C8160 Enzymology

Faculty of Science
Autumn 2020
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught online.
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
Tue 8:00–9:50 Pouze distančně
C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry, organic chemistry and physical chemistry are recommended.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims at providing detailed background information on the most important aspects of enzymatic reactions including structure of enzymes, enzyme activity measurement, enzyme purification, reaction mechanisms, kinetics, and bioanalytical and industrial applications of biocatalysis.
Learning outcomes
The student will get a basic idea about the properties of enzymes and the relationship between their structure and mechanisms of catalytic action. He/she will know the most important representatives of individual classes of enzymes, their use in practice and the most common methods for the determination of enzyme aktivity and substrate concentration. He/she also will be able to process data from kinetic studies and interpret the results in relation to the reaction mechanism.
  • 1) First sight into the world of enzymes: structure, specifity, nomenclature. 2) Enzyme activity and their measurement. 3) Isolation of enzymes from the natural sources and expression systems. 4) Enzyme catalysis from the point of view of organic chemistry. 5) Enzyme catalysis from the point of view of physical chemistry. 6) Basic theoretical concepts in enzyme kinetics. 7) Effects of environmental factors (pH, temperature) on enzymes. 8) Reversible and irreversible inhibition of enzymes. 9) Kinetics of multisubstrate enzyme reactions. 10) Cooperativity in enzyme function. 11) Immobilized enzyme systems. 12) Enzymes as tools in bioanalysis. 13) Use of enzymes in technology and medicine. Enzyme engineering.
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994. 152 s. ISBN 8021010398. info
  • KOTYK, Arnošt and Jaroslav HORÁK. Enzymová kinetika. 1. vyd. Praha: Academia, 1977. 268 s. info
  • PRICE, Nicholas C. and Lewis STEVENS. Fundamentals of enzymology : the cell and molecular biology of catalytic proteins. 3rd ed. Oxford: Oxford University Press, 1999. xviii, 478. ISBN 019850229X. info
  • BISSWANGER, Hans. Practical enzymology. 2nd, completely rev. ed. Weinheim: Wiley-Blackwell, 2011. xvi, 360. ISBN 9783527320769. info
  • LESKOVAC, Vladimir. Comprehensive enzyme kinetics. New York: Kluwer Academic/Plenum publishers, 2003. xi, 438. ISBN 0306467127. info
  • CORNISH-BOWDEN, Athel. Fundamentals of enzyme kinetics. Rev. ed. London: Portland Press, 1995. xiii, 343. ISBN 1855780720. info
Teaching methods
Assessment methods
A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.
Language of instruction
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019.
  • Enrolment Statistics (recent)
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