PřF:C5991 Applied enzymology - seminars - Course Information
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2016
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 13:00–14:50 C05/114
- Prerequisites
- NOW ( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
- Enrolment Statistics (Spring 2016, recent)
- Permalink: https://is.muni.cz/course/sci/spring2016/C5991