancer Therapy. Totowa, New Jersey, USA: Humana Press, Inc, 2000, p. 37-61. ISBN 0-89603-599-9. info
• KOSTRHUNOVÁ, Hana and Viktor BRABEC. Conformational analysis of site-specific DNA cross-links of cisplatin-distamycin conjugates. Biochemistry. Washington, USA: American Chemical Society, 2000, vol. 2000, No 39, p. 12639-12649. ISSN 0006-2960. info
• NOVÁKOVÁ, Olga, Ctirad HOFR and Viktor BRABEC. Modification of natural, double-helical DNA by antitumor cis- and trans-[Cl2(Me2SO4)4Ru] in cell-free media. Biochemical Pharmacology. Oxford, UK: Elsevier, 2000, vol. 60, No 12, p. 1761-1771. ISSN 0006-2952. info
• BRABEC, Viktor, Jana KAŠPÁRKOVÁ, Oldřich VRÁNA, Olga NOVÁKOVÁ, John W. COX, Yun QU and Nicholas FARRELL. DNA modifications by a novel bifunctional trinuclear platinum Phase I anticancer agent. Biochemistry. Washington, USA: American Chemical Society, 1999, No 38, p. 6781-6790. ISSN 0006-2960. info
• LOSKOTOVÁ, Hana and Viktor BRABEC. DNA interactions of cisplatin tethered to the DNA minor groove binder distamycin. European Journal of Biochemistry. New Yor, USA: Springer, 1999, No 266, p. 392-402. ISSN 0014-2956. info
• KAŠPÁRKOVÁ, Jana, Olga NOVÁKOVÁ, Oldřich VRÁNA, Nicholas FARRELL and Viktor BRABEC. Effect of geometric isomerism in dinuclear platinum antitumor complexes on DNA interstrand cross-linking. Biochemistry. Washington, USA: American Chemical Society, 1999, No 38, p. 10997-11010. ISSN 0006-2960. info
• NEPLECHOVÁ, Kamila, Jana KAŠPÁRKOVÁ, Oldřich VRÁNA, Olga NOVÁKOVÁ, Abraha HABTEMARIAM, Beth WATCHMAN, Peter SADLER and Viktor BRABEC. DNA interactions of new antitumor aminophosphine platinum(II) complexes. Molecular Pharmacology. Baltimore, USA: Williams and Wilkins, 1999, No 56, p. 20-30. ISSN 0026-895X. info
• MCGREGOR, Tracey, Zdeňka BALCAROVÁ, Yun QU, M.C. TRAN, Renata ŽALUDOVÁ, Viktor BRABEC and Nicholas FARRELL. Sequence-dependent conformational changes in DNA induced by polynuclear platinum complexes. Journal of Inorganic Biochemistry. New York, USA: Elsevier, 1999, No 77, p. 43-46. ISSN 0162-0134. info
• BALCAROVÁ, Zdeňka, Jana KAŠPÁRKOVÁ, Alena ŽÁKOVSKÁ, Olga NOVÁKOVÁ, Maria F. SIVO, Giovanni NATILE and Viktor BRABEC. DNA interactions of a novel platinum drug, cis-[PtCl(NH3)2(N7-acyclovir)]+. Molecular Pharmacology. Baltimore, USA: Williams and Wilkins, 1998, No 53, p. 846-855. ISSN 0026-895X. info
• ŽÁKOVSKÁ, Alena, Olga NOVÁKOVÁ, Zdeňka BALCAROVÁ, Uli BIERBACH, Nicholas FARRELL and Viktor BRABEC. DNA interactions of antitumor trans-[PtCl2(NH3)(quinoline)]. European Journal of Biochemistry. New York, USA: Springer, 1998, No 254, p. 547-557. ISSN 0014-2956. info
• BRABEC, Viktor, Vladimír KLEINWÄCHTER and Vladimír VETTERL. Structure, chemical reactivity and electromagnetic properties of nucleic acids. In Bioelectrochemistry: Principles and Practice. Basel, Switzerland: Birkhäuser Verlag, 1997, p. 1-104. 5. ISBN 3-7643-5296-5. info
• QU, Yun, Nicholas FARRELL, Jana KAŠPÁRKOVÁ and Viktor BRABEC. DNA binding of properties of trinuclear platinum complex. Journal of Inorganic Biochemistry. New York: Elsevier, 1997, No 67, p. 174. ISSN 0162-0134. info
• TANIGUCHI, Isao, Yun MIE, Katsuhiko NISHIYAMA, Viktor BRABEC, Olga NOVÁKOVÁ, S. NEYA and N. FUNASAKI. Electrochemistry of monoazahemin reconstituted myoglobin at an indium oxide electrode. Journal of Electroanalytical Chemistry. Lausanne, Switzerland: Elsevier, 1997, No 420, p. 5-9. ISSN 0022-0728. info
• ŽALUDOVÁ, Renata, Giovanni NATILE and Viktor BRABEC. The effect of antitumor trans-[PtCl2(E-iminoether)2] on B->Z transition in DNA. Anti-Cancer Drug Design. Oxford, UK: Oxford Press, 1997, No 12, p. 295-309. ISSN 0266-9536. info
• ŽALUDOVÁ, Renata, Alena ŽÁKOVSKÁ, Jana KAŠPÁRKOVÁ, Zdeňka BALCAROVÁ, Vladimír KLEINWÄCHTER, Oldřich VRÁNA, Nicholas FARRELL and Viktor BRABEC. DNA interactions of bifunctional dinuclear platinum(II) antitumor agents. European Journal of Biochemistry. New York, USA: Springer, 1997, No 246, p. 508-517. ISSN 0014-2956. info
• ŽALUDOVÁ, Renata, Alena ŽÁKOVSKÁ, Jana KAŠPÁRKOVÁ, Zdeňka BALCAROVÁ, Oldřich VRÁNA, Mauro COLUCCIA, Giovanni NATILE and Viktor BRABEC. DNA modifications by antitumor trans-[PtCl2(E-iminoether)2]. Molecular Pharmacology. Baltimore, USA: Williams and Wilkins, 1997, No 52, p. 354-361. ISSN 0026-895X. info
• BRABEC, Viktor, Vladimír VETTERL and Oldřich VRÁNA. Electroanalysis of biomacromolecules. In Bioelectrochemistry: Principles and Practice. Basel, Switzerland: Birkhäuser Verlag, 1996, p. 287-359. Bioelectrochemistry:Principles and Practice, Vol.3. ISBN 3-7643-5084-9. info
• BRABEC, Viktor, Oldřich VRÁNA, Olga NOVÁKOVÁ, Vladimír KLEINWÄCHTER, Francesco INTINI, Mauro COLUCCIA and Giovanni NATILE. DNA adducts of antitumor trans-[PtCl2(E-imino ether)2]. Nucleic Acids Research. Oxford, UK: Oxford Press, 1996, No 24, p. 336-341. ISSN 0305-1048. info
• KAŠPÁRKOVÁ, Jana, K.J. MELISH, Yun QU, Viktor BRABEC and Nicholas FARRELL. Site-specific d(GpG) intrastrand cross-links formed by dinuclear platinum complexes. Bending and NMR studies. Biochemistry. Washington, DC, USA: American Chemical Society, 1996, No 35, p. 16705-16713. ISSN 0006-2960. info
• NIKI, Katsumi, Oldřich VRÁNA and Viktor BRABEC. Spectroelectrochemistry. In Bioelectrochemistry: Principles and Practice. Basel, Switzerland: Birkhäuser Verlag, 1996, p. 251-287. 3. ISBN 3-7643-5084-9. info
• VRÁNA, Oldřich, Vladimír BOUDNÝ and Viktor BRABEC. Superhelical torsion controls DNA interstrand cross-linking by antitumor cis-diamminedichloroplatinum (II). Nucleic Acids Research. Oxford, UK: Oxford Press, 1996, vol. 24, (20), p. 3918-3925. ISSN 0305-1048. info
• ŽALUDOVÁ, Renata, Vladimír KLEINWÄCHTER and Viktor BRABEC. The effect of ionic strength on melting of DNA modified by platinum(II) complexes. Biphysical Chemistry. Amsterdam: North-Holland Publishing Co, 1996, No 60, p. 135-142. ISSN 0301-4622. info
• KAŠPÁRKOVÁ, Jana and Viktor BRABEC. Recognition of DNA interstrand cross-links of cis-diamminedichloroplatinum(II) and its trans isomer by DNA- binding proteins. Biochemistry. Washington, DC, USA: American Chemical Society, 1995, No 34, p. 12379-12387. ISSN 0006-2960. info
• NOVÁKOVÁ, Olga, Jana KAŠPÁRKOVÁ, Oldřich VRÁNA, Paul M. VANVLIET, Jan REEDIJK and Viktor BRABEC. Correlation between cytotoxicity and DNA binding of polypyridyl ruthenium complexes. Biochemistry. Washington, DC, USA: American Chemical Society, 1995, No 34, p. 12369-12378. ISSN 0006-2960. info
• NOVÁKOVÁ, Olga, Oldřich VRÁNA, Valerie I. KISELEVA and Viktor BRABEC. DNA interactions of antitumor platinum(IV) complexes. European Journal of Biochemistry. New York, USA: Springer, 1995, No 228, p. 616-624. ISSN 0014-2956. info
• VAN VLIET, Paul M., S.M.S. TOEKIMIN, J.G. HAASNOOT, Jan REEDIJK, Olga NOVÁKOVÁ, Oldřich VRÁNA and Viktor BRABEC. mer-[Ru(terpy)Cl3] (terpy = 2,2':6',2''-terpyridine) shows biological activity, forms interstrand cross-links in DNA and binds two guanine derivatives in a trans configuration. Inorganica Chimica Acta. Oxford, UK: Elsevier Science, 1995, No 231, p. 57-65. ISSN 0020-1693. info
• BRABEC, Viktor, Vladimír BOUDNÝ and Zdeňka BALCAROVÁ. Monofunctional adducts of platinum (II) produce in DNA a sequence-dependent local denaturation. Biochemistry. Washington, DC, USA: American Chemical Society, 1994, vol. 33, (6), p. 1316-1322. ISSN 0006-2960. info
• BRABEC, Viktor and Vladimír BOUDNÝ. Monofunctional and interstrand DNA adducts of platinum (II) complexes. Metal-based Drugs. London, UK: Freud Publishing House Ltd., 1994, vol. 1, p. 195-200. ISSN 0793-0291. info
• LENG, Marc and Viktor BRABEC. DNA adducts of cisplatin, transplatin and platinum-intercalating drugs. In DNA Adducts: Identification and Biological Significance. Lyon, France: International Agency for Research on Cancer, 1994, p. 339-348. IARC Scientific Publications, No. 125. info
• TIMKOVSKII, Andrej L., Zdeňka BALCAROVÁ and Viktor BRABEC. Analysis of structural defects of poly(G).poly(C) complex. Molecular Biology. New York, USA: Consultants Bureau, 1994, No 28, p. 661-663. ISSN 0026-8933. info
• BRABEC, Viktor, Miroslav ŠÍP and Marc LENG. DNA conformational distortion produced by site-specific interstrand cross-link of trans-diamminedichloroplatinum(II). Biochemistry. Washington, DC, USA: American Chemical Society, 1993, No 32, p. 11676-11681. ISSN 0006-2960. info
• BRABEC, Viktor and Zdeňka BALCAROVÁ. Restriction enzyme cleavage of DNA modified by platinum(II) complexes. European Journal of Biochemistry. New York, USA: Springer, 1993, No 216, p. 183-187. ISSN 0014-2956. info
• BRABEC, Viktor and Marc LENG. DNA interstrand cross-links of trans-diamminedichloroplatinum(II) are preferentially formed between guanine and complementary cytosine residues. Proceeding of the National Academy of Sciences of the USA. Washington, DC, USA: National Academy of Sciences of the USA, 1993, No 90, p. 5345-5349. ISSN 0027-8424. info

2002/05/23