MNAB0711s Applied Biochemistry

Faculty of Medicine
Autumn 2013
Extent and Intensity
0/2. 3 credit(s). Type of Completion: k (colloquium).
prof. MUDr. Vladimír Soška, CSc. (lecturer)
MVDr. Halina Matějová (assistant)
Guaranteed by
prof. MUDr. Vladimír Soška, CSc.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Mon 30. 9. to Sun 8. 12. Mon 14:00–16:30 B11/114
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After this course the participant should be able to: - choose biochemical tests suitable for diagnostics of diseases - know the basic problems of common biochemical diagnostic tests - know how to collect biological material and how to treat it to prevent false results - to assess reliability of laboratory results.
  • Analytical sources of variation, accuracy and precision. Diagnostic accuracy, sensitivity, specificity, and efficiency of diagnostic tests. Reference ranges, decision values. Collection and processing of specimens, special requirements, general safety rules.
  • Assessment and monitoring of nutritional status. Blood plasma proteins. Acute-phase reactants. Principles of nutritional support, enteral and parenteral nutrition. Dietary constituents providing a sufficient energy intake.
  • Energy output, nitrogen balance. Indications for nutritional support. Oxygen, transport and metabolism, tissue hypoxia.
  • Disorders of lipid and lipoprotein metabolism.
  • Ischemic heart disease, congestive cardiac failure. Potassium and magnesium homeostasis.
  • Iron and essential microelements metabolism.
  • Disturbances of water and sodium balance. Plasma and urine osmolality. Plasma electrolytes in acid-base disorders.
  • Assessment of acid-base balance. Investigating an acid-base status, indications for blood acid-base and oxygen measurements, interpretation of results.
  • Laboratory evaluation of kidney function. Biochemical monitoring of acute and chronic renal failure, and of patients receiving haemodialysis. Urine sediment.
  • Investigation of liver and biliary tract diseases. Signs of the liver injury, differentiating of hyperbilirubinaemias, syndrome of biliary obstruction.
  • Clinical biochemistry of the GIT. Gastric acid secretion, maldigestion and malabsorption, assessment of pancreatic function.
  • Disorders of saccharide metabolism, diabetes mellitus.
  • Calcium and phosphate metabolism. Biochemical markers of bone metabolism (in the assessment of osteoporosis).
  • Uric acid, hyperuricaemia. Renal tract stones.
  • Laboratory assessment of thyroid and adrenal functions.
  • Tumour markers. Biological causes of variation of test results, effects of drugs on chemical tests.
    required literature
  • RACEK, Jaroslav. Klinická biochemie. 2., přeprac. vyd. Praha: Galén, 2006. 329 s. ISBN 8072623249. info
    recommended literature
  • DASTYCH, Milan. Klinická biochemie - bakalářský obor Zdravotní laborant (Clinical Biochemistry). 1. vydání. Brno: Masarykova univerzita, 2008. 232 pp. ISBN 978-80-210-4572-9. info
  • MASOPUST, Jaroslav. Klinická biochemie : požadování a hodnocení biochemických vyšetření. Část I. Vyd. 1. Praha: Karolinum, 1998. 429 s. ISBN 80-7184-648-11. info
    not specified
  • Clinical chemistry : techniques, principles, correlations. Edited by Michael L. Bishop - Edward P. Fody - Larry E. Schoeff. 6th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins, 2010. xx, 732. ISBN 9780781790451. info
  • JABOR, A. Vnitřní prostředí. 1st ed. Praha: Grada Publishing,a.s., 2008, 2009. 560 pp. ISBN 978-80-247-1221-5. info
  • Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis, Mo.: Saunders/Elsevier, 2008. xx, 952. ISBN 9780721638652. info
Teaching methods
Assessment methods
oral exam
Language of instruction
Follow-Up Courses
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021.
  • Enrolment Statistics (Autumn 2013, recent)
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