Bi7201 Genomics - a basic course

Faculty of Science
Autumn 2004
Extent and Intensity
1/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
RNDr. Eliška Nejedlá, CSc. (seminar tutor)
RNDr. Alena Kuderová, CSc. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Mgr. Eva Paděrová (seminar tutor)
Mgr. Markéta Pernisová, Ph.D. (assistant)
Nagavalli Subbanna Kiran, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Břetislav Brzobohatý, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Břetislav Brzobohatý, CSc.
Prerequisites
Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
Course objectives
Students will acquire a working knowledge of genomic databases and their applications in experimental analysis of biological functions of genes and genomes. Biological function of a concrete member of a complex gene family will be analysed by identification of an insertion mutation in the gene and analysis of phenotype alterations in the mutant.
Syllabus
  • Introduction to genomics. Methods of functional genomics. Genome databases. Insertion mutant collections - a powerful tool for plant functional genomics. DNA fragment analysis - automated genetic analyzer ABI PRISM 310. Oligonucleotide design, synthesis and purification - EXPEDITE 8909 Nucleic Acid Synthesizer. Genomic DNA isolation from Arabidopsis thaliana. PCR, DNA electrophoresis in agarose gels. Southern blot and DNA molecular hybridization. Identification and characterization of an insertion mutation in a selected member of a complex gene family in Arabidopsis thaliana via a PCR based screen.
Literature
  • Hunt, S.P., Livesey, F.J. (Editors). Functional Genomics : A Practical Approach. Practical Approach Series
  • Starkey, M.P., and Elaswarapu R. (Editors). Genomics Protocols. Methods in Molecular Biology, Vol 175
Assessment methods (in Czech)
blokové praktikum, ukončeno (klasifikovaným) zápočtem
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: 1 týden.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012.
  • Enrolment Statistics (Autumn 2004, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2004/Bi7201