LF:BLKG051x Clinical Genetics - practice - Course Information

BLKG051x Clinical Genetics - practice

Extent and Intensity

0/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Michaela Gregorovičová (assistant)

Guaranteed by

doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová

Timetable

Mon 8:00–14:00 N05079, Tue 8:00–14:00 N05079, Wed 8:00–14:00 N05079, Thu 8:00–14:00 N05079

Prerequisites (in Czech)

BLKG0311 Clinical Genetics

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Laboratory Assistant (programme LF, B-SZ)

Course objectives

Specialist practice complements the practical part of education in Clinical Biochemistry, Clinical Hematology, Immunohematology and Transfusion medicine, Clinical Microbiology, Clinical Immunology, Clinical Genetics and Pathologic Anatomy – Histopathologic determination methods. Students become familiar with the organization of workflow processes and basic documentation of laboratory worksites and will perform select tasks and activities.; The aim of the course is to acquaint students with the operation in genetic laboratories, laboratory equipment and instrumentation procedures. Part of the course is visiting to the genetic clinic and get basic knowledge about genetic testing procedures.

Syllabus

• Methods of classical cytogenetics - Making preparations for the analysis of congenital and acquired chromosomal aberrations up. Making preparations for the analysis of chromosomal aberrations in cancer patiens up. Evaluation of chromosomal preparations under the light microscope and by computer image analysis. Methods of Molecular Cytogenetics - Method of fluorescence in situ hybridization, evaluation of preparations in the fluorescent microscope. Showing results of metaphases and interphase FISH. Methods CGH, SKY. Methods of Molecular Genetics - Taking of peripheral blood, DNA isolation methods, quality control DNA, RNA isolation. PCR amplification method. Detection of PCR products, electrophoresis in polyacrylamide gel. Methods of detection of mutations in clinical genetics (DGGE, SSCP, Real time PCR, fragmentation analysis, sequencing) Examination of patients in the genetic clinic

Literature

• FLODROVÁ, Eva, Alexandra ŽOURKOVÁ, Jan JUŘICA, Tomáš KAŠPÁREK and Renata GAILLYOVÁ. Molecular genetic analysis of CYP2D6 gene and used methods. In European Human Genetic Conference. 2008. URL URL info
• FLODROVÁ, Eva, Alexandra ŽOURKOVÁ, Jan JUŘICA and Renata GAILLYOVÁ. Molecular genetic identification of the major CYP2D6 alleles and utilization in psychiatric treatment. In 26th Collegium Internationale Neuro-Psychopharmacologicum Congress (CINP). 2008. ISSN 1461-1457. info
• VOJTÍŠKOVÁ, Marie, J. KADLECOVÁ, T. NOVOTNÝ, Renata GAILLYOVÁ, Hana KONEČNÁ, Jana KLÁNOVÁ and B. SEMRÁD. Molekulární diagnostika pacientů se syndromem prodlouženého QT intervalu (Molecular Diagnostics of Pacients with Long QT Syndromes). In Biochemie a molekulární biologie na prahu nového tisíciletí. 2000th ed. Brno: Masarykova univerzita, 2000, p. 83-84. ISBN 80-210-2266-3. info
• JIČÍNSKÁ, H., J. MAREK, Věra BRYŠOVÁ, Renata GAILLYOVÁ, Jiří LITZMAN, Petr KUGLÍK and Jiří NAVRÁTIL. Delece 22q11 chromozomu u vrozených srdečních vad (VSV) (Chromosome 22q11 deletion in congenital heart defects )CHD)). Česko-slovenská pediatrie. Praha: ČLS, 1998, vol. 53, suppl. 1, p. 67. ISSN 0069-2328. info

Teaching methods

trainee - ship

Assessment methods

confirmation of completion of practice

Language of instruction

Czech

• Enrolment Statistics (Autumn 2009, recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2009/BLKG051x