BIOCHEMISTRY II VSBC041c LABORATORY CLASSES 4^th Semester 2009 Spring 2009 Date Experiments 1 16 Feb. 20 Feb. Directions for laboratory work. Handling biological material. 1.1 Blood collection. 1.2 Blood processing. 1.3 Manual methods, pipetting. 1.3 Biochemical analyzers. 1.4 Interpretation of test results. 1.5 Test requisition forms 2 23 Feb. 27 Feb. Investigation of lipid and cholesterol metabolism 2.1 Determination of total cholesterol in serum and blood. 2.2 Determination of blood triacylglycerols usány Reflotron . 2.3 Determination of HDL-cholesterol and LDL-cholesterol. 2.4 Calculation of LDL-cholesterol 2.5 Electrophoresis of serum lipoproteins. 3 2 Mar. 6 Mar. Investigations of glucose metabolism. Diabetes mellitus 3.1 Enzymatic determination of serum glucose. 3.2 Determination of glycaemia by personál glucometer. 3.3 Oral glucose tolerance test (oGTT). 3.4 Detection and determination of glucose in urine. 3.5 Detection of ketone bodies in urine. 3.6 Determination of glycated haemoglobin (HbA1). 3.7 Late complications of diabetes – microalbuminuria. 4 9 Mar. 13 Mar. Investigations in liver disease I– Enzymes 4.1 Determination of ALT catalytic conncentration in serum. 4.2 Determination of AST catalytic conncentration in serum. 4.3 Determination of ALP catalytic conncentration in serum. 5 16 Mar. 20 Mar. Selected investigations in liver disease II – bile pigments and albumin 5.1 Determination of total bilirubin in serum. 5.2 Detection of bilirubin in urine. 5.3 Detection of urobilinogens in urine. 5.4 Determination of serum albumin. 6 23 Mar. 27 Mar. Laboratory diagnostics of myocardial infarction 6.1 Determination of creatine kinase ctalytic concentration. 6.2 Determination of serum CK-MB catalytic concentration. 6.3 Determination of troponin T in blood. 6.4 Determination of blood myoglobin. 7 30 Mar. 3 Apr. Investigation of plasma proteins 7.1 Determination of total protein by the biuret test. 7.2 Determination of blood albumin in serum. 7.3 Electrophoresis of serum proteins. 7.4 Assay for increased level of C-reactive protein. 7.5 Determination of serum IgE by ELISA method. 8 6 Apr. 10 Apr. Biochemical tests of renal function – I 8.1 Investigation of urine by inspection and physical methods. 8.2 Determination of creatinine in serum and urine. 8.3 Measurement of glomerular filtration rate with creatinine. 8.4 Fractional reabsorption and excretion of water. 9 13 Apr. 17 Apr. Biochemical tests of renal function – II 9.1 Detection of proteinuria. 9.2 Urine test for haemoglobin (haemoglobinuria and haematuria). 9.3 Determination of protein concentration in urine. 9.4 Detection of microalbuminuria. 9.5 Determination of urea in serum and urine. 9.6 Approximate assessment of the nitrogen balance. 10 20 Apr. 24 Apr. Routine urinalysis. Test strips 10.1 Multipurpose diagnostic strips in urinalysis. 10.2 Detection of addictive drugs in urine. 10.3 Detection of barbiturates in urine. 10.4 Detection of luteinizing hormone in urine 11 27 Apr. 1 May Examinations in in urolithiasis 11.1 Solubility of uric acid. 11.2 Detection of uric acid by murexide reaction. 11.3 Simplified chemical analysis of renal calculi. 11.4 Determination of uric acid in serum and urine. 12 4 May 8 May Some investigations of the gastrointestinal tract 12.1 Selected examinations of stomach functions. 12.2 Laboratory tests in pancreas diseases. 12.3 Breath tests in gastroenterology. 12.4 Test of occult bleeding in gastrointestinal test. 13 11 May 15 May Compensatory lesons 14 18 May 22 May Compensatory lessons. Selected topics. 15 25 May 29 May Credits. Neglected lessons have to be made up by the 19^th of June 2009. Students are expected to come to the seminary room at least 5 minutes before the start of the lesson. Attendance in lessons is obligatory. All absences must be justified through the Department of study affairs up to 5 days! Illness is usually the only acceptable excuse for absence from class and must be officially confirmed. After being absent the student must make up the given topics according to the teachers instructions. Conditions for giving the course-unit credit · full attendance in the all lessons · presentation of all lab reports to the teacher Obtaining of course-unit credits of practices is the pre-requisite for registration to the examination of Biochemistry II.