MEZINÁRODNÍ CENTRUM KLINICKÉHO VÝZKUMU „TVOŘÍME BUDOUCNOST MEDICÍNY“ Transformation of chronic atopic dermatitis to a cutaneous T- cell lymphoma: a case report M. Nečas, V. Vašků 1st Dept. of Dermatovenereology, Masaryk University Faculty of Medicine, and St. Anne’s Faculty Hospital in Brno Patient T.B., born in 1983 Family history: father hay fever , cardiac arrhythmia mother: healthy siblings: brother - asthma, atopic dermatitis, allergy to pollen children: 0 Personal history: common childhood diseases asthma until 10 years FA: Symbicort 200 inh. Pulv., Xyzal 1-0-0, 0-0-1 Dithiaden AA: pollen, animal dander, dust mites Abuse: smoker, little alcohol, coffee once daily PP: height 181 cm, weight 58 kg, PSA: Logistics at Pelikan Hard Copy comp., Kyjov, Lives with his wife in an apartment house. Animals: water turtle Course of the disease Atopic dermatitis since early childhood / since 4 years of age/ Since 2009 erythroderma IgE 2300 IU/ml (2010) since the end of 2010 reccurent herpetication of AD, thererefore topical KS discontinued. Treaterd repeatedly with acyclovir Severe itching - antihistamines, sedatives, with minimal effect 8/2010 LN biopsy /left axilla/ - benign lymphadenopathy within AD Immunophenotyping of periferal blood T cells (flow cytometry) - normal Course of the disease Treated with phototherapy: UVB 311 in spring 2010 for two months and in spring 2011 Inguinal lymphadenopathy appeared in Aug 2011 Histology of the skin in the right groin : epidermotropic peripheral T- cell lymphoma - mycosis fungoides, patch stage Lymh node biopsy /right groin/ histological picture compatible with dermatopatic lymphadenopathy within MF / SS, LN involvement is proportional to category I. Aug 2011 Aug 2011 Aug 2011 Aug 2011 Sept-Oct /2011 Total IGE 11 942 IU/ml Immunophenotyping of periferal blood T cells /Flowcytometry/: CD 3-+4+7 (MF) cells make 7,82 % of lymphocytes Index CD4/CD8 2,157 Biopsy from right thigh: chronic dermatitis of AD type. Signs suggestive of mycosis fungoides are insufficient in this preparation. Material submitted for a second reading and examination of clonality Clonality test result was unassessable. 0ct-Dec/2011 PUVA : end of 10/2011 to half 12/2011 - terminated because of irritation 5/2012 erysipelas of the right thigh, treated with procaine penicillin G for 10 days, at the end benzathin PNC 1,5 MIU applicated. Later 5 or 6 more erysipelas at the same site happened, probably because of removed LN in the right groin, treatment with benzathin PNC was introduced - pendeponization Total IgE 8 030 IU/ml flowcytometry - CD3 + 4 + 7 – MF cells make 6.35 % of lymphocytes index CD4 / CD8 2,612. July-Sept/2012 July/2012 PET/CT: slightly increased metabolism in both axillary and inguinal lymphnodes compatible with suspected low grade lymphoma Total IgE 5 837 IU/ ml 9/2012 biopsy from the left trunk : The finding corresponds with subacute atopic eczema. For the diagnosis of mycosis fungoides there are not enough signs. Yet sorting of the cells near basement membrane may preceed the development of lymphoma. Clonality assessment not possible Sept/2012 biopsy from left axillary LN : negative for MF Jan-May/2013 Phototherapy UVA- 1 from Jan 2013 to end of May 2013 Total IgE 6 564 IU/ ml Immunophenotyping of periferal blood T cells /flowcytometry/: CD3+4+7- cells make 7,67% of lymphocytes Index CD4/CD8 3,324 PET / CT shows stationary finding of a higher FDG activity in LN of both axillary and groin LN, it might be an activity accompanying a low grade lymphoma Sept/2013 Biopsy from the skin lesions in the right forearm Skin excision with psoriasiform hyperplasia of the epidermis covered by slightly extended hyperkeratotic slats with small areas of parakeratosis. Papillary dermis slightly expanded, with a vaguely nodular molded medium-dense infiltrates of small and medium-sized lymphoid cells somewhere with irregular contours of nuclei, inconspicuous focal pigment incontinence. Sporadically, the admixture of eosinophilic granulocytes. In the epidermis rare isolated cells of lymphoid appearance with cerebriform appearance of nuclei. There is no sorting of lymphoid cells on the dermo-epidermal junction, no intraepidermal aggregates of lymphoid cells. Reticular dermis without alteration. Conclusion: rather chronic eczema/dermatitis. Not all features which would allow an unambiguous diagnosis fulfilled. Progression to MF should be monitored. Histology Bohm 147 11.jpg Photo: ass. prof. Jedličková, Ph.D. Nov/2013-Jun/2014 UVA -1 from Nov/2013 to June/2014 IgE 9 164,0 kU/l 9/2014 Flow cytometry CD4+8+7- cells 8% index. 2,594 9/2014 biopsy from the forehead skin Epidermis with reduced straum corneum, distinct acanthosic pins. Beneath the epidermis there are dense spherical lymphohistiocytic infiltrates with deposits of pigment. Lymphocytes focally enter epidermis RES: Density of the infiltrates and the character of the invasion of lymphocytes into the epidermis is very suggestive of mycosis fungoides, material sent for immunostaining. Clonality test: Isolated DNA of poor quality. Clonality can not be assessed. Consider retesting for clonality and consultation in another laboratory. Histology bohm 108 14 a.jpg Photo: ass. prof. Jedličková, Ph.D. Sep/2014 Sep/2014 Sep/2014 Sep/2014 Nov/2014 Nov/2014 Treatment with acitretin 40 mg daily + interferon a gradually increased to 6 MIU 3 times a week s.c. Jan/2015 skin finding except the face satisfactory, significant reduction of lymphatic nodes Tolerance of the treatment - very good Laboratory findings satisfactory - slight elevation of LT and lipids acitretin reduced to 30 mg daily, interferon a to 4.5 MIU 3 times a week 1/2015 Jan/2015 PET CT: significant metabolic and size regression of axillary and inguinal LN higher metabolism of bone marrow especially in the pelvic bone and of the spine of unclear etiology. Several minor active areas in the S8 / 9 segment of the lungs –probably a postinflammatory change light progression of the spleen size PET CT Bohm 1 2015.jpg Feb/2015-May/2015 2/2015: bone marrow aspiration was performed from the left hip result: histologically in the bone marrow without an evidence of the infiltration of the bone marrow with a lymphoma flowcytometry - normal in myelogram rare atypical lymphocytes res: no ivolvement of bone marrow by a lymphoma 5/2015 acitretin reduced to 20 mg daily, interferon a left on dose of 4.5 MIU 3 times a week Laboratory: LT and cholesterol normalized Jun/2015-Sept/2016 6/2015 flowcytometry : CD4+8+7- 13% of lymphpocytes index CD4/CD8 3,538 10/2015 total IgE 5 770 IU/ml 5/2016 total IgE 6 305 IU/ml Lab: slight leucopenia (minim. 3,4 109/l) between 9/2015 and 11/2015 Interferon a reduced to 3 MIU and then to 1,5 MIU 3 times a week 7/2016 flowcytometry : CD4+8+7- 7,4 % of lymphocytes index CD4/CD8 2,647 Sept/2016 DSC_5843.JPG Sept/2016 DSC_5839.JPG Sept/2016 DSC_5838.JPG Sept/2016 DSC_5837.JPG Classification of CTCL Primary cutaneous T-cell and NK-cell lymphomas Mycosis fungoides Variants of mycosis fungoides: Folliculotropic mycosis fungoides Pagetoid reticulosis Granulomatous slack skin Sezary syndrome Leukemia/adult T-cell lymphoma CD30+ T-cell lymphoproliferative hyperplasias: CD30+ anaplastic large T-cell lymphoma Lymphomatoid papulosis Subcutaneous panniculitis-like T-cell lymphoma Extranodal NK/T-cell lymphoma, nasal type Primary cutaneous peripheral T-cell lymphomas, unclassified Primary cutaneous aggressive epidermotropic CD8+ T-cell lymphoma Cutaneous γ/δ T-cell lymphoma Primary cutaneous small/medium CD4+ T-cell lymphoma Discussion AD associated CTCL – quite rare: CD30+ lymphomas ( 8 cases) Sezary syndrome ( 2 cases) Mycosis fungoides ( 3 cases) Probably underdiagnosed (or unpublished) Discussion •Mechanisms leading to the development of malignant T cell population: • •Chronic antigenic stimulation (of cutaneous •lymphocytes) or chronic inflammation itself •Immunosupressive nature of the disease, •Treatment (cyclosporine, TIMs) •Phototherapy?? • Conclusion In a long lasting severe AD dermatologist must be aware of posssible transformation of the condition to a CTCL especially if the diseases does not react properly to usual treatment of AD Repeated histology, immunophenotyping, flow cytometry, TCR gene rearrangement (clonality) Staging (X-ray, CT, PET/CT, US of LN or biopsy, bone marrow biopsy or trepanobiopsy) Markers to differentiate AD/CTCL sIL-2R, serum LDH, IgE - elevated in both Low specific IgE, high CD4/8 ratio - usualy in CTCL CCL 17 (TARC) – CCL 8 or CCR 4 elevated in both CCR 11 and CCR 26 – CCR 3 elevated in both CCL 27 (CTAC) – CCR 10 elevated in CTCL FoxP3+ regulatory T cells (T regs) elevated in AD not CTCL Hanafusa T, Matsui S, Murota H, Tani M, Igawa K, and Katayama I. Increased frequency of skin-infiltrating FOXP3+ regulatory T cells as a diagnostic indicator of severe atopic dermatitis from cutaneous T cell lymphoma. Clin Exp Immunol. 2013 Jun, 172(3): 507-512 MEZINÁRODNÍ CENTRUM KLINICKÉHO VÝZKUMU „TVOŘÍME BUDOUCNOST MEDICÍNY“ Thank you for your attention contact: miroslav.necas@fnusa.cz St. Ann´s Faculty Hospital Brno Pekařská 53, Brno 656 91 Czech Republic Phone: + 420 543 181 111 www.fnusa.cz CZ1411270226/11/2014