CS

PřF:C7187 Experimental oncology - Course Information

C7187 Experimental oncology

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Bouchal, Ph.D. (lecturer)
doc. Mgr. Roman Hrstka, Ph.D. (lecturer)
MUDr. Petr Müller, Ph.D. (lecturer)
MUDr. Rudolf Nenutil, CSc. (lecturer)
RNDr. Bořivoj Vojtěšek, DrSc. (lecturer)
Supervisor
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Prerequisites
C4182 Biochemistry II || Bi4020 Molecular biology
This course is suitable for students of master, doctoral and of 3rd year of bachelor study in biochemistry and molecular biology. The knowledge at biochemisty and molecular biology basic courses level is required.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
Fields of study the course is directly associated with
there are 15 fields of study the course is directly associated with, display
Course objectives
At the end of the course, the students will understand the basics of biology and biochemistry of tumor cells as well as the mechanisms of malign transformation and spreading of tumors. They should be able to apply genomics and proteomics approaches and to use the main database sources in experimental oncology. The students should be able to explain the approaches used in production and purification of proteins and antibodies, principles of moleclar cloning, gene therapy and the use of therapeutical antibodies. They should be able to make decisions about the proper use of biological material like model systems (cell lines), human tissues, or human plasma. The lecturers will provide insights into oncological pharmacology including drug discovery and testing, and into the basics of the use of viruses in experimental oncology. They will focus one of the lessons on the aspects of cooperation and carrier in science in general.
Syllabus
  • 1. Introduction, organization of the course (Dr. Bouchal). Cooperation and carrier in the science (Dr. Vojtěšek).
  • 2. Physical, chemical and biological factors of carcinogenesis (Dr. Bouchal): Physical radiation, mechanisms of chemical carcionogens, tests of mutagenity, viral transformation. Morphological and biochemical changes in transformed cells, hypoxia. Mechanisms of cytostatic agents action, drug resistance. Examples of clinically used tumor markers.
  • 3. Introduction in tumor cell biology (Dr. Hrstka). Mechanisms of malign transformation. Angiogenesis, formation of metastases. Molecular biomarkers.
  • 4. Important signal pathways in the tumor cell. Tumor epidemiology. (Dr. Hrstka). p53 protein, potential use in the therapy focused on the cell cycle.
  • 5. Human genome mapping, functional genomics, epigenetics. (Dr. Hrstka). PCR based methods, chip based methods, new generation sequencing. Gene engineering. Model systems in applied research.
  • 6. Proteomic approaches in experimental oncology (Dr. Bouchal). Protein 2-D gel elekctrophoresis, DIGE, protein identification via mass spectrometry. Quantitative proteomic approaches based on peptide MS, stable isotope labeling (SILAC, iTRAQ). MRM as a tool of targeted proteomics. Proteomic experiment in experimental oncology: The choice of biological material, study design, statistical analysis, data validation and interpretation.
  • 7. Genomics and proteomics database sources in experimental oncology (Dr. Bouchal, Dr. Hrstka). Practical use of the algorithms: NCBI, ExPASy, UniProt, NCBI, BLAST, secundary structure and posttranslation modification prediction, Human Protein atlas.
  • 8. Gene therapy (Dr. Müller). Diseases curable by gene therapy. Vectors for gene therapy. Perspectives of gene therapy. Viruses and their use in experimental oncology. Characterization and categories of viruses. Tumors and viruses. Adenovirus and lentivirus vectors. Viruses and gene therapy.
  • 9. Protein engineering (Dr. Müller). Protein-protein interactions, „phage display“. Analysis of the activity. „Protein engineering“
  • 10. Protein and expression and purification (Dr. Vojtěšek). The use of expression systems for protein expression. The use of „Tag sequences“. Protein preparation for production of monoclonal antibodies. Protein preparation for functional studies.
  • 11. Design, production and characterization of antibodies (Dr. Vojtěšek). Immunization schema, suitable animals and antigens. Production, purification, characterization and labeling of monoclonal and polyclonal antibodies. Application and humanization of antibodies.
  • 12. The use of human biological material in research (Dr. Nenutil). Human tissues as a source of information and a tool for validation of data obtained from experimental models. The use and processing of tissue samples. Predictive and prognostic pathology. Biological characteristics of tumors and their prognosis. Biological predictors of therapeutical response.
  • 13. Drug development (Dr. Müller). Therapeutical substance, pharmacodynamics and pharmacokinetics. The models used for „drug screening“. Databases of chemical substances versus natural products. Quantitative and structural analysis of the potential therapeutical substance. Rational drug development. Clinical studies.
Literature
  • REJTHAR, Aleš and Bořivoj VOJTĚŠEK. Obecná patologie nádorového růstu. 1. vyd. Praha: Grada, 2002. 206 s. ISBN 802470238X. info
Teaching methods
Presentations by professionals followed by class discussion and answers to students' questions. Practical use of database sources.
Assessment methods
Written exam followed by a short discussion with the lecturer.
Language of instruction
Czech
The course is also listed under the following terms Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.