FDGPM_FAF Separative and Analytical Methods

Faculty of Pharmacy
Autumn 2020
Extent and Intensity
1/0/0. 25 credit(s). Type of Completion: zk (examination).
PharmDr. Dagmar Jankovská, Ph.D. (lecturer)
prof. PharmDr. Karel Šmejkal, Ph.D. (lecturer)
Ing. Emil Švajdlenka, Ph.D. (lecturer)
Guaranteed by
prof. PharmDr. Karel Šmejkal, Ph.D.
Faculty of Pharmacy
The knowledge of basics of organic chemistry, inorganic chemistry, physical chemistry and phytochemistry
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The Separation Analysis Methods aim to show DSP students modern methods of isolation of natural substances, especially chromatographic techniques. It deepens knowledge of both analytical and preparative separation of secondary metabolites, focusing on specific methods of division and detection. It shows the possibilities of so-called scale-up chromatographic systems, the specifics in the division of different types of substances, and the possibility of verifying the quality of the separation performed.
Learning outcomes
The ability to practically use all the available separation analytical methods, to control their theoretical basics.
  • Separation analytical methods is a subject showing the modern approaches for the isolation of natural compounds, namely using different chromatographic techniques. It shows possibilities of both analytical and preparative separation, including the scaling up of the methods. It is targeted on specifics of separation of different compounds, chromatographic systems, and also on the control of the quality of the separation process.
    1) analytical HPLC
    2) semipreparative HPLC
    3) analytical TLC
    4) preparative TLC
    5) column chromatography
    6) further separation techniques
    recommended literature
  • Smejkal, K, Muselik, J, Mokry, P. Laboratory methods of experimental phytochemistry. Brno, 2017. ISBN 978-80-7305-796-1. info
  • Sarker, SD, Latif, Z, Gray, AI. Natural Products Isolation. New jersey, 2006. ISBN 1-59259-955-9. info
  • Walton, NJ, Brown, DE. Chemicals from Plants. London, 1999. ISBN 981-02-2773-6. info
Teaching methods
Discussion, reading, brainstorming, demonstration of methods.
Assessment methods
Oral examination
Language of instruction
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught each semester.
Teacher's information
Basics of separation techniques
Consultation with teacher
Practical application of acquired knowledge
The course is done using self-studies, personal communication, and consultations with the lecturer
The course is also listed under the following terms Spring 2020, Spring 2021, Autumn 2021, Spring 2022.
  • Enrolment Statistics (Autumn 2020, recent)
  • Permalink: https://is.muni.cz/course/pharm/autumn2020/FDGPM_FAF