PřF:C7041 Molecular Spectrometry - Course Information
C7041 Molecular SpectrometryFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- prof. RNDr. Viktor Kanický, DrSc. (lecturer)
doc. Mgr. Petr Táborský, Ph.D. (lecturer)
- Guaranteed by
- prof. RNDr. Viktor Kanický, DrSc.
Department of Chemistry - Chemistry Section - Faculty of Science
- Tue 16:00–17:50 A8-309
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Analytical Chemistry (programme PřF, M-CH)
- Analytical Chemistry (programme PřF, N-CH)
- Inorganic Chemistry (programme PřF, M-CH)
- Biochemistry (programme PřF, M-CH)
- Physical Chemistry (programme PřF, M-CH)
- Chemistry (programme PřF, M-CH)
- Environmental Chemistry (programme PřF, M-CH)
- Environmental Chemistry (programme PřF, N-CH)
- Macromolecular Chemistry (programme PřF, M-CH)
- Organic Chemistry (programme PřF, M-CH)
- Course objectives
- Classification of methods, analytical and structural aspects; wavelength ranges and procesess in the species. Instrumentation, monochromatization, signal treatment. Molecular absorption spectrophotometry UV/VIS, Bouguer-Lambert-Beer's law, multicomponent analysis, photoacoustic spectrometry. Luminiscence, laser fluorimetry. Diffuse radiation scatter. Infrared and Raman spectrometry. Microwave spectrometry. Analytical aspects of magnetic resonance methods. Analytical refractometry and interferometry.
- 1. Classification of optical analytical methods, overview of methods of molecular spectroscopy, analytical and structural aspects of optical methods, radiation - matter interactions. Photometry and units. 2. Molecular absorption spectroscopy in UV and Vis spectral ranges: nature and character of UV and Vis spectra, molecular orbitals, symbols of molecular terms, multiplicity, electronic states of molecules. 3. Types of electronic transitions and their expressions in spectra, chromophores, auxochromes, shape and structure of absorption bands, vibronic transitions. 4. Electronic spectra of important classes of compounds: aliphatic unsaturated hydrocarbons, derivatives of aliphatic hydrocarbons, aromatic hydrocarbons, their heteroanalogues and substitute derivatives, organic dyes, inorganic ions and complexes of metal cations,, charge-transfer complexes. 5. Extrinsic and intrinsic effects influencing electronic spectra: steric effects, tautomeric equilibria, pH, solvents. Empiric calculations of electronic spectra. Instrumentation of UV/Vis absorption spectroscopy. 6. Applications of UV/Vis absorption spsectroscopy: structure of orgaic compounds, qualitative analysis. Bouguer-Lambert-Beer s law, quantitative analysis. Analytical use of scatter of light: turbidimetry, nephelometry, diffusion reflectance, titration variants of optical methods. 7. Luminiscence spectroscopy: nature and classification. Fluorimetry, phosphorimetry, relation structure-spectrum, analytical applications. Electro-, bio-, thermo-, chemiluminiscence, luminiscence in solid state (candoluminiscence), laser fluorimetry. Analytical applications. 8. Infrared spectroscopy: nature and character of infrared spectra, molecular vibrations and origin of vibrational spectra, rotational levels of molecules and rotational spectra, rotational-vibrational spectra, selection rules, intensity of absorption bands, vibrational frequencies and properties of molecules. 9. Factors influencing characteristic vibrations: state of matter, solvents, hydrogen bond, masses of atoms, electric charge effect, steric effects, cycle tension, conformation, vibrational interactions. Infrared spectra of organic compounds. Instrumentation and working techniques. 10. Raman spectroscopy, nature and character of Raman spectra, instrumentation, working technique, applications. Microwave spectroscopy: principle, instrumentation, applications. 11. Analytical applications of infrared and Raman spectroscopy. 12. Magnetic resonance spectroscopy: nuclear magnetic resonance spectroscopy, nature of NMR spectra, instrumentation, chemical shift, intensity of resonance signals, splitting of signals, 1st order spectra, spin systems, higher order spectra, chemical exchange. 13. NMR spectra of heavier atoms, applications of NMR spectroscopy. Spectroscopy of electron paramagnetic resonance. 14. Analytical refractometry. Optical rotation dispersion, circular dichroism. Moessbauer spectroscopy. Photoacoustic spectroscopy.
- Teaching methods
- Assessment methods
- lectures, oral examination
- Language of instruction
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.