PřF:C8170 Enzymology-sem. - Course Information
C8170 Enzymology - seminarFaculty of Science
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- prof. RNDr. Igor Kučera, DrSc. (lecturer)
- Guaranteed by
- prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
- Mon 18. 9. to Fri 15. 12. Mon 15:00–16:50 B11/305
- NOW ( C8160 Enzymology )
related to the course on Enzymology
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Teaching methods
- seminars, and practical training related to the content of the Enzymology lecture course, calculations
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Further Comments
- Study Materials
The course is taught annually.