(1) Lubomira Anderkova Introduction: Repetitive transcranial magnetic stimulation (rTMS) is a noninvasive tool for modulating cortical activity. [INS: :INS] Objectives: We[JM1] studied [INS: the :INS] effects of high frequency rTMS applied over the right inferior frontal gyrus (IFG) and the right superior temporal gyrus (STG) on cognitive functions in patients with amnestic mild cognitive impairment (MCI) and incipient dementia [DEL: due to :DEL] [INS: caused by :INS] Alzheimer´s disease (AD). [INS: :INS] Methods: Ten patients (6 men; 4 women, mean age 72 ± 8 years; MMSE 23 ± 3.56) were enrolled in the randomized, placebo-controlled study with a crossover design. All participants underwent 3 sessions of 10 Hz rTMS over the non-dominant right hemisphere (2250 stimuli/day, intensity 90% of motor threshold) in a random order: 1. IFG (an active stimulation site)[INS: ; :INS] [DEL: , :DEL] 2. STG (an active stimulation site[DEL: ), :DEL] [INS: ); :INS] 3. vertex (a control stimulation site). The Trail Making Test (TMT), the Stroop test and the visual memory encoding task (VMET) were administered before and immediately after each session. The Wilcoxon paired test was used for data analyses. [INS: :INS] Results: Only the stimulation applied over the IFG induced significant improvement in the TMT part A (p = 0.037) and B (p = 0.049). No significant changes were found in the Stroop test and in the VMET after the IFG stimulation. Moreover, we observed [JM2] no significant cognitive after-effects of rTMS applied over the STG and/or vertex. [INS: :INS] Conclusions: High frequency rTMS of the right IFG induced significant improvement of attention and psychomotor speed in patients with MCI and dementia [DEL: due to :DEL] [INS: caused by :INS] AD. This is an interim analysis of an ongoing study. (2) Andrea Belanova When Messiah Dies: Unification Church Members’ Perceptions of Reverend Moon’s Death Abstract of [INS: a :INS] [DEL: the :DEL] Conference Paper Th[INS: is :INS] [DEL: e :DEL] analysis focuses on the reactions of the Unification Church members to the death of the[INS: ir :INS] leader, and to the articles that appeared in the media [DEL: and :DEL] [INS: which :INS] referred to this event. Reverend Sun Myung Moon, the founder of [INS: a :INS] new religious movement[INS: , the :INS] Unification Church, died at the age of 92 in South Korea in September 2012. Surprisingly, his death was received by the church members with relative calm[DEL: mind :DEL] . They smoothly organized the funeral ceremony and the officials claimed that his death [INS: should be :INS] [DEL: is :DEL] understood only as a transformation into another state of being, and [DEL: it :DEL] thus should be celebrated. However, Moon’s death also attracted a lot of media attention, mostly in [INS: a :INS] [DEL: the :DEL] negative sense. The emotions were suddenly churned up around the death of the man, who claimed himself as Messiah and True Father of mankind. Considering [DEL: the :DEL] data [INS: that :INS] includ[INS: ed :INS] [DEL: ing :DEL] specifically interviews and written texts, I conducted a case study using [DEL: the methods of :DEL] qualitative sociology [INS: methods :INS] to investigate this unique situation. In my paper I argue that the reactions of the members are ambivalent in nature depending on the further context. Although Moon’s death is viewed as [DEL: a :DEL] “not a sad event” [DEL: by the :DEL] [INS: according to :INS] Unificationist[DEL: s :DEL] [DEL: according to the :DEL] theology[INS: , :INS] [DEL: ; :DEL] they demand respect and compassion for their grieving at the same time when it concerns media attention. The inner life of the church and the public image are thus understood as two different worlds where different emotions should be presented. The analysis shows how the “sacred” aspect may be protected from the profane world by[DEL: the :DEL] religious movements. (3) Jana Pazurikova Molecular dynamic[DEL: s :DEL] simulations of longer simulation[DEL: s :DEL] times [JM3] (~ tens of μs and more) capture events of great chemical and biological interest. Parallel-in-space runs of these simulations hit the strong scalability wall[INS: and :INS] [DEL: — :DEL] adding more processors does not shorten the time to results. Inspired by gravitational N-body simulations, we propose [JM4] [INS: an :INS] [DEL: the :DEL] algorithm calculating molecular dynamics parallel in time[JM5] . Our implementation of parareal method combined with multilevel summation method for evaluation of long-range interactions achieved almost perfect strong scaling up to half a million cores. Thus, compared to the simulation sequen[INS: ce :INS] [DEL: tial :DEL] in time, we reduced the wallclock[JM6] time of simulation by an order of magnitude. Shortening the calculations can push the research in medicine and pharmacy further as it condenses the time from an idea to the evaluation of [INS: a :INS] simulated experiment. (4) Martin Jirusek Strategic and market-oriented approaches to energy policy in policy documents of the Czech Republic and European Union The EU has been gradually increasing the emphasis on [INS: a :INS] common approach to energy security among its members [DEL: in :DEL] [INS: over the :INS] last couple of years and these states had to reflect this tendency in their own approach to energy policy. Additionally, both EU and its members were forced to react on several challenges regarding energy security. Reactions and tendencies within energy policies could be assessed on the basis of theoretical approaches that can not only clarify reasons for implementing specific tools and procedures, but also can help to anticipate future development. [INS: :INS] This article is focused on [INS: the :INS] energy policy of the Czech Republic and European Union from the perspectives of strategic and market-oriented approach[INS: es :INS] [INS: , :INS] which are the two dominant theoretical approaches in this field of study. The aim was to identify how these approaches are reflected in policy documents on both levels during the selected period of time (2004-2012) and what [DEL: does :DEL] it mean[INS: s :INS] in terms of energy policy coordination within the EU. (5) Monika Schön This essay deals with the matters of education of a child and a possible conflict of opinions among the persons who realize [JM7] the education of a child[INS: . This is discussed :INS] [DEL: , :DEL] in[DEL: the :DEL] light of the Convention for the Protection of Human Rights and Fundamental Freedoms (hereinafter „Convention“), before all art. 8, art. 9 of the Convention and art. 2 of the Protocol No. 1.[INS: [JM8] :INS] [INS: :INS] The essay [DEL: does :DEL] not [INS: only :INS] focus[INS: es :INS] [DEL: only :DEL] on the right to education which belongs to the child´s parents, [INS: and :INS] eventually to other persons (such as [INS: the :INS] state), but also on the right to education which belongs to the child and on the interest of a child to be educated. (6) Petr Dvorak Institutional Responsiveness in Parliamentary Democracies: [INS: A :INS] Longitudinal Study Th[INS: is :INS] [DEL: e :DEL] paper proposes a research framework to analyse institutional responsiveness and parliamentary regime in established parliamentary democracies. Parliamentary regime is a good indicator of an overall democracy type. Traditionally, three main types of parliamentary regimes have been described: Westminster-style, cooperative, and chaotic. These regimes have been associated with different logic of operation, different degree of intra-institutional political conflict, and different models of responsiveness. Moreover, they have been seen as stable, fixed attributes. The paper argues that components of parliamentary regime[INS: , :INS] such as the degree of government dominance and the degree of intra-parliamentary cooperation[INS: , :INS] can vary significantly in time. In a wider context, they can contribute to our understanding of [INS: the :INS] long-term evolution of representative democracy. [INS: :INS] Inspired by recent debates on the types of democracy, the paper introduces a research framework based on a longitudinal analysis of two institutional features: (1) government dominance in key parliamentary committees[INS: ; and :INS] [DEL: , :DEL] (2) cohesion of government in parliamentary voting. In the preliminary analysis, the paper analyses data from five established parliamentary democracies (known for different typical coalition patterns) [DEL: from the period of :DEL] [INS: over the :INS] last twenty years. The results indicate that while the degree of dominance in parliamentary committees remains stable, the levels of voting cohesion keep changing significantly. Therefore, types of parliamentary regime and types of responsiveness in established countries are indeed evolving. (7) Martin Vrubel Barriers to school inclusion of students with visual impairment in high schools This article describes how high schools are prepared for the inclusion of students with visual impairments. It focuses on the importance of school management attitudes to [DEL: the :DEL] inclusive education. Furthermore, it discusses the impact of school management on inclusive education. This article also describes our research o[INS: n :INS] [DEL: f :DEL] the topic of inclusion, which was carried out in cooperation with 250 heads of high schools in the East Bohemian Region[JM9] . The paper summarizes the phase[JM10] of a larger research project, which deals with the issue of inclusion of students with visual impairments. This paper presents some of the partial results, such as the comparison between the significance of [DEL: headmaster :DEL] [INS: headteacher :INS] attitudes to the hard and soft barriers (external financial resources, teaching assistants, teacher training in special education, architectural barriers, teacher collaboration, attitudes of parents and children, and legislative support for inclusive education), and the importance of the same barriers to the particular institution. This text also presents [DEL: headmaster :DEL] [INS: headteacher :INS] attitudes to different types of visual impairment. (8) Durdica Marosevic In vivo spread of macrolides-lincosamides-streptogramin B (MLSB) resistance[INS: : :INS] [DEL: – :DEL] a model study INTRODUCTION: The extensive use of antimicrobial agents in animal husbandry poses a risk for the selection of resistant microorganisms. The gastrointestinal tract of animals [INS: h :INS] [DEL: w :DEL] as already[INS: been :INS] defined as rich in a variety of resistance genes and such animals can serve as reservoirs of resistant bacteria or genetic determinants of resistance that can be transmitted to humans. [INS: :INS] METHODS: Four week[DEL: s :DEL] old chickens colonized with Enterococcus faecalis carrying pAMβ1 (the challenge strain) were per orally exposed to three different antibiotics (tylosin, lincomycin and chlortetracycline) [DEL: during :DEL] [INS: for :INS] one week. Faecal samples and cloacal swabs were taken at four different time points and isolates of resistant E.faecalis and E.faecium were subjected to PFGE (pulsed-field gel electrophoresis) and plasmid isolation. The erm(B) gene was quantified in the DNA isolated from faeces by qPCR. The same DNA was subjected to 16S-rDNA pyrosequencing in order to g[INS: ain :INS] [DEL: et :DEL] better insights into the faecal microbiota composition and changes induced by the different antibiotics administered. [INS: :INS] RESULTS: A total of 77 enterococci and 22 streptococci were isolated. Two PFGE clones of each E.faecalis and E.faecium were observed, [INS: which were :INS] all different from the challenge strain. All isolates of enterococci and streptococci harboured a plasmid[DEL: of :DEL] the same size as that of pAMβ1. qPCR revealed an increase of erm(B) in all treated groups during the whole monitored period, whereas the challenge species E. faecalis rapidly declined over time. The impact of a non-specific antibiotic pressure represented by TET was similar to that of TYL and LIN[DEL: , :DEL] [INS: . F :INS] [DEL: f :DEL] urthermore, in two out of five chickens from the NCO group a significant increase of erm(B) was detected at the last sampling point. This is an [DEL: :DEL] interesting finding indicating that [INS: the :INS] spread of genetic determinants of MLSB resistance may be independent not only of the specificity of antibiotic pressure[INS: , :INS] but also of the antimicrobial pressure per se. A similar phenomenon was also observed under field conditions where MLSB resistant bacteria were isolated from piglets which had never before been treated with macrolides or lincosamides. The relative composition of faecal microflora was dependent on the antibiotic administ[INS: er :INS] [DEL: rat :DEL] ed and the sampling time point. [INS: T :INS] [DEL: Although t :DEL] h[INS: ese :INS] [DEL: is :DEL] results do not supply the exact information on the spread of MLSB resistance[INS: . They do :INS] [DEL: ; :DEL] however, as observed for enterococci, [DEL: the :DEL] [DEL: se :DEL] [DEL: :DEL] correlate[DEL: d :DEL] with the qPCR results. [INS: :INS] CONCLUSIONS: The erm(B) gene increased in all the treated groups and, therefore, it could be speculated that those bacterial taxons which prevailed under certain conditions might be considered as potential recipients. Thus it could be presumed that MLSB resistance can spread in different ways depending on the particular antibiotic pressure. (9) Tereza Ceskova [INS: The :INS] [DEL: A :DEL] Progress of Tools for [INS: the :INS] Investigation of [DEL: the :DEL] Learning Tasks in Czech Basic School Instruction [DEL: during :DEL] [INS: over :INS] [INS: the :INS] Last 25 Years Th[INS: is :INS] [DEL: e :DEL] poster presents [INS: the progress over a :INS] [DEL: a :DEL] 25[INS: :INS] [DEL: - :DEL] year[INS: period :INS] [DEL: -long progress :DEL] of tools for [INS: the :INS] [DEL: a :DEL] research of learning tasks in [DEL: the :DEL] basic school instruction in the Czech Republic. [INS: Learning tasks are :INS] [DEL: We :DEL] perceive[INS: d :INS] [DEL: a :DEL] [DEL: learning tasks :DEL] as [DEL: an :DEL] opportunit[INS: ies :INS] [DEL: y :DEL] to learn and [INS: it is :INS] [DEL: we :DEL] suppose[INS: d :INS] that one of their main function[INS: s :INS] is to activate pupils. [INS: A c :INS] [DEL: C :DEL] ontemporary conception of [DEL: the :DEL] Czech instruction[JM11] stresses not only [DEL: a :DEL] content knowledge but also [INS: the :INS] [DEL: a :DEL] developing of [DEL: the :DEL] key competencies. [INS: :INS] (10) Eva Lysonkova DEMOCRATICALLY-RUN BUSINESSES IN THEORY AND IN REALITY Due to the development of society, technology, work characteristics and the change of employees and employers requirements of work[INS: , :INS] new alternatives are emerging within the modern approach to human resource[DEL: s :DEL] management. The concept of democratically-run businesses [JM12] is one of these approaches. This article aims to introduce and define the concept of democratically-run [JM13] businesses. Firstly democratically-run businesses are characterized[DEL: , :DEL] [JM14] [INS: S :INS] [DEL: s :DEL] econdly they are defined from the perspective of theory and thirdly from the point of view of practitioners. Lastly, the article mentions the main reasons for the further investigation of democratically-run businesses[JM15] . (11) Saman Pushpakumara Edmund Husserl’s Transcendence of the Early Buddhist Theory of Consciousness [DEL: :DEL] Edmund Husserl[INS: (1859-1938) :INS] , the founding father of[DEL: the :DEL] western phenomenology, [DEL: had :DEL] formulated a theory of the consciousness[JM16] . The Gautama the Buddha, too as far back as the 6^th century B.C., [DEL: had :DEL] provided an analysis of the conscious phenomena. Many scholars who trace parallels between the Buddhist view of consciousness and Husserl’s phenomenology deal mostly with the similarities between the two philosophies. The present paper argues that Husserl's analysis of consciousness, despite its limitations, is more advanced than the Buddha's formulation. Husserl articulated his phenomenology of consciousness as a result of his encounter with Cartesian cogito on the one hand and the positivistic foundation of empirical sciences on the other. Husserl’s notion of phenomenological consciousness was situated within an industrially advanced capitalist society and nurtured by scientific epistemology. He was living in a different Time-Space [JM17] dimension, which has to be taken seriously [JM18] when assessing his phenomenology. The Buddha, in contrast, theorized his notion of consciousness within a backward, slow-moving, agricultural and feudal setting[INS: . He :INS] [DEL: , and :DEL] developed his notion of consciousness as a normative concept[DEL: , :DEL] as a basis for achieving the spiritual objective he envisaged. While acknowledging the fact that no other philosophy[DEL: , :DEL] [DEL: which :DEL] [INS: that :INS] existed during the Buddha’s time[DEL: , :DEL] [JM19] had articulated such a meticulous and in depth analysis of the phenomenology of consciousness, his analysis seems to be less advanced when assessed and compared with the twentieth century phenomenology of Husserl. [INS: The p :INS] [DEL: P :DEL] resent study will be carried out having used original and secondary sources of Husserlian and Buddhist phenomenologies. This research will [DEL: immensely :DEL] contribute [INS: significantly :INS] to [DEL: the :DEL] future research[DEL: es :DEL] on similar topic[INS: s :INS] . (12) Blanka Kubesova TP53 mutated clones in MPN patients Myeloproliferative neoplasms (MPN) are [INS: chronic :INS] diseases [DEL: with chronic course but :DEL] [INS: that carry :INS] [DEL: with :DEL] a risk of transformation into secondary acute myeloid leukemia. Several mutations and cytogenetic aberrations [DEL: were published to be :DEL] [INS: have been :INS] associated with [INS: the :INS] transformation[INS: , :INS] but its mechanism still remains unclear. Lesions in the pathway of the gene TP53 are considered to play an important role in the transformation. [DEL: Part of the :DEL] [INS: Some :INS] MPN patients [INS: are :INS] [DEL: is :DEL] treated long term with low doses of hydroxyurea as a cytoreductive agent. This drug was reported by several authors as mutagenic and leukemogenic and associated with [DEL: the :DEL] defects in the gene TP53 and transformation. [INS: :INS] [DEL: In our :DEL] [INS: This :INS] study [DEL: are :DEL] included patients in [INS: a :INS] chronic phase of the disease. The aim is[JM20] [DEL: :DEL] to identify, analyse and monitor patients with mutations in the gene TP53. Consequently the frequency of the occurrence of TP53 mutated clones, the relationship of their presence and the evolution of the disease and the type of treatment are evaluated. [INS: :INS] Leukocytes are obtained from patient blood samples. Subsequently the RNA is isolated from these samples and transcribed into cDNA. Then the samples are analysed by the method FASAY (functional analysis of separated alleles in yeast) and yeast colonies containing [INS: the :INS] mutated TP53 gene are sequenced by Sanger sequencing. [INS: :INS] Mutations in the gene TP53 were found in 3/18 patients treated [INS: with :INS] [DEL: by :DEL] hydroxyurea and in 1/22 patients treated [INS: with :INS] [DEL: by :DEL] [INS: a :INS] different cytoreductive drug. In one patient treated by hydroxyurea the mutated clone expanded by the time of monitoring and the second healthy allele was almost lost. Clinically the patient is stable. Current results suggest that [DEL: the :DEL] treatment with[DEL: :DEL] hydroxyurea could represent a selection pressure supporting the expansion of clones with [INS: the :INS] mutated gene TP53. ________________________________ [JM1]Is this a team study or individual. We speaks of team research and contemporary writing favours a noun phrase or passive construction to emphasise the importance of process over person [JM2]If this was a passive, it would have „were observed“ at the end of the sentence. The process is more important here [JM3]Noun compounds like the first two in this sentence usually only need the plural on the final noun [JM4]Would this be better as a passive? [JM5]Is this a single noun phrase or does it use parallel to describe a process or position? [JM6]What are you referring to here? [JM7]What is the process here and who are the people? Teachers, policy makers, parents? [JM8]How does this reflect or support the realization mentioned earlier? Add some info on the purpose of the articles [JM9]add dates to indicate length of study [JM10]what does this indicate, stage, time or both? [JM11]Instruction in the Czech Republic. Czech instruction sounds like language instruction [JM12]add a relative clause after this to briefly define the concept, e.g. „...businesses, which employees... is one of these approaches“ [JM13]Does this need to be hyphenated? As adverb-verb it would not usually have the hyphen, unless this is the way it is referred to in the field [JM14]Characterized as what? We usually add a examples with this expression. I’m suggesting a sentence break here as it will be a long sentence after inserting the example [JM15]You could also give examples of the main reasons if the word count allows. It would be a useful value statement to end on [JM16]Can you give the date he first published this? [JM17]does this need capital letters? [JM18]Can you give a brief comment on why at the end of the sentence? [JM19]This is not a relative clause so does not need the two commas. [JM20]Is this a current study or a completed study? Either make the previous sentence present or this one past, to match the time relationship grammatically. Look at this for the following sentences too which are present and sound like they may be completed. As a general methodological procedure relavant to any study we use the present, but for a specific study where results are known we use the past. The final paragraph confirms completion and need for the past