CS

PřF:Bi5599 App.Chem.and Biochem. - Course Information

Bi5599 Applied Chemistry and Biochemistry

Faculty of Science
autumn 2017
Extent and Intensity
2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Jan Vondráček, Ph.D. (lecturer)
RNDr. Miroslav Machala, CSc. (lecturer)
Supervisor
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Jan Vondráček, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Mon 9:00–10:50 A11/333
Prerequisites (in Czech)
Bi4020 Molecular biology && C3580 Biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
Fields of study the course is directly associated with
Course objectives
The main goal of the course is to enable students to understand the importance of individual chemical/biochemical techniques and to interpret both their own experimental data, based on the techniques taught in the course. They should be able to work with the information in the primary literature, in order to understand and implement the techniques described in primary scientific literature in the area of animal physiology and molecular physiology. Based on this, they will be able to present and explain the techniques used in their own work to expert audience.
Learning outcomes
At the end of the course, students will be able to: identify and describe basic methodology for determination of protein levels and localization, estimation of protein-protein interactions, functional analysis of protein domains, interactions of proteins with DNA; describe basic principles of manipulation of protein expression and function; describe principles of isolation of nucleic acids and determination of gene expression; describe methodology for analyses of lipids and small organic molecules based on chromatography and mass spectrometry; describe basic chemical and biochemical methods used in primary literature.
Syllabus
  • 1)Protein analysis; separation techniques; Western blotting, protein-protein interactions; protein localization; protein post-translational modifications; proteomics; manipulations of protein expression.
  • 2)Basic immunochemistry techniques.
  • 3)DNA isolation, separation; PCR techniques, plasmid work, transfection of eukaryotic cells.
  • 4)RNA isolation, detection (Northern blotting, RT-PCR), microarrays, siRNA.
  • 5)Lipids and polysaccharides; isolation, separation and identification.
  • 6)Low-molecular-weight compounds - separation and analytical techniques; HPLC techniques and their modifications.
  • 7)Specific cellular assays.
  • 8)Fluorescence techniques.
  • 9)Application of in vivo mammalian models in physiology.
Literature
  • Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
  • Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000
  • Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
Teaching methods
lectures, class discussion
Assessment methods
The course is finished as colloquium. Each student is required to prepare a short presentation (10 minutes) about methodical approaches used in an assigned scientific paper. Students summarize in their presentation both foundations and results of the assigned paper, and tehy commentin deatil the methodology used - a specific method is described by them according to the selection made by lecturers. Final evaluation is based on the quality of presentation and on the outcome of discussion with lecturers.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018.
  • Enrolment Statistics (autumn 2017, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2017/Bi5599