Syllabus – Practical Exercises of Biophysics 2021


                                           Laboratory 1

(students work in smaller groups according to the schedule, detailed instructions for individual
tasks are in the IS)


Task I. Basics optical laboratory methods

Spectrophotometry – Absorption curve and determination of concentration of eosin

Refractometry – Determination of NaCl concentration

Optotypes – visual acuity


Task II. Properties of liquids

The surface tension of liquids.

Viscosity measurement.


Task III. Ultrasound and ionising radiation

Hemolysis of erythrocytes by ultrasound

Measuring ionising radiation absorption


                                           Laboratory 2

 (students work in smaller groups according to the schedule, detailed instructions for individual
                                       tasks are in the IS)


Task IV. Electrical parameters

Measuring the voltage and frequency of electric signals with the oscilloscope

Frequency dependence of tissue and tissue model impedance.

Skin resistance measurement


Task V. Monitoring 1

Measurement of skin temperature – Thermocouple,

Blood pressure measurement by the auscultation method


Task VI. Monitoring 2

Conductometry – electrical parameters of membranes

Audiometry – hearing, sound intensity and loudness

Blood flow measurement by the Doppler ultrasonic flowmeter


                                       Collective Exercises

This tasks we will do together, so it is very important to prepare theoretically and use for
preparation all accessible sources. Main and most important source for you is the Fundamentals of
Biophysics and Medical Technology (Hrazdira et al, 2012), but many information you can find at the
other places (libraries, internet…).


Task I. Electromagnetic radiation and its perception.

Required knowledge:

Properties of electromagnetic radiation including basic formulas. Spectrum of the EMR.

Measurement of the illuminance.

Thermoregulation of a human body.

Contact and contactless thermography. Thermocamera and thermovision.


Task II. Electric currents. ECG measurement. Physiotherapy.

Required knowledge:

Properties of electric currents. Effect of the direct and alternating currents to the biological
objects. Electrotherapeutic methods. Electrical excitability. Electrodiagnostic methods (active,
pasive).

Physics in physiotherapy (magnetic field, laser and others).


Task III. Imaging methods. Ultrasound imaging of the gallbladder. X-ray images.

Required knowledge:

Properties and production of ultrasound. Medical applications of ultrasound - diagnostic and
therapeutic usage. Doppler effect and medical applications - velocity of the blood flow.

Properties of X-rays and medical applications including CT.

Principle of NMR and MRI.

Types of the endoscopes