Innovation Lectures (INNOLEC)


                                              IC041:
  Light Emitting Diodes in Chemical Analysis, Detection, Fluorescence Microscopy, Visualization,
                                    Photochemistry and Teaching


                                         Doc. Mirek Macka

                 School of Chemical Sciences, Irish Separation Science Cluster and
            National Center of Sensor Research, Dublin City University, Dublin, Ireland

                                   e-mail: mirek.macka@gmail.com




                                   1. Tue 30.3. 2009 9:00-12:00

                                   2. Tue 30.3. 2009 13:00-17:30

                                   3. Wed 31.3. 2009 9:00-12:00


                             Lecture hall, Dean’s office, Kotlářská 2



This interdisciplinary short course is an intensive introduction to the properties and usage of
light emitting diodes (LEDs) in chemical analysis and chemistry in general.  The course will cover
the background and theory and then will focus on practical issues and the applications of LEDs
particularly for analytical optical methods (photometry, fluorimetry), optical detection in
analytical flow-through methods (FIA, HPLC, CE), optical sensors, microfluidic chips, fluorescence
microscopy and visualization in photochemistry and teaching.  Examples of some elementary
techniques used to characterise LEDs including measurement of emission spectra will be conducted as
hands-on experiments.

This is a both beginners and intermediate level course with respect to LEDs.  The course is
designed in such a way that a wide range of participants can benefit: those curious about the use
of LEDs in chemistry and science, those starting to use LEDs, having some experience, and students,
scientists, engineers or lecturers considering whether LEDs could be used in their specific work.
The course is also suitable for persons involved in teaching in the areas of instrumental
analytical chemistry, physical chemistry and photochemistry.  The participants will benefit from
some background in chemistry and analytical chemistry in particular, but this interdisciplinary
course is designed in such a way that students or professionals with other background can
participate and benefit as well.  Prior exposure to and use of other LEDs is useful but not
expected or necessary.


Course text: Class notes; readings to be assigned.

Reference texts and websites:

Gilbert Held, Introduction to Light Emitting Diode Technology and Applications, CRC Press, Boca
Raton, 2009.

http://en.wikipedia.org/wiki/Light-emitting_diode

http://www.ledsmagazine.com/

http://ca.pittcon.org/Technical+Program/tpabstra10.nsf/SCoursesByCat/CB1DD8E4B5476AD7852575E700803F
47?opendocument http://electronics.howstuffworks.com/led.htm
http://www.viewsfromscience.com/documents/webpages/led_fluorescence.html
http://www.microscopy-analysis.com/features/led-light-sources-major-advance-fluorescence-microscopy


Kontaktní osoba: doc. Jan Preisler, Ústav chemie, kl. 6629; e-mail: preisler@chemi.muni.cz

                                             Syllabus


Lectures 1, 2 and 3


  * Why use LEDs in chemistry and science?
  * History
  * Fundamentals (physical principles, construction)
  * Basics of LEDs in respect to their applications in analysis and chemistry
  * Application areas
  * Considerations in choosing a LED
  * Practical issues when using LEDs
  * Coupling of LEDs with optical fibers
  * Pulsed techniques: use of lock-in amplifiers and LEDs in TRF (time resolved fluorescence)
Optical methods in chemical analysis with an emphasis on photometry and photometric detection,
fluorimetry and optical detection methods in microfluidic chips
  * Basics of construction of simple LED-based photometers and fluorimeters
  * Fluorescence microscopy and visualization using a set-up for <@250
  * Diode lasers for compact inexpensive LIF (Laser Induced Fluorescence) detection in on capillary
and microfluidic chip formats
  * LEDs as light sources for photochemistry
  * Other advanced and specialized methods
  * Hands-on experiments: measuring emission spectra, optical power, intensity, transmittance,
absorbance, pulsing LEDs and use of lock-in amplifier techniques
  * Workshop on examples from participants’ own specific usage, questions, discussion

  * Workshop using examples incl. particular applications of course participants
  * Consultations