Influence of light on compounds content in Tradescantia pallida

Anthocyanines – detector 535nm


Chlorophyll – detector 450 nm

cultivation

                                                                                Anthocyanine - peak areas – fresh material

                                               light

                                                                                                   44.7

                                               dark

                                                                                                    9.9


cultivation

                                                                                 Chlorophyll - peak areas – fresh material

                                               light

                                                                                                   92.6

                                               dark

                                                                                                   102.5



Task:

1)      Calculate the relative concentration of anthocyanins and chlorophyll in dry mass and
expressed it graphically.

2)      Calculate the % of water content

Water content is the basic analytical value of the composition of plant tissues. It is carried out
by drying the plant or its parts at a temperature of 105 ° C to constant weight. The basis is to
determine the weight loss that corresponds to the water content. The rest is dry matter composed of
organic and inorganic substances.


% of water content = (1-        weight of dry material ) x 100

                                               weight of fresh material




plant

cultivation

weight of fresh material

weight of dry material

% of Water content

anthocyanin


anthocyanin

(0.1 g)

chlorophyll

chlorophyll

(0.1 g)

Tradescantia pallida


Light

5,857


0,274

95,3

44,7

16,31

92,60

33,80

Tradescantia pallida

Dark

3,729

0,484

87

9,9

2,05

102,5

21,18