PřF:Bi1700c Cell Biology - practice - Course Information
Bi1700c Cell Biology - practical courseFaculty of Science
- Extent and Intensity
- 0/1/0. 1 credit(s). Type of Completion: z (credit).
- MVDr. Mgr. Monika Dušková, Ph.D. (seminar tutor)
doc. RNDr. Jakub Neradil, Ph.D. (seminar tutor)
RNDr. Jan Škoda, Ph.D. (seminar tutor)
RNDr. Petra Celá, Ph.D. (seminar tutor)
Mgr. Ivana Kupčíková, DiS. (assistant)
- Guaranteed by
- prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: MVDr. Mgr. Monika Dušková, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- Timetable of Seminar Groups
- Bi1700c/01_1: Tue 12:00–13:50 C13/332, J. Neradil
Bi1700c/01_2: Tue 12:00–13:50 C13/332, J. Neradil
Bi1700c/02_1: Tue 14:00–15:50 C13/332, J. Neradil
Bi1700c/02_2: Tue 14:00–15:50 C13/332, J. Neradil
Bi1700c/03_1: Wed 13:00–14:50 C13/332, J. Neradil
Bi1700c/03_2: Wed 13:00–14:50 C13/332, J. Neradil
Bi1700c/04_1: Wed 11:00–12:50 C13/332, J. Škoda
Bi1700c/04_2: Wed 11:00–12:50 C13/332, J. Škoda
Bi1700c/05_1: Tue 17:00–18:50 BR1, M. Dušková
Bi1700c/05_2: Tue 17:00–18:50 BR1, M. Dušková
Bi1700c/06_1: Thu 11:00–12:50 BR1, P. Celá, M. Dušková
Bi1700c/06_2: Thu 11:00–12:50 BR1, P. Celá, M. Dušková
Bi1700c/07_1: Thu 7:00–8:50 BR1, M. Dušková
Bi1700c/07_2: Thu 7:00–8:50 BR1, M. Dušková
Bi1700c/08_1: Thu 9:00–10:50 BR1, P. Celá, M. Dušková
Bi1700c/08_2: Thu 9:00–10:50 BR1, P. Celá, M. Dušková
- Prerequisites (in Czech)
- NOW ( Bi1700 Cell Biology )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 21 fields of study the course is directly associated with, display
- Course objectives
- The practical course of Cell Biology consists of six thematic blocks of practical tasks, taking up the lecture of Cell Biology. The topics of the practical tasks involve issues such as the transport of substances across the cell membrane, cell locomotion and irritability, mitosis, meiosis, training with light microscope as well as basic laboratory procedures employed in biology laboratories. After this course, students should be able to work with automatic pipettes, to prepare solutions, to know the correct procedures for microscopy, and to prepare simple plant or animal microscopy slides. The course has been designed as a preparation for more specialized courses and is aimed to harmonize unequal levels of students' knowledge from secondary schools.
- 1. Measuring exact volumes in biology. Manipulation with automatic pipettes and pipetting heads or with glass pipettes: repeated (10 times) pipetting an exact volume of the liqiud, weighing and statistical assessment of the precision of laboratory work. Model calculations of solution concentration 2. Manipulation with light microscope, training with immersion objective, measuring biological objects using a stage micrometer and a reticle. Native preparations of parenchyma cells from the Elodea waterweed, permanent slides od blood smears, measuring individual types of blood cells: erythrocytes, leukocytes, trombocytes from humans and from other class of vertebrates. 3. Transport of substances, osmotic phenomena. Plasmolysis and deplasmolysis in epidermal cells of onion and the Elodea waterweed, dependence of reversibility of this process on the concentration of plasmolytic solution. Observation of pollen grains in hypotonic solutin, reaction of erytrocytes in hyper- and hypotonic solutions. 4. Mitosis, modification of mitosis (polyploidy, polyteny). Mitotic figures in onion bulb cells, temporary and permanent slides, staining with aceto-orcein. Mitotic failures – onion bulb after hydrochinon treatment, temporary slide stained with aceto-orcein. Counting the mitotic index in permanent slides of tissue cultures. Preparation of salivary glands from Chironomidae, comparison of temporary and permanent slide, polyploid nuclei of the silkworm (Bombyx mori). 5. Meiosis. Permanent slides of the ovary and seminiferous tubules, egg cells from the Pelophylax frog, pigs' sperm cells – live spermatozoa and permanent slides. 6. Cell locomotion and irritability. Brownian motion observed in a suspension of ferric oxide particles. Observation of protozoa in a hay infusion – ciliary, amoeboid and flagellar motion. Chemotaxis of protozoa from a solution drop containing NaCl into a drop of pure water through a thin liquid bridge. Oxygenotaxis of protozoa around an air bubble.
- recommended literature
- PALEČEK, Jiří. Biologie buňky. 1. vyd. Praha: Karolinum, 1996. 120 s. ISBN 8071842664. info
- KOPECKÁ, Marie. Lékařská biologie. Praktická cvičení - část druhá : genetika. 1. vyd. Brno: Vydavatelství Masarykovy univerzity, 1995. 70 s. ISBN 80-210-1072-X. info
- PALEČEK, Jiří. Obecná zoologie :praktická cvičení. Vyd. 2. Praha: Univerzita Karlova, 1991. 223 s. ISBN 80-7066-493-2. info
- not specified
- KOPECKÁ, Marie. Lékařská biologie : praktická cvičení. 1. vyd. Brno: Masarykova univerzita, 1995. 70 s. ISBN 802101072X. info
- Teaching methods
- laboratory practice, microscopic observation
- Assessment methods
- oral exam, protocols
- Language of instruction
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on the extent and intensity of the course: každý druhý týden 2 hodiny.
- Listed among pre-requisites of other courses