BIOCHEMISTRY II LABORATORY CLASSES SPRING 2015 Dentistry ZLBC041c Week Date Experiments 3 4. 3. Directions for laboratory work. Investigation of glucose metabolism 1.1 Investigation of glucose metabolism 1.2 Enzymatic determination of plasma glucose 1.3 Determination of glycaemia by personal glucometer 1.4 Test for glucose in urine 1.5 Test for ketone bodies in urine 6 25. 3. Investigation of hard tissues 2.1 Determination of calcium in serum 2.2 Determination of total ALP catalytic concentration and bone ALP isoform catalytic concentration 9 15. 4. Investigation of saliva 3.1 Investigation of salivary secretion rate 3.2 Estimation of salivary buffering capacity 3.3. Detection of selected organic compounds in saliva 3.4. Detection of a-amylase in saliva and its substrate specifity 13 -14 11. 5. – 22. 5. Compensatory lessons. Credits. Recommended literature: Biochemistry-Practicals: Information system of MU (is.muni.cz) - Study materials Conditions for giving the course-unit credit * Full (100%) attendance in labs is the principal condition. If any absence, it must be apologized through Department of Study Affairs up to five days. If apology is recorded in Information System (IS), then student is allowed to make up the absence according to teacher's instructions. * Presentation of all lab reports to the teacher Deadline for any issue (making up all missing and justified lessons, completion of all lab reports and handing them to the teacher) is 11. 6. 2015. Students that will not meet this requirement will not be given the course-unit credit. Obtaining of course-unit credits of practices is the pre-requisite for registration to the examination of Biochemistry II. [DEL: :DEL]