PřF:C7350 MS of proteins - practice - Course Information

C7350 Protein characterisation using mass spectrometry - practice

Extent and Intensity

0/1/0. 1 credit(s) (plus extra credits for completion). Recommended Type of Completion: z (credit). Other types of completion: graded credit.

Teacher(s)

prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)

Guaranteed by

prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 8:00–9:50 C02/211

Prerequisites (in Czech)

NOW ( C7250 Mass spectrometry of proteins )

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Analytical Biochemistry (programme PřF, N-BCH)
• Analytical Chemistry (programme PřF, N-CH)
• Biophysics (programme PřF, D-FY4)
• Biophysics (programme PřF, N-FY)
• Biochemistry (programme PřF, D-BCH4)
• Biochemistry (programme PřF, N-BCH)
• Biomolecular Chemistry (programme PřF, D-BCH4)
• Biomolecular Chemistry (programme PřF, N-BCH)
• Microbiology (programme PřF, D-BI4)
• Molecular and Cellular Biology (programme PřF, D-BI4)
• Molecular Biology and Genetics (programme PřF, N-EXB)
• General and Molecular Genetics (programme PřF, D-BI4)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Ekotoxikologie)
• Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
• Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
• Special Biology (programme PřF, N-EXB, specialization Mikrobiologie a molekulární biotechnologie)

Course objectives

At the end of the course students should be able to: understand basics of protein identification based on MS a MS/MS data;
select and use correct search software for identification of proteins;
understand basics of interpretation of MS/MS spectra;

Syllabus

• Interpretation of MS data;
• Interpretation of MALDI-MS/MS and ESI-MS/MS data;
• Protein identification based on MS and MS/MS data;
• Introduction into de novo sequencing;
• Characterisation of protein modification - determination of type and site;

Literature

recommended literature
• SNYDER, Peter A. Interpreting protein mass spectra :a comprehensive resource. Oxford: Oxford University Press. xi, 522 s. ISBN 0-8412-3571-6. 2000. info

Teaching methods

practical work with data using PC

Assessment methods

individual processing of test data

Language of instruction

Czech

Further Comments

The course is taught annually.

• Enrolment Statistics (Autumn 2014, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2014/C7350